Group: Cornell iGEM 2021
Author: Abraham Sinfort, Jacob Novozhenets
Summary: This improvement is to Part:BBa_K2387032, which detects activation of the Cpx pathway of E. coli. Our modification adds a methyltransferase gene to the plasmid backbone. Since this plasmid is typically native to E. coli, transforming Bacillus subtilis — a common alternative chassis — with this plasmid would subject it to the host’s Restriction-Modification system. Since Bacillus subtilis’ R-M system targets foreign unmethylated DNA, we can prevent the degradation of this plasmid by using E coli. as an intermediary to facilitate methylation. While E coli already possesses basal methyl transferase activity, introducing this gene will dramatically increase the efficiency of this process; and in doing so, increase the efficiency of transforming vectors into alternate bacteria, such as Bacillus subtilis.
Resource: Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria
Modified Part
