Team:NPU-CHINA/human practice.html

project

OVERVIEW

This year, our program focuses on the safety in food production. Considering the local situation and brainstorming in the team, we decided to solve the problem of herbicide residues in tea production. First of all, we interviewed the practitioners in different production links in the tea industry, and they provided valuable opinions for the design and implementation of our project. In the process of project design, we exchanged views with professionals from universities and government departments, covering different fields such as biology, environment and ecology, and mathematics. At the same time, we also kept close contact with other universities and shared a lot of valuable information. In order to integrate our project into the society and promote the ideas and methods of synthetic biology, we conducted public education on synthetic biology through offline activities and distributed questionnaires related to the subject through online channels. In the process of all the above activities, we have ensured the information security of the participants according to the guidance of professionals.

In the whole HP activity, every step complements each other and forms an organic whole.

HUMAN PRACTICE

Background

Introduction

Simply state that there is an aflatoxin problemAnd selenium
Social research

Aflatoxin understanding
Rancher understands the situation

Telephone interview with Mr. Gan

Telephone interview with Longjia Animal Husbandry
Feed mill

Field interview with Yangling Tiandi Animal Feed Factory

The problem of adding inorganic selenium to feed
Summarize

Need to degrade aflatoxin

Project Design

Teacher Weining Niu

Degradation may be achieved through genetic engineering of strains. Consider using engineered bacteria to solve the problem
Teacher Junling Shi

The problem of strain selection believes that Bacillus subtilis is safe and has an engineering basis
Teacher Xiaoya Shang

Introduce that the intestinal environment can become a reaction site for engineered bacteria
Teacher Yanpeng Li

There are problems with the release of degrading enzymes and growth control problems. It is recommended to add a self-lysis system
Teacher Qingsheng Huang

Safety issues, there are very mature means of detecting aflatoxin

Exchange Discussion

Northwest Region Exchange

I met many teams, got to know each other's work, made suggestions, and established cooperative relations
CCiC

CCiC is the abbreviation of Conference of China iGEM Community. This year's seventh CCiC could not be held offline due to the epidemic situation. Sixty teams attended the conference on August 28-29 through online conference and video display, and shared their respective topics. During the meeting, we actively maintained communication with other teams, and gained many valuable suggestions while making suggestions.

The team members are participating in the discussion of the online meeting

After the project exhibition, the judges put forward helpful suggestions on our presentation method, and affirmed our report effect.

Participating in CCiC and getting the best poster award

INTERGRATED HUMAN PRACTICE

In-depth analysis of the questionnaire

Provide solutions through performance analysis

Project construction

Choose to use prokaryotic organisms (subtilis) to express degrading enzymes (Teacher Niu, Yang Ling thinks it can be resolved after communicating with Tiandi)

Some microorganisms

are rich in selenium
Ability consultation teacher

Xu Chunlan withered grass bud
Some microorganisms

are rich in selenium

Construction system consulting Dr. Xu Ming feasibility

Value orientation of the project

In the process of project promotion and improvement, we always put safety in the first place. The vision of our project is to promote the use of synthetic biological detection and degradation of glyphosate on a large scale in the society. Once these new methods are used on a large scale, any slight potential safety hazard may turn into a problem that seriously endangers public safety. Therefore, the demand for safety is at the highest position in our project. This is reflected in our HP and modeling work in kill switch, and this problem is clearly explained in the questionnaire distributed to the public.

When the project is implemented, we hope to protect the benefits of users (tea farmers, tea merchants, etc.) to the greatest extent. This point can be fully reflected by our HP design: we have adopted many suggestions from tea industry practitioners. And in the process of HP, we were also moved by the industrious and honest quality of these workers, thus strengthening our previous ideas.

Because our project uses mature technology and does not directly edit the genes of animals and plants, it will not introduce ethical issues.

Close the Loop

When carrying out HP activities, we pay great attention to translating other people's suggestions into the design in our subject, thus forming a closed loop between other people's expectations and our design. These positive feedbacks are shown in the following figure.

CONTACT NPU CHINA

NPU-CHINA is the iGEM team of Northwestern Polytechnical University

Address: 127 West Youyi Road Beilin District, Xi'an,

Shaanxi 710072, China

Contact: npuigem@outlook.com