Team:HK SSC/Parts
Part Table
The below parts are used in our project. These parts include coding sequences codon optimized for S.elongatus UTEX 2973, the INTEGRATE system designed by Vo et al., and a shuttle vector. This year, our team was not able to characterize many of their functions due to restrictions in the laboratory so we will characterize them in the second phase of the project.
BBa_K3776006 enables 5-AVA synthesis in S.elongatus UTEX 2973. The feedback insensitive aspartate kinase allows lysine overproduction for synthesis of 5-aminovalerate by DavB and DavA.
BBa_K3776009 is a shuttle vector that harbors ColE1 and panS replication elements for E.coli and S.elongatus UTEX 2973. The MCS allows flexibility for cloning and the ColE1 flanked by loxP sequences can be removed by Cre recombinase.
BBa_K3776019 is the INTEGRATE system by Vo et al..It is a CRISPR-transposon system for genome editing.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K3776001 | Coding | DavB | Helene Heling Huang | 1683 |
| BBa_K3776002 | Coding | DavA | Helene Heling Huang | 795 |
| BBa_K3776003 | Terminator | TrbcL | Helene Heling Huang | 179 |
| BBa_K3776004 | Regulatory | 6803psbA2 | Helene Heling Huang | 415 |
| BBa_K3776005 | Coding | AK T369I | Helene Heling Huang | 1371 |
| BBa_K3776006 | Composite | davB-davA-AK | Helene Heling Huang | 5221 |
| BBa_K3776007 | silver | panS | Helene Heling Huang | 3059 |
| BBa_K3776008 | Coding | KanR | Helene Heling Huang | 816 |
| BBa_K3776009 | Plasmid | ppanS-loxP-MCS | Helene Heling Huang | 5687 |
| BBa_K3776010 | RNA | NSI | Helene Heling Huang | 894 |
| BBa_K3776011 | RNA | NSII | Helene Heling Huang | 1484 |
| BBa_K3776012 | Coding | CF3BD | Helene Heling Huang | 771 |
| BBa_K3776013 | Coding | cas8 | Helene Heling Huang | 1923 |
| BBa_K3776014 | Coding | cas7 | Helene Heling Huang | 1059 |
| BBa_K3776015 | Coding | cas6 | Helene Heling Huang | 600 |
| BBa_K3776016 | Coding | TnsA | Helene Heling Huang | 693 |
| BBa_K3776017 | Coding | TnsB | Helene Heling Huang | 1812 |
| BBa_K3776018 | Coding | TnsC | Helene Heling Huang | 993 |
| BBa_K3776019 | Composite | INTEGRATE system | Helene Heling Huang | 8883 |
| BBa_K3776020 | Plasmid | panS-SPIN-davBA-AK | Helene Heling Huang | 19837 |
| BBa_K3776021 | Plasmid | panS-SPIN-CF3BD | Helene Heling Huang | 16126 |
| BBa_K3776022 | Other | Vrepeat | Helene Heling Huang | 28 |
| BBa_K3776023 | Other | Right flank | Helene Heling Huang | 111 |
| BBa_K3776024 | Other | Left flank | Helene Heling Huang | 145 |
| BBa_K3776025 | Composite | TniQ | Helene Heling Huang | 1185 |