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ENGINEERING

No one can make great progress without advice and golden ideas from their excellent partners.

OVERVIEW

This year XMU-China have tackled and solved one or more of our project's problems and use synthetic biology tools to generate expected results. Our project contains three systems: detection system, degradation system and kill switch systems.

After brainstorming and design improving through literature analysis and Human Practices(See the Human Practices details in Human Practices), we found several enzyme candidates. Totally, 101 new BioBricks were designed for our project (See the Parts and Design details in Parts and Design ).

We have done a lot of amazing work to make sure that they can realize the goals we set. Moreover, it is worth mentioning that BBa_K3332006, BBa_K3332011 and BBa_K3332028 are typical among them. These three BioBricks works well to perform their functions and works as expected. All data related are recorded here. We hope they will make some contribution to the iGEM community.

ENGINEERING SUCCESS

A. Detection System

1. GRHPR-his-tag (BBa_K3332006)

There is more than one enzyme with hydroxypyruvate reductase activity in humans. We choose one of these proteins which is found from human liver called GRHPR reduce glyoxylate when NADPH is used as cofactor. J23100-B0034 is used to construct the express circuit, and the GRHPR protein is purified and used to test the enzyme activity.

1-1. Agarose Gel Electrophoresis

Result: This target gene, GRHPR is designed successfully as the gragh shown below.