With advances in synthetic biology and gut microbiology, many scientists plan to engineer strains to function in the gut. However, the genetic background of common probiotics is unclear and difficult to engineer. The common e. coli has internal toxicity and is not suitable for human or animal intestinal function. So we used 1917, which had the advantage of being engineered without endotoxicity. We tried 1917 and it worked, which gave other teams the insurance, confidence and experience to use 1917. In our project, we successfully implement INP-N(ice-nucleation protein) in the Microbial Cell Surface Display System to create a whole-cell bioeatalyst and prove the higher efficiency the recombined part expressed. INP is a widely used functional protein, having stably expressing ability of large weight exogenous proteins. Thus we applied it to our expression system which can be further employed in animals’ body system. There are many enzyme that are more appropriate to be expressed in the periplasmic space, and the success of our combination offer a model for further teams to use. Thus, our experimental outcome is very promising, which can be applied in many further iGEM projects to enhance the enzyme activity and promote experiment efficacy in both testing, examining and proving process.
P1,2 Nissle 1917.
p3,4 The figure of INP and its carrier protein