Americans face food insecurity or have limited access to affordable and fresh produce. These people are limited to either which have been linked to chronic illnesses such as obesity, diabetes, and heart attacks. Food insecurity is highly prevalent in our hometown of Atlanta, with one in every four Atlantans living in neighborhoods classified as food deserts. , but maintenance and costs are barriers to implementation.
THE FUTURE OF FARMING
Building upon Lambert’s 2020 Nar part, we created a cell-free system to ensure that our sensors are safe to distribute in the agricultural market.
We characterized our 2020 Pho biosensor for concentrations common to hydroponic systems in order to improve sensitivity and provide a cost-effective method of phosphate monitoring.
PLANT PATHOGEN BIOSENSORS
In order to address other pervasive issues found in hydroponics, we created two toehold biosensors to detect Fusarium oxysporum and Phytophthora cryptogea, two common plant pathogens in both hydroponics and soil-based farming.
We created an Ordinary Differential Equation nitrate model that simulates GFP expression in response to various concentrations of nitrate. We also created an Ordinary Differential Equation plant pathogen biosensor model to predict the concentration of GFP produced in response to pathogenic presence.
We developed a cost-effective lyophilizer to freeze-dry our nitrate and phosphate biosensors to significantly lower biohazard concerns surrounding bioengineered products and increase their shelf-life.
To complement our biosensors, we developed a cost-effective plate reader to quantify fluorescence concentrations expressed from our biosensors to precisely monitor nutrient and pathogen levels.
Agriculture is essential to the preservation of life and is vital that modern farming techniques are able to accommodate growing populations while maintaining both the economy and the environment. AgroSENSE centers around the pursuit of sustainability through all aspects of our project.