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            <span dir="auto">Team:HUST-China/Human Practices 01</span>
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     <title>Education | iGEM HUST-China</title>
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     <title>Human Practices | iGEM HUST-China</title>
 
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             <h1 id="Top">Human Practice: Education and Communication</h1>
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             <h1 id="Top">Human Practices</h1>
             <p class="lead pl-1">Part 04  Broaden the impact: Community and Communication</p>
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             <p class="lead pl-1">Part 01 Discover and analyze the problems</p>
 
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                     <article>
 
                     <article>
                         <p>At the beginning, HUST-China set up Mr. Tony project with the exact goal of truly make
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                         <p>At present, perming and dyeing hair has become a trend and a new trend of beauty. However, in
                             contributions beneficial to the world, and strive to be responsible for people around the
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                             China, the popularity of perming and dyeing is not as expected. Many people have the desire
                             world. It means that at the end of completion of the project, it is important to broaden the
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                             to improve their image by perming and dyeing, but it has not been realized in the end. After
                             impact of the project on society and humanity. This is not only instructive to the long-term
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                            consulting the information, we found that there may be the following reasons: first of all,
                             development of our project and contribution to society, but also useful to the spread of
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                             the most common chemical hair dyes on the market will cause considerable harm to the human
                             science in the whole society with our own strength.</p>
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                             body; secondly, the domestic public may have a certain degree of prejudice against the
                         <p>We tend to change the society's attitude towards hair dyeing and perming via organizing Brave
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                             exaggerated styling; in addition, the potential value of the plant hair dye market is great,
                             Color Challenge in worldwide. And the widespread of our experience and knowledge is deepened
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                            but the market share is very low.</p>
                             through education in all age. At the same time, our team give out our own strength through
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                         <p>In order to solve the problems above, our team decided to develop a healthy, humanized and
                             social volunteering. Additionally, we develop our product into a complete business plan
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                             reversible integrated ironing and dyeing product. While solving the problems, the plant dyes
                             which software and hardware are included. We also shared our software design on the internet
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                            we choose have also achieved an environmentally friendly breakthrough and contributed to the
                            via open source.</p>
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                             world's environmental protection.</p>
                         <h1>1 Brave color challenge -- attention to social prejudice</h1>
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                        <p>In order to confirm our cause analysis and extensively investigate the opinions of the
                         <div class="image"><img alt="Brave color challenge"
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                             public, we have conducted extensive surveys on people of different identities, consumers,
                                 src="https://static.igem.org/mediawiki/2021/2/20/T--HUST-China--img--humanpractice--HP-4.1.1.png"
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                             entrepreneurs and relevant stakeholders by means of interviews, questionnaires and so on.
                                 style="width: 90%" />
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                        </p>
                             <p>Figure 1: Brave color challenge</p>
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                         <h1>1 Interview</h1>
 +
                        <p>In order to explore consumers' concerns about the health hazards of chemical dyes and
 +
                            people's attitudes towards perming and dyeing, we selected a number of people of different
 +
                            ages and occupations to conduct interviews</p>
 +
                        <h2>1.1 Health Concerns</h2>
 +
                        <h3>Young people</h3>
 +
                         <div class="image"><img alt="Young people"
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                                 src="https://static.igem.org/mediawiki/2021/2/22/T--HUST-China--img--humanpractice--HP-1.1.1.1.png"
 +
                                 style="width: 100%" />
 +
                             <p>Figure 1: Young people</p>
 
                         </div>
 
                         </div>
                         <p>At the beginning of the project, we carried out a wide range of surveys via interviews,
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                         <p>Chemical dyes do great harm to our hair, but nowadays many college students know the harm,
                             questionnaires to inquire consumers, entrepreneurs, stakeholders and different identities,
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                             but still choose to dye their hair. Today, college students seem to have become the main
                            and found that the domestic people may have a certain degree of bias on the style of the
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                             consumer group in the hair dyeing market. For this reason, we interviewed several college
                             exaggerated permed or dyed hair. Therefore, we designed a reversible bio-perm dyeing system
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                             students. Through our interviews and investigations, we found that most college students are
                             that fades instantly, and selected plant dyes to mitigate its harm to health and
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                             very aware of the harm caused by hair dyeing, and they are also very worried about it. After
                            environment. However, during project surveys and field visits, we found that while most
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                             the interview, we found that some people who use chemical dyes to dye their hair more or
                             people were interested in Mr. Tony's products with some trust and expectation, the
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                             less have hair health problems, such as poorer hair quality, hair bifurcations and so on.
                            designated product didn't actually eliminate people's biases.</p>
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                             Frequent use of chemical dyes leads to the deterioration of hair quality. The sequelae of
                        <p>We looked up the relevant information comprehensively and found that prejudice is essentially
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                            appearance reduction such as hair loss, hair loss and bifurcation are the main reasons for
                            a negative understanding and attitude to people and things that bulit up from objective
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                             college students to give up dyeing their hair. Most students who have dyed their hair or
                            facts, just like stereotypes. Prejudice is not easy to be eliminated in a short period of
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                             want to dye their hair say they will choose not to dye their hair if the dye makes their
                            time, and it often requires years of subtle influence to do so. But we wouldn't give up just
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                             hair worse. For natural plant hair dyeing products, most students are very willing to try
                             because it's time-consuming, and HUST-China decided to do its best to do its part in the
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                             and accept, but a small number of students say that they doubt whether natural pigment hair
                            field of perming and dyeing to eliminate prejudice.</p>
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                             dyeing can have the advantages of various color selectivity and high color fastness like
                        <p>In addition, we also found that the most terrible prejudice is often the prejudice against
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                            chemical reagents. Some students said that the price of natural plant hair dyeing product is
                            themselves. Long-term Influenced under growth and living environment, many people become
+
                             also the prime consideration of their choice of dye. College students who do not have a
                             suspicious of themselves after being prejudiced by others. Susan Bealey, M.D. of the
+
                             fixed income may have no choice but to choose relatively cheap chemical dyes.</p>
                            University of British Columbia in Canada and health coach, says it's not uncommon, or even
+
                            quite normal, for perfect and capable people to think they can't. These "no" misconceptions
+
                             are biases that are embedded by others, often from families where people grew up, and may be
+
                            judged from a conservative perspective by family members. And the elimination of this
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                            prejudice often rely on self-contained self-confidence and a strong heart, telling
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                            themselves to be brave and not care about what others think. It is in this way that
+
                            HUST-China launched the Brave Color Challenge around the world, calling on people to
+
                            eliminate prejudice and be brave enough to be themselves.</p>
+
                        <p>The slogan of Brave Color Challenge is to be brave to find the own self, and draws on the
+
                             relay model of the Ice Bucket Challenge to expand the project's impact. We allow challengers
+
                             to change their hair color via Photoshop, eliminating challengers' concerns about hair
+
                             health and social attitudes, skipping tedious thoughts and selection processes, and just
+
                            appreciating their hairstyle after dyeing their hair. We not only provided hair color advice
+
                            for the challenger, more importantly, we aimed to let the challenger find a more beautiful
+
                             self, and encourage them to be brave take steps to embrace their own identity. After the
+
                            challenge, many challengers told us that they did challenge themselves and found
+
                             self-confidence. HUST-China is proud to contribute to the elimination of self-prejudice.</p>
+
                        <p>In addition, we have made the benefits of natural dyes widely understood to challengers and
+
                             the public through knowledge cards in the hope of enhancing public understanding of this
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                             potentially large but low market share industry.</p>
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                                            <p>Interviewees: three college students from Huazhong University of Science
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                                                and Technology</p>
 +
                                            <p>Detailed interview notes are as follows:</p>
 +
                                            <p>Interviewee A:girl with her hair dyed</p>
 +
                                            <p>Q:How long have you dyed your hair? And how many times have you dyed your
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                                                hair?</p>
 +
                                            <p>A:It has been dyed for a while, about a month or so. This is the fourth
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                                                time I have dyed my hair.</p>
 +
                                            <p>Q:The fourth time to dye your hair, it should be regarded as more
 +
                                                relative to dyeing your hair, so what aspects do you value in your
 +
                                                choice of hair dye all the time? Are there any requirements for the
 +
                                                choice of hair dye?</p>
 +
                                            <p>A:What I value more is the raw material of hair dye. I generally prefer
 +
                                                the one that does the least harm to my hair. Because I personally like
 +
                                                to dye my hair, I want to try any kind of color. If the hair dye does
 +
                                                too much harm to my hair, my hair health can not support my desire to
 +
                                                dye all kinds of colors.</p>
 +
                                            <p>Q:May I ask whether your hair dye is chemical dye or natural dye?</p>
 +
                                            <p>A:Chemical dye. I am just worried that the natural dye will be dyed black
 +
                                                like Polygonum multiform, and the color richness may not be as much as
 +
                                                that of chemical dye.</p>
 +
                                            <p>Q:Oh, so if there is a "natural dye hair dye" product on the market, and
 +
                                                there are many colors to choose from, would you want to try it?</p>
 +
                                            <p>A:If the price is also pleasant, it would be great, and it would be a
 +
                                                boon for those of us who are addicted to dyeing our hair.</p>
 +
                                            <p>Interviewee B:girl with black hair</p>
 +
                                            <p>Q:Have you ever dyed your hair? Or do you want to dye your hair?</p>
 +
                                            <p>A:I haven't dyed it. I actually want to dye my hair a little bit.</p>
 +
                                            <p>Q: Oh, so what is the reason why you didn't dye your hair?</p>
 +
                                            <p>A: because my hair has not been very good and dry since I was a child,
 +
                                                and it has a little bifurcation. I'm worried that dyeing my hair will
 +
                                                make it worse for my unhealthy hair.</p>
 +
                                            <p>Q: if hair dye is made from natural pigment, would you like to try it?
 +
                                            </p>
 +
                                            <p>A: yes, I would. Would you like to add chemicals if you dye your hair
 +
                                                with natural pigments?</p>
 +
                                            <p>Q: the dye produced by our project does not add chemicals, and it also
 +
                                                has a good effect on hair dyeing.</p>
 +
                                            <p>A: in that case, I'm more than willing to try.</p>
 +
                                            <p>Interviewee C:girl with black hair</p>
 +
                                            <p>Q: Hi, have you ever dyed your hair?</p>
 +
                                            <p>A: dyed it. But it was a long time ago.</p>
 +
                                            <p>Q: then why don't you dye it now?</p>
 +
                                            <p>A: last time I dyed and bleached my hair, which damaged the quality of my
 +
                                                hair a lot and broke a lot of hair. After that, I never dared to try to
 +
                                                dye my hair again.</p>
 +
                                            <p>Q: did you choose the chemical dye for your hair last time?</p>
 +
                                            <p>A: yes, the barber recommended it, but I think it does too much harm to
 +
                                                the hair.</p>
 +
                                            <p>Q: what do you think of natural hair dyeing products? Have you ever
 +
                                                considered using this kind of product to dye your hair?</p>
 +
                                            <p>A: yes, but the price of this kind of product in the barber shop is
 +
                                                really a little high for me as a student, so I am prohibitive. Hair
 +
                                                dyeing is not necessary, it's just a decoration.</p>
 +
                                            <p>Q: what if there is a hair dye product with friendly price, green raw
 +
                                                material and good effect? Do you have the idea to try?</p>
 +
                                            <p>A: if there is such a product, I would want to dye my hair again. As I
 +
                                                just said, hair dyeing is a decoration, although hair dyeing is
 +
                                                optional, but if you dye it to make yourself look good, why not do it?
 +
                                            </p>
 +
                                        </div>
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                                                type="button">Close</button></div>
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                                    </div>
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                                </div>
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                            </div>
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                        </div>
 +
                        <h3>Elderly people</h3>
 +
                        <p>In order to investigate the views of different age groups on hair dyeing and hair care, we
 +
                            interviewed the middle-aged and elderly people by means of online video. Through our
 +
                            interview, we found that more and more middle-aged and elderly people choose to dye their
 +
                            hair because of the increase in gray hair. Most people say that they are very supportive of
 +
                            hair dyeing, which can cover their silver hair and make a person more energetic in
 +
                            appearance, and their mentality will become younger when they see themselves younger. Many
 +
                            people say this is an act of killing two birds with one stone. Through our survey, we also
 +
                            found that people today are under great social pressure and a fast pace of life, resulting
 +
                            in a continuous decline in the age of silver-haired people, and many middle-aged people in
 +
                            their forties and fifties (not really old age) also have a lot of gray hair. This group is
 +
                            also one of the main consumers in the hair dyeing market. Compared with college students,
 +
                            this kind of hair dye consumers are different in their higher consumption and more pursuit
 +
                            of quality and raw materials. For them, the safety and health of dyes are more important.
 +
                            Many people choose dyes with advertising words such as "traditional Chinese medicine",
 +
                            "natural" and "health". They value health more than a variety of colors and cheap prices.
 +
                            Therefore, we believe that middle-aged and elderly group will be the main consumers of
 +
                            natural dyes.</p>
 +
                        <div class="image"><img alt="Elderly people"
 +
                                src="https://static.igem.org/mediawiki/2021/7/7f/T--HUST-China--img--humanpractice--HP-1.1.1.2.png"
 +
                                style="width: 100%" />
 +
                            <p>Figure 2: Elderly people</p>
 +
                        </div>
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                        <div class="modal-btn"><button class="btn btn-warning pull-right"
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                                data-target="#Interviewees-Elderly" data-toggle="modal" type="button">Read More</button>
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                            <div aria-hidden="true" aria-labelledby="Interviewees-ElderlyLabel" class="modal fade"
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                                data-backdrop="static" data-keyboard="false" id="Interviewees-Elderly" tabindex="-1">
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                                <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
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                                    <div class="modal-content">
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                                        <div class="modal-header">
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                                            <h5 class="modal-title" id="Interviewees-ElderlyLabel">Interviewees-Elderly
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                                            </h5>
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                                        </div>
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                                        <div class="modal-body">
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                                            <p>Interviewees: two middle-aged and elderly residents: two middle-aged and
 +
                                                elderly residents. Detailed interview notes are as follows:</p>
 +
                                            <p>Interviewee D:An old man with black hair mixed with a little gray hair
 +
                                            </p>
 +
                                            <p>Q: what do you think of hair dyeing? Do you want to dye it black when you
 +
                                                have gray hair?</p>
 +
                                            <p>A: I kind of want to dye my hair. Now that white hair grows out, it shows
 +
                                                that people are old, and people with dyed hair look younger and look
 +
                                                better.</p>
 +
                                            <p>Q: so what kind of hair dyeing products will you choose if you dye your
 +
                                                hair later?</p>
 +
                                            <p>A: I see the kind of traditional Chinese medicine dye hair in the
 +
                                                advertisement, which attracts me very much.</p>
 +
                                            <p>Q: that's natural dyes. What do you think of chemical dyes?</p>
 +
                                            <p>A: I always feel that chemical dyes are bad for our health. That hair is
 +
                                                attached to the scalp. It would be bad if some chemical seeps into the
 +
                                                body from the scalp and does any harm to the body. We are old now, and
 +
                                                we are not as good as we used to be. We dare not use it
 +
                                                indiscriminately.</p>
 +
                                            <p>Interviewee E:A dark-haired aunt around 60</p>
 +
                                            <p>Q: Hello, auntie. Your bright black hair is very beautiful. I'd like to
 +
                                                interview you about how to take care of your hair every day.</p>
 +
                                            <p>A: I dyed my hair at home. I'm old now. My hair is gray a lot. I look
 +
                                                young when I dye my hair, and I'm in better spirits.</p>
 +
                                            <p>Q: Oh, what kind of hair dye do you choose to dye your own hair?</p>
 +
                                            <p>A: I saw a traditional Chinese medicine hair dye on TV. It advertised
 +
                                                that traditional Chinese medicine hair dye would not hurt my health. I
 +
                                                bought it to try it.</p>
 +
                                            <p>Q: so you chose this product because its slogan is "traditional Chinese
 +
                                                medicine" and "it doesn't hurt your body"?</p>
 +
                                            <p>A: that's right. We all know traditional Chinese medicine. Recuperating
 +
                                                the body will definitely do much less harm to the scalp.</p>
 +
                                        </div>
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                                        <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
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                                                type="button">Close</button></div>
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                                    </div>
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                                </div>
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                            </div>
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                        </div>
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                        <h2>1.2 Social Attitude</h2>
 +
                        <p>In addition to the health effects of dye reagents, the public's attitude towards hair dyeing
 +
                            is also an important factor restricting the freedom to perm and dye hair. In China,
 +
                            especially among the older generation, perming and dyeing hair, especially those with
 +
                            exaggerated shapes and colors, are often regarded as informal or too lively. Considering the
 +
                            universal practicality of the product, we investigated the attitudes of different classes
 +
                            towards hair dyeing. The overall survey results show that most people are not resistant to
 +
                            hair dyeing, and many people say they want to try. The main problem is that if you want to
 +
                            dye your hair, you need to dye it back, which not only do harms the quality of your hair,
 +
                            but also makes it more troublesome. Therefore, we imagine that the "fade" function will be
 +
                            added to the project, and the color can be faded by using other substances after dyeing the
 +
                            hair.</p>
 +
                        <h3>Company and employees</h3>
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                        <div class="image"><img alt="Company-and-employees"
 +
                                src="https://static.igem.org/mediawiki/2021/7/76/T--HUST-China--img--humanpractice--HP-1.1.2.1.png"
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                                style="width: 100%" />
 +
                            <p>Figure 3: Company-and-employees</p>
 +
                        </div>
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                        <p>For individuals, dyeing hair shows personality, represents avantgarde and their attention
 +
                            towards appearance. But for employees at work, they may be tied up in order to maintain
 +
                            their appearance at a critical moment. For companies, employees should be loyal to their
 +
                            work when they are at work. If there are more such employees, it will increase unnecessary
 +
                            economic and time costs for the company. The boss said that enterprises need to have
 +
                            enterprise culture, regulations, and some restrictions. Company needs to regulate employees'
 +
                            behavior and appearance during work in order to maintain the image of the company. At the
 +
                            same time, the boss also said that apart from working hours, the company has no objection
 +
                            but respect all ideas of employees to manage their appearance and publicize their
 +
                            personality. When interviewing employees, we come to the conclusion that although most
 +
                            people have the desire to dye their hair, it is inconvenient or troublesome for them to go
 +
                            to work. Although the company does not restrict the behavior of individuals during their
 +
                            non-working hours, dyeing their hair after work and back to work for a day or two is both
 +
                            time-consuming, and it will do more harm than good for people who work in society. The
 +
                            person in charge said that he very supports the idea that employees have such an idea to
 +
                            dress themselves up. Employees do what they want to do without violating the company rules,
 +
                            and keeping a happy mood is also a guarantee for the boss</p>
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                                 More</button>
 
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                             <div aria-hidden="true" aria-labelledby="Interviewees-CompanyAndEmployeesLabel"
                                data-backdrop="static" data-keyboard="false" id="Brave-color-challenge" tabindex="-1">
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                                class="modal fade" data-backdrop="static" data-keyboard="false"
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                                id="Interviewees-CompanyAndEmployees" tabindex="-1">
 
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                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                     <div class="modal-content">
 
                                     <div class="modal-content">
 
                                         <div class="modal-header">
 
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                                             <h5 class="modal-title" id="Brave-color-challengeLabel">
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                                             <h5 class="modal-title" id="Interviewees-CompanyAndEmployeesLabel">
                                                 Brave-color-challenge</h5>
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                                                 Interviewees-CompanyAndEmployees</h5>
 
                                         </div>
 
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                                         <div class="modal-body">
 
                                         <div class="modal-body">
                                             <p>Challenge process: The Brave color Challenge draws on the relay model of
+
                                             <p>Interviewees: bosses and employees of a biotechnology company in
                                                 the Ice Bucket Challenge to expand the project's impact by naming the
+
                                                 Hangzhou.</p>
                                                next challenger.</p>
+
                                             <p>Detailed interview notes are as follows:</p>
                                             <p>The Challenge process comes below:</p>
+
                                             <p>Q: (ask the boss) what do you think of employees' hair dyeing? Do you
                                             <p>1 Select the hair color for the next challenger via our self-constructed
+
                                                have any suggestions or ideas on the concept of designing hair dyeing
                                                 program (For detailed information , please refer to software part),</p>
+
                                                 products?</p>
                                             <p>2 The Challenger would use Photoshop or other image-fixing software to
+
                                             <p>A: according to our company's policy, you can't dye that bright color
                                                 change the hair color of their selfie to the color which the previous
+
                                                during working hours. Of course, black and brown are allowed. In view of
                                                 challenger have chosen, referring to the guidance provided by us</p>
+
                                                the nature of some positions, such as customer service, public
                                            <p>3 Challenger would post her selfie before and after recoloring and the
+
                                                relations, outreach and other positions that need to represent the image
                                                 hair color selected for the next challenger on the social website??and
+
                                                 of the company, the company is not allowed to dye hair. Employees go out
                                                 @the next challenger.</p>
+
                                                to work, represents the image of the company, dyed a variety of hair,
                                             <div class="image"><img alt="Brave color challenge"
+
                                                 inevitably will not make customers feel a little immature, do not rest
                                                    src="https://static.igem.org/mediawiki/2021/f/f4/T--HUST-China--img--humanpractice--HP-4.1.2.png"
+
                                                assured to leave the project to us to do. But after work time, we have
                                                    style="width: 40%" />
+
                                                 no rules for the personal behavior of employees, employees can do what
                                                 <p>Figure 2: Brave color challenge</p>
+
                                                they want to do, dye the color they want. If there is a product that can
                                            </div>
+
                                                be dyed and faded at any time, it should be able to solve this big
                                             <p><b>Interview with Challengers:</b>After challenge, we have also interviewed
+
                                                 problem.</p>
                                                 a number of challengers, who said that they did have some bias and
+
                                             <p>Q: (ask employees) what do you think of the company's regulations on hair
                                                trouble about hair dyeing. Through this challenge, they found their
+
                                                dyeing? will hair dyeing really give up for salaried people like you
                                                 adoring hair dye color, and they would also try to use less harmful
+
                                                 because of trouble?</p>
                                                 natural perm and dye to explore their more beautiful image. After we
+
                                             <p>A: I don't think there's anything wrong with this rule. Too conspicuous
                                                introduced our projects to challengers, they really look forward to the
+
                                                 hair color will not only disturb the attention of me and my colleagues,
                                                 reversible and healthy feature of our products.</p>
+
                                                 but may also make a bad impression on customers when they go out to meet
                                            <p><b>Knowledge Cards:</b>We launched the challenge poster and added the design
+
                                                 customers. Although it can be dyed after work, it is really troublesome
                                                of the knowledge card afterwards to promote the challenge while
+
                                                 for us. I am very tired at the end of the day. It will take me a few
                                                 spreading the health benefits of natural dyes to the public. In
+
                                                 hours to dye my hair for two days. It's going to be dyed back on Sunday
                                                 addition, we call on the public to pay attention to the importance of
+
                                                 night. Even if I'm not tired, the hair dye on the market is uneven, and
                                                 environmental protection.</p>
+
                                                 my hair can't stand it.</p>
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
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                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <h1>2 Education</h1>
+
                         <h3>Teachers and students</h3>
                        <p>Education and public outreach are important guidelines for our team. In the past year, we
+
                         <div class="image"><img alt="Teachers-and-students"
                            have carried out a series of educational practices mainly aimed at students. In the process
+
                                 src="https://static.igem.org/mediawiki/2021/7/76/T--HUST-China--img--humanpractice--HP-1.1.2.1.png"
                            of communicating with many schools and institutions, we took the advantages of university
+
                                 style="width: 100%" />
                            platform, and conveyed the team's name and knowledge about synthetic biology to a wider
+
                             <p>Figure 4: Teachers-and-students</p>
                            audience. Our audience mainly includes pupils, secondary school students, college students
+
                            and those who love life science. Our educational activities not only include traditional
+
                            offline knowledge lectures, communication meetings and road shows, but also offer courses in
+
                            popular online media, providing online education resources for public, which is a highlight
+
                            for our team.</p>
+
                        <h3>Primary School</h3>
+
                         <div class="image"><img alt="Primary School"
+
                                 src="https://static.igem.org/mediawiki/2021/1/18/T--HUST-China--img--humanpractice--HP-4.2.1.png"
+
                                 style="width: 90%" />
+
                             <p>Figure 3: Primary School</p>
+
 
                         </div>
 
                         </div>
                         <p>We always believe that the spread of science will not be limited by age. As the first stop of
+
                         <p>We interviewed a student and his counselor teacher from Huazhong University of Science and
                            our education program, we came to the Primary School affiliated to Huazhong University of
+
                            Technology. The feedback is that the teacher has no objection to hair dyeing. However, he
                            Science and Technology. Together with other scientific research teams and individuals of
+
                             believes that when a student is still a student at school, his main mind should be on study.
                             Huazhong University of Science and Technology, we have carried out a series of interesting
+
                            There is still a lot of opposition to hair dyeing during class. Holidays, winter and summer
                             science courses called "Dream Class" in the hope that pupils can establish their dreams in
+
                             holidays, such holiday time, students can do whatever he likes, including hair dyeing and
                             class. In this course, we designed an interesting biology Mini Game to tell the children
+
                            perming. Students dye and perm their hair is their own choice, if he can feel happy from it,
                             about the basic and interesting biology knowledge, and also introduced the past projects of
+
                             the external improvement will also increase self-confidence. For the students, we
                             the HUST-China team to the children in popular language. Unexpectedly, the pupils not only
+
                            interviewed him separately and learned that his idea was similar to that of the counselor.
                             understood the story, but also made wild suggestions to our project.</p>
+
                             When he's at school, he focuses on his studies and doesn't care about it at all. In
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Primary-School"
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                            addition, too conspicuous hair color, also reflects the individual personality publicity,
                                data-toggle="modal" type="button">Read More</button>
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                             may attract more attention, but the student's personality is quiet, he chose to give up hair
                             <div aria-hidden="true" aria-labelledby="Primary-SchoolLabel" class="modal fade"
+
                            color to maintain his serenity. May be during the holiday, a whim will want to try some new
                                data-backdrop="static" data-keyboard="false" id="Primary-School" tabindex="-1">
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                            hair color, change the mental outlook. As a result, we are more certain that the market
 +
                             acceptance level of the "fading" design will be very high.</p>
 +
                         <div class="modal-btn"><button class="btn btn-warning pull-right"
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                                data-target="#Interviewees-Teachers-students" data-toggle="modal" type="button">Read
 +
                                More</button>
 +
                             <div aria-hidden="true" aria-labelledby="Interviewees-Teachers-studentsLabel"
 +
                                class="modal fade" data-backdrop="static" data-keyboard="false"
 +
                                id="Interviewees-Teachers-students" tabindex="-1">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                     <div class="modal-content">
 
                                     <div class="modal-content">
 
                                         <div class="modal-header">
 
                                         <div class="modal-header">
                                             <h5 class="modal-title" id="Primary-SchoolLabel">Primary-School</h5>
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                                             <h5 class="modal-title" id="Interviewees-Teachers-studentsLabel">
 +
                                                Interviewees-Teachers-students</h5>
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                                        </div>
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                                        <div class="modal-body">
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                                            <p>Interview object: teachers and students of Huazhong University of Science
 +
                                                and Technology.</p>
 +
                                            <p>Detailed interview notes are as follows:</p>
 +
                                            <p>Teacher: our school has no objection to students dyeing their hair, but
 +
                                                it does not support students to dye their hair too brightly at school.
 +
                                                After all, a student is a student, and study should be the main thing on
 +
                                                campus. It is a good thing to care about the image, but the image of too
 +
                                                much publicity and personality may distract the students' attention in
 +
                                                school and affect the efficiency of the classroom, so we do not advocate
 +
                                                it.</p>
 +
                                            <p>Student: I think I want to try some different hair colors, but my
 +
                                                personality is not so obvious that I don't want to be too conspicuous at
 +
                                                school. Secondly, I think it is feasible to dye your hair on holiday
 +
                                                weekends if you can give priority to study at school. If there is such a
 +
                                                product, it is really convenient for me to dye it after school and fade
 +
                                                it at the beginning of school.</p>
 +
                                        </div>
 +
                                        <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
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                                                type="button">Close</button></div>
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                                    </div>
 +
                                </div>
 +
                            </div>
 +
                        </div>
 +
                        <h3>Family.</h3>
 +
                        <p>We came to the houses of several students and had an in-depth understanding of what parents
 +
                            think of hair dyeing. A small number of parents said that they could not accept their
 +
                            children to dye their hair because they would think that their children were not paying
 +
                            attention to their studies. But most parents show respect for their children's choice to dye
 +
                            their hair. They say that times are changing, external changes will not affect internal
 +
                            changes, hair dyeing is only a way to pursue fashion personality, they do not want their
 +
                            children to leave regrets on the way to grow up. After investigation, we found that most
 +
                            parents have something in common, that is, children are required not to dye their hair
 +
                            during school. Other parents say that hair dyeing is more harmful to their hair and do not
 +
                            want their children to try. However, after learning about our faded product concept, they
 +
                            expressed their interest. Such healthy and convenient products can not only allay the
 +
                            worries of parents, but also satisfy the will of children to show personality and pursue
 +
                            themselves.</p>
 +
                        <div class="modal-btn"><button class="btn btn-warning pull-right"
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                                data-target="#Interviewees-Family" data-toggle="modal" type="button">Read More</button>
 +
                            <div aria-hidden="true" aria-labelledby="Interviewees-FamilyLabel" class="modal fade"
 +
                                data-backdrop="static" data-keyboard="false" id="Interviewees-Family" tabindex="-1">
 +
                                <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
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                                    <div class="modal-content">
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                                        <div class="modal-header">
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                                            <h5 class="modal-title" id="Interviewees-FamilyLabel">Interviewees-Family
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                                            </h5>
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-body">
 
                                         <div class="modal-body">
                                             <p>Taking into account the interests of primary school students, we chose to
+
                                             <p>Detailed interview notes are as follows:</p>
                                                start with interesting videos of DNA and Mini Game, while carrying on
+
                                            <p>Parent A: I don't agree with children dyeing their hair. Hair dyeing is
                                                 Mini Game, let the children imperceptibly understand the double helix
+
                                                 not only harmful to the hair. And she is still a student, too flamboyant
                                                 structure of DNA, which laid the foundation for the later interest
+
                                                 color that is not good-looking has an impact on learning.</p>
                                                 development. Then, on the basis of video, we explained the structure of
+
                                            <p>Parent B: I have no objection to her child dyeing her hair. She can do
                                                 DNA and other basic and interesting biological knowledge to primary
+
                                                 whatever she wants. It's just that everything needs to be based on the
                                                school students in an easy-to-understand language, hoping to increase
+
                                                 fact that the school is not allowed to dye hair while in school, so hair
                                                 their interest in biology. Next, we tried to tell stories about the past
+
                                                 dyeing is not allowed in school. But during the holidays and weekends,
                                                 projects of the HUST-China team, and used the project as an example to
+
                                                 she can do what she wants.</p>
                                                 give them a more concrete understanding of what synthetic biology can
+
                                            <p>Parent C: I have no objection. Now that the child is an adult, it is his
                                                do. Although it was a little difficult, the children listened to the
+
                                                 freedom to do what he wants. But I learned that hair dyeing is both
                                                 whole project very carefully and unexpectedly understood most of it. In
+
                                                 troublesome and unhealthy, and I'm still worried. The product you just
                                                 the final communication session, the pupils not only asked questions,
+
                                                 mentioned, natural pigment hair dye, can also be faded, this design
                                                 but also freely put forward suggestions for our project.</p>
+
                                                 concept is very good. I think many parents will also support it.</p>
 
                                         </div>
 
                                         </div>
 
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                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <h3>High School</h3>
+
                        <h1>2 Questionnaire</h1>
                         <div class="image"><img alt="High School"
+
                        <p>For thousands of years, people persist in aspiring after beauty, while beauty can't exist
                                 src="https://static.igem.org/mediawiki/2021/6/6e/T--HUST-China--img--humanpractice--HP-4.2.2.png"
+
                            without people. Hair perming and dyeing had been a trend of the world since 4000 B.C..
                                 style="width: 90%" />
+
                            Nowadays, though we could see a blooming hairdressing market of over $380 billion, glance
                             <p>Figure 4: High School</p>
+
                            into the deep-seated requirement of the market is needed. To have a clearer sight of this,
 +
                            we initiate a public survey about perming and dyeing and our idea of them.</p>
 +
                         <h3>Sample Status</h3>
 +
                        <p>Male : Female = 4 : 7.</p>
 +
                        <p>Most around 18-30 and above 45.</p>
 +
                        <p>50% have job under serious circumstances.</p>
 +
                         <div class="image"><img alt="From left to right: Gender, Age, Vocation"
 +
                                 src="https://static.igem.org/mediawiki/2021/4/41/T--HUST-China--img--humanpractice--HP-1.2.1.png"
 +
                                 style="width: 100%" />
 +
                             <p>Figure 5: From left to right: Gender, Age, Vocation</p>
 
                         </div>
 
                         </div>
                        <p>The middle school period is a critical spot for determining personal professional
+
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Sample-Status"
                            preferences. In order to increase the influence of iGEM and popularize the knowledge of
+
                            synthetic biology, our team organized a number of educational activities for middle school
+
                            students. This educational activity covers middle school students of different backgrounds.
+
                            With the vivid content of the course, we rooted curiosity of biology in middle school
+
                            students' hearts.</p>
+
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#High-School"
+
 
                                 data-toggle="modal" type="button">Read More</button>
 
                                 data-toggle="modal" type="button">Read More</button>
                             <div aria-hidden="true" aria-labelledby="High-SchoolLabel" class="modal fade"
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                             <div aria-hidden="true" aria-labelledby="Sample-StatusLabel" class="modal fade"
                                 data-backdrop="static" data-keyboard="false" id="High-School" tabindex="-1">
+
                                 data-backdrop="static" data-keyboard="false" id="Sample-Status" tabindex="-1">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                     <div class="modal-content">
 
                                     <div class="modal-content">
 
                                         <div class="modal-header">
 
                                         <div class="modal-header">
                                             <h5 class="modal-title" id="High-SchoolLabel">High-School</h5>
+
                                             <h5 class="modal-title" id="Sample-StatusLabel">Sample-Status</h5>
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-body">
 
                                         <div class="modal-body">
                                             <p>According to our location advantage, we conducted synthetic biology
+
                                             <the>target population of perming and dyeing consisted of two parts: A. the
                                                lectures for students from two middle schools in Central China. Most of
+
                                                 elders who need their hair kept in black and B. the youngsters who want
                                                 the students who participated in the lectures have knowledge backgrounds
+
                                                 to display their own personality. So, we have our informants mainly
                                                 in life science competitions, so in the presentation, we focus on
+
                                                 under 18 and above 40. Furthermore, more than half of the informants go
                                                broadening the knowledge of students . In the lecture, we popularized
+
                                                 in for vocations that have strict unwritten rules about their outfit
                                                 the basic concepts of synthetic biology, and from the perspective of
+
                                                 being dignified, such as students, teachers and government officers.
                                                practical application, we led the students to further understand the
+
                                             </the>
                                                knowledge of molecular biology, biochemistry and metabolomics. In the
+
                                                lecture, we briefly talked about the content of the iGEM competition,
+
                                                 and also introduced the HUST-China team.</p>
+
                                            <p>Taking this as an opportunity, we introduced the team's projects in the
+
                                                 past two years, led the students to review the conception process of our
+
                                                project, and shared our project creativity and some parts of the project
+
                                                design.</p>
+
                                             <p>After the lecture, we had a heated discussion with the students on the
+
                                                content just described.While answering questions, we also expressed the
+
                                                hope that these high school students who love biology can pay attention
+
                                                to synthetic biology and be able to apply it to real-world problems.</p>
+
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
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                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <h3>Universities</h3>
+
                         <h3>Did You Dare?!</h3>
                         <div class="image"><img alt="Universities"
+
                        <p>Most did have perming and dyeing but mostly through the chemical method, and we all know what
                                 src="https://static.igem.org/mediawiki/2021/e/e0/T--HUST-China--img--humanpractice--HP-4.2.3.png"
+
                            will happen when it comes to 'chemical' in human body.</p>
                                 style="width: 90%" />
+
                         <div class="image"><img
                             <p>Figure 5: Universities</p>
+
                                alt="From left to right: Have you ever had a perm?, Have you dyed your hair before?"
 +
                                 src="https://static.igem.org/mediawiki/2021/5/51/T--HUST-China--img--humanpractice--HP-1.2.2.png"
 +
                                 style="width: 100%" />
 +
                             <p>Figure 6: From left to right: Have you ever had a perm?, Have you dyed your hair before?
 +
                            </p>
 
                         </div>
 
                         </div>
                         <p>The university itself is a diversified learning platform, for our university students to
+
                         <div class="image"><img
                            popularize science provides a lot of convenience. In the education of college students, we
+
                                alt="From left to right: How did you have your perm? What kind of dye did you use?"
                            rely on the institution of the school to show them synthetic biology and iGEM in a more
+
                                src="https://static.igem.org/mediawiki/2021/c/cf/T--HUST-China--img--humanpractice--HP-1.2.3.png"
                            official and professional image. At the same time, we also attach importance to the training
+
                                style="width: 100%" />
                            of new team members. Since the beginning of this year, we have inherited the spirit and
+
                            <p>Figure 7: From left to right: How did you have your perm? What kind of dye did you use?
                            tradition of iGEM predecessors, and cultivated a group of brand new iGEM team members. In
+
                             </p>
                            addition, we also actively publicized our humanistic practice overview and gains to the
+
                        </div>
                            public, so that more people could see how large-scale practical activities are
+
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Did-You-Dare"
                            scientifically organized and carried out.</p>
+
                        <p>HUST-China team has undertaken part of the "Qiming Courses" to enable students of many other
+
                            majors to understand synthetic biology-related knowledge, as long as hair dyeing experience
+
                             activities.</p>
+
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Universities"
+
 
                                 data-toggle="modal" type="button">Read More</button>
 
                                 data-toggle="modal" type="button">Read More</button>
                             <div aria-hidden="true" aria-labelledby="UniversitiesLabel" class="modal fade"
+
                             <div aria-hidden="true" aria-labelledby="Did-You-DareLabel" class="modal fade"
                                 data-backdrop="static" data-keyboard="false" id="Universities" tabindex="-1">
+
                                 data-backdrop="static" data-keyboard="false" id="Did-You-Dare" tabindex="-1">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                     <div class="modal-content">
 
                                     <div class="modal-content">
 
                                         <div class="modal-header">
 
                                         <div class="modal-header">
                                             <h5 class="modal-title" id="UniversitiesLabel">Universities</h5>
+
                                             <h5 class="modal-title" id="Did-You-DareLabel">Did-You-Dare</h5>
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-body">
 
                                         <div class="modal-body">
                                             <p>Qiming College is an institution established by Huazhong University of
+
                                             <p>For the start, we would like to figure out whether the informants had
                                                Science and Technology (HUST) to cultivate top-notch innovative talents.
+
                                                 experience of perming and dyeing-and why. As a result, we find out that
                                                Qiming College has launched a series of "Qiming Courses" as the
+
                                                 about 70% of them had experience of perming and 60% who comes to dyeing.
                                                interdisciplinary learning platform. Through communication with college,
+
                                                 For those who have the experience of perming, potion perm (92.13%) is
                                                we applied for the qualification to set up the course.</p>
+
                                                 far more famous than heated roller ones (18.45%). For those who have the
                                            <p>As a representative of basic subject education, the HUST-China team has
+
                                                 experience of dyeing, chemical dye (70%) beats plant-based dye (27%).
                                                 undertaken part of the "Qiming Courses" to enable students of many other
+
                                                majors to understand synthetic biology-related knowledge. Around
+
                                                synthetic biology, we introduced the basic background knowledge of basic
+
                                                 biochemistry and molecular biology.</p>
+
                                            <div class="image"><img
+
                                                    alt="Our team members are talking about biochemistry related knowledge"
+
                                                    src="https://static.igem.org/mediawiki/2021/2/2b/T--HUST-China--img--humanpractice--HP-4.2.4.png"
+
                                                    style="width: 90%" />
+
                                                <p>Figure 6: Our team members are talking about biochemistry related
+
                                                    knowledge</p>
+
                                            </div>
+
                                            <p>In addition, with this platform, we also promoted iGEM: the situation of
+
                                                our team and the basic overview of the 2021 project to students. Closely
+
                                                 related to the content of the project, the students were taught the
+
                                                construction of engineered cells and the scientific knowledge of hair
+
                                                dyeing and perming. In the course, we provided hair dyeing experience
+
                                                 activities. We provided hair and a variety of dyes for the students .
+
                                                Students could DIY their own hair color and styling, so as to have a
+
                                                 deeper understanding of the creativity and feasibility of our project.
+
 
                                             </p>
 
                                             </p>
                                             <div class="image"><img alt="Students DIY their hair styling"
+
                                             <p>Having proved that perming and dyeing is the trend of fashion, we could
                                                    src="https://static.igem.org/mediawiki/2021/1/1d/T--HUST-China--img--humanpractice--HP-4.2.5.png"
+
                                                 still see more 'chemistry' than 'biology' here. When hairdressing
                                                    style="width: 90%" />
+
                                                 measures were born, seldom did people care about what these chemicals
                                                <p>Figure 7: Students DIY their hair styling</p>
+
                                                 had done to people's outer 'organs'of the hair. It is true that whatever
                                            </div>
+
                                                 used on our hair doesn't participate in our metabolism, but they will
                                            <div class="image"><img alt="Student is sharing his work"
+
                                                 eventually touch the scalp, which seems inevitable whatsoever.</p>
                                                    src="https://static.igem.org/mediawiki/2021/8/80/T--HUST-China--img--humanpractice--HP-4.2.6.png"
+
                                                    style="width: 90%" />
+
                                                <p>Figure 8: Student is sharing his work</p>
+
                                            </div>
+
                                            <p>In other Qiming courses, we specially offered courses related to
+
                                                 tie-dyeing craftsmanship, teaching students to dye handicrafts with
+
                                                 natural pigments. At the same time, taking this as an opportunity, we
+
                                                 highlighted the significance of the engineered cells constructed by our
+
                                                 project, and demonstrated the environmental protection and health of
+
                                                 green and natural pigment synthesis. With this course, we once took the
+
                                                concept of the project deeply into the hearts of the students and
+
                                                greatly promoted the creativity of the team.</p>
+
                                            <div class="image"><img alt="Students show the crafts they made in class"
+
                                                    src="https://static.igem.org/mediawiki/2021/9/9b/T--HUST-China--img--humanpractice--HP-4.2.7.png"
+
                                                    style="width: 90%" />
+
                                                <p>Figure 9: Students show the crafts they made in class</p>
+
                                            </div>
+
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
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                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <div class="image"><img alt="In-team education"
+
                        <h3>The Latent Beast</h3>
                                 src="https://static.igem.org/mediawiki/2021/e/e1/T--HUST-China--img--humanpractice--HP-4.2.8.png"
+
                        <p>Most people do concern about the danger behind chemical hairdressing. There is scream of new
                                 style="width: 90%" />
+
                            perming and dyeing method with less 'cost'.</p>
                             <p>Figure 10: In-team education</p>
+
                         <div class="image"><img
 +
                                alt="From left to right: People's know-about of negative effects of perm, People's know-about of negative effects of dyeing"
 +
                                 src="https://static.igem.org/mediawiki/2021/a/a4/T--HUST-China--img--humanpractice--HP-1.2.4.png"
 +
                                 style="width: 100%" />
 +
                             <p>Figure 8: From left to right: People's know-about of negative effects of perm, People's
 +
                                know-about of negative effects of dyeing</p>
 
                         </div>
 
                         </div>
                         <p>Every year we will provide months of in-team education. The coverage of the member education
+
                         <div class="modal-btn"><button class="btn btn-warning pull-right"
                            is very broad, not only includes the necessary theoretical knowledge such as biochemistry,
+
                                data-target="#The-Latent-Beast" data-toggle="modal" type="button">Read More</button>
                             molecular biology and synthetic biology.</p>
+
                            <div aria-hidden="true" aria-labelledby="The-Latent-BeastLabel" class="modal fade"
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#In-team"
+
                                data-backdrop="static" data-keyboard="false" id="The-Latent-Beast" tabindex="-1">
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                                <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
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                                            <h5 class="modal-title" id="The-Latent-BeastLabel">The-Latent-Beast</h5>
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                                            <p>So, do people aware of what kind of ferocious beast come with the
 +
                                                chemical-based material? Well, we do have 10% of warriors who don't pay
 +
                                                any attention to the toxic side effects and 15% of the rest consider all
 +
                                                the perming and dyeing as harmless. But for the majority, people do know
 +
                                                some of the danger lying under the peace. From all these, the conclusion
 +
                                                is clear. With all the awareness of all this and that, people still
 +
                                                choose to 'try their luck'. Since it could change myself for the better
 +
                                                so why not? Indeed, not everyone who have encounter with these chemicals
 +
                                                would get hit badly, but what if we could eliminate all the horrible?
 +
                                            </p>
 +
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 +
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                                </div>
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                            </div>
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                        </div>
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                        <h3>Instant: In and Out, On and Off</h3>
 +
                        <p>Aside from damage, we find out that it is the time cost of getting the hair back to normal
 +
                             that is the main factor blocking people from trying perming and dyeing. Instead of having
 +
                            for days and weeks, what if we could shorten this time window into hours?</p>
 +
                        <div class="image"><img
 +
                                alt="From left to right: Why didn't have a perm?, Why didn't have dyeing?"
 +
                                src="https://static.igem.org/mediawiki/2021/6/65/T--HUST-China--img--humanpractice--HP-1.2.5.png"
 +
                                style="width: 100%" />
 +
                            <p>Figure 9: From left to right: Why didn't have a perm?, Why didn't have dyeing?</p>
 +
                        </div>
 +
                        <div class="image"><img
 +
                                alt="From left to right: Why don't choose potion perm?, Do you need a product of instant hair customizing or reverse reaction with simple operation."
 +
                                src="https://static.igem.org/mediawiki/2021/5/57/T--HUST-China--img--humanpractice--HP-1.2.6.png"
 +
                                style="width: 100%" />
 +
                            <p>Figure 10: From left to right: Why don't choose potion perm?, Do you need a product of
 +
                                instant hair customizing or reverse reaction with simple operation.</p>
 +
                        </div>
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                             <div aria-hidden="true" aria-labelledby="InstantLabel" class="modal fade"
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                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
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                                         <div class="modal-body">
                                             <p>HUST-China has always paid special attention to team spirit inheritance
+
                                             <p>Besides about 40% of our informants who are just not interested, when
                                                and new member training. Every year we will provide months of in-team
+
                                                 asked about why not having their hair customed as they want,
                                                 education. The coverage of the member education is very broad, not only
+
                                                 surprisingly, the primary obstacle isn't the possible side effects (both
                                                includes the necessary theoretical knowledge such as biochemistry,
+
                                                 45% in perm and dyeing). Instead, it's the fear of unsatisfactory result
                                                 molecular biology and synthetic biology. In addition, we timely convey
+
                                                 (27%i in perm and 30% in dyeing) and limitation of daily requirement
                                                the update of our project to new members through weekly meetings, so as
+
                                                 (30% in perming and 37% in dyeing). Meeting one of Mr. Tony's
                                                to ensure that members can keep abreast of the latest situation of the
+
                                                 improvements: instant perming &amp; dyeing. With the minimum damage to
                                                 project. Before the experiment began, experienced team member lead the
+
                                                 the hair, custom your hair colour and convolution degree and have an
                                                new members to the laboratory for half a month of continuous training,
+
                                                 immediate experience, which you could get rid of whatever doesn't work
                                                 which ensured the new team members to master the experimental skills,
+
                                                 for you, and be right where you need to be after showing off who you
                                                avoiding wasting time and mistakes in formal experiments.</p>
+
                                                 really are. It sounds like having multiple customed wig at the same time
                                            <p>The iGEM team members have carried out a variety of practices for the
+
                                                 while you could dominate them any time now. According to the survey, we
                                                 project in their spare time, and the summer practice is the most
+
                                                could see more than three-quarter of our informants is looking forward
                                                 abundant and detailed. We basically introduced the basic outline of our
+
                                                to this type of application.</p>
                                                 practice to the students and teachers, as well as the framework of
+
                                                 several practice processes. During the holidays, our team members went
+
                                                to various parts of China to conduct in-depth and comprehensive
+
                                                 investigations on the current situation of the natural pigment and hair
+
                                                 dye industry. Our practical experience provided a reference template for
+
                                                other scientific research of the same major and different majors. At the
+
                                                 same time, we also publicize the hardship and value of our practice.</p>
+
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
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                         </div>
 
                         </div>
                         <h3>Bilibili open course</h3>
+
                         <h3>Mr. Tony Has a Light Touch and a Long Way to Go</h3>
                         <div class="image"><img alt="Bilibili open course"
+
                        <p>Little damage, instant decolorization, DIY, environmentally friendly... With all these
                                 src="https://static.igem.org/mediawiki/2021/7/77/T--HUST-China--img--humanpractice--HP-4.2.9.png"
+
                            shining points, Mr. Tony still has a lot to conquer.</p>
                                 style="width: 90%" />
+
                         <div class="image"><img
                             <p>Figure 11: Bilibili open course</p>
+
                                alt="From left to right: Have you ever heard of plant-based dye?, Would you choose plant-based dye?"
 +
                                 src="https://static.igem.org/mediawiki/2021/c/c2/T--HUST-China--img--humanpractice--HP-1.2.7.png"
 +
                                 style="width: 100%" />
 +
                             <p>Figure 11: From left to right: Have you ever heard of plant-based dye?, Would you choose
 +
                                plant-based dye?</p>
 
                         </div>
 
                         </div>
                         <p>This year, we innovatively launched online courses. With the current trend of online course
+
                         <div class="image"><img
                            resource learning becoming stronger, we uploaded a series of online courses on the Bilibili
+
                                alt="From left to right: What do you worry about plant-based dye?, What feature attracts you most?, Is there anything else you are worry about?"
                            website, which are very popular among Chinese youth groups. The course content covers a wide
+
                                src="https://static.igem.org/mediawiki/2021/a/ad/T--HUST-China--img--humanpractice--HP-1.2.8.png"
                            range, including not only a variety of basic theoretical knowledge, but also a full range of
+
                                style="width: 100%" />
                             application software teaching, aiming to train the audience to become a qualified iGEM
+
                             <p>Figure 12: From left to right: What do you worry about plant-based dye?, What feature
                            player through the course.</p>
+
                                attracts you most?, Is there anything else you are worry about?</p>
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                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Long-way-to-Go"
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+
                             <div aria-hidden="true" aria-labelledby="Long-way-to-GoLabel" class="modal fade"
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                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
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                                             <h5 class="modal-title" id="Bilibili-open-courseLabel">Bilibili-open-course
+
                                             <h5 class="modal-title" id="Long-way-to-GoLabel">Long-way-to-Go</h5>
                                            </h5>
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                                         </div>
 
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                                         <div class="modal-body">
 
                                         <div class="modal-body">
                                             <p>Affected by the epidemic, we chose to train our team members through
+
                                             <p>Speaking of damage, when it comes to genetically modified organism (GMO),
                                                 online open courses at Bilibili. Our team has selected some excellent
+
                                                 nearly 30% of people turn their concern from the chemical damage to the
                                                players to teach online courses. The contents of the open course include
+
                                                 uncertain compromise of GMOs. A fermenter and a container are designed,
                                                biochemistry, molecular biology, literature retrieval and so on, In
+
                                                 which provide separation of substance from those biologically active
                                                addition, we have also conducted brief training for software such as
+
                                                 things. Since we use bio-product as a substitute for chemical reducer,
                                                PS,AE,snapgene and other software to better participate in the
+
                                                 we attract our customers with minimum damage to the hair (80%) and to
                                                 competition.</p>
+
                                                 the environment (58%). But still, we should not ignore that people
                                            <div class="image"><img alt="Explanation on the use of snapgene"
+
                                                 should be convinced that our product has longer and more stable effect
                                                    src="https://static.igem.org/mediawiki/2021/3/33/T--HUST-China--img--humanpractice--HP-4.2.10.png"
+
                                                 (46%) and is biohazard-free. After all, it takes guts to scrawl
                                                    style="width: 90%" />
+
                                                 something freshly comes from microorganism and culture medium.</p>
                                                 <p>Figure 12: Explanation on the use of snapgene</p>
+
                                            </div>
+
                                            <p>In these courses, we also give a systematic and detailed introduction to
+
                                                 the iGEM competition. Our team members explained in detail the
+
                                                considerations to be considered in designing a project and the steps for
+
                                                project design. In order to better find the genetic information and
+
                                                protein information we need, we have special team members to explain the
+
                                                use and search of databases such as NCBI,KEGG. During the training of
+
                                                 the team members, we made a comprehensive recording of the course,
+
                                                uploaded the video to bilibili, the largest video website in China, and
+
                                                optimized its content, in order to help students master basic literature
+
                                                 retrieval knowledge and skills. the use of some commonly used software,
+
                                                as well as the explanation of some professional knowledge. We hope that
+
                                                through the online open course, we can let you master more and more
+
                                                 comprehensive knowledge, publicize our project with the help of the
+
                                                Internet, at the same time, let more people know about synthetic biology
+
                                                 and IGEM events, cultivate students' interest in synthetic biology and
+
                                                increase their knowledge about it.</p>
+
                                            <div class="image"><img alt="Explanation of experimental principle"
+
                                                    src="https://static.igem.org/mediawiki/2021/3/37/T--HUST-China--img--humanpractice--HP-4.2.11.png"
+
                                                    style="width: 90%" />
+
                                                <p>Figure 13: Explanation of experimental principle</p>
+
                                            </div>
+
                                            <p>Open courses in partnership with UCAS-China IGEM HUST-China offers
+
                                                courses for team members every year, and in order to better inherit and
+
                                                 carry forward the courses, we have recorded some open courses on
+
                                                software and basic knowledge and decided to continue to expand
+
                                                accordingly. However, with the attitude of learning from each other and
+
                                                making progress together, we have decided to work with UCAS-China to
+
                                                continue to expand our courses in more comprehensive areas of knowledge.
+
                                                At present, the plan is in progress.</p>
+
 
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                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <h1>3 Social public welfare and influence</h1>
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                         <h3>The original questionnaire</h3>
                         <h2>Donation of plant dye handicrafts</h2>
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                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Questionnaire"
                        <div class="image"><img alt="Donation of plant dye handicrafts"
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                                data-toggle="modal" type="button">Read More</button>
                                src="https://static.igem.org/mediawiki/2021/c/c7/T--HUST-China--img--humanpractice--HP-4.2.12.png"
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                            <div aria-hidden="true" aria-labelledby="QuestionnaireLabel" class="modal fade"
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                                data-backdrop="static" data-keyboard="false" id="Questionnaire" tabindex="-1">
                            <p>Figure 14: Donation of plant dye handicrafts</p>
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                                            <h5 class="modal-title" id="QuestionnaireLabel">Questionnaire</h5>
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                                        </div>
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                                        <div class="modal-body"><iframe height="500px"
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                                                src="https://static.igem.org/mediawiki/2021/b/b7/T--HUST-China--hp-qnr.pdf" width="100%"></iframe>
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                                        </div>
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                                        <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
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                                                type="button">Close</button></div>
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                                </div>
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                            </div>
 
                         </div>
 
                         </div>
                         <p>In July 2021, we participated in an education-aiding public welfare activity in Lianhua
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                        <h1>3 Key Stakeholder</h1>
                             Village, Pingxiang , Jiangxi Province for 21 days. An investigation about autism in Jiangxi
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                        <h2>3.1 Hair Salon</h2>
                             and several commonweal courses including bandhnu, popularization of synthetic biology, etc
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                         <p>In recent years, consumers' awareness of "green health" is getting stronger and stronger. In
                             were applied to students from Xiaoshui Village, Chetian Village, Shanxia Village and Lianhua
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                             barber shops, people are more inclined to healthy and harmless green products than chemical
                             Village. During the popularization course, a vivid and lucid drama was used to reveal how
+
                            ironing agents that do great damage to their hair. However, in China, the upstream of plant
                             synthetic biology works and benefits the whole society. From the investigation, we found
+
                             perming and dyeing industry is mainly the extraction of various colored plant components,
                             that there was 29.8% of them suffering from severe autism, which was inextricably related to
+
                             dye processing equipment, etc. in the process of plant dye production, the problem of long
                             relationship with parents and friends, while whether their parents were working outside of
+
                            consumption cycle, large input of manpower and material resources and low extraction
                             town had no influence on them. To promote bandhnu and improve the autism and loneliness of
+
                             efficiency cannot be solved well. In the downstream of plant dye industry (like dyeing
                             the students, we established a bandhnu course towards all grades and specialized one of
+
                            barbershop), plant dyes are difficult to fix and fast to decolorize. High cost also
                             them. Colourful and various types of clothes, bags and scarves were created from them and
+
                             discourages domestic barbershop from using plant hair dyes. Therefore, with the
                             displayed in a T show during the farewell party. This public welfare activity expanded their
+
                             diversification of market consumption and the need for the pursuit of health, the
                             horizon, exposed them to professional knowledge and techniques, enhanced their confidence
+
                            traditional plant perming and dyeing process needs to absorb modern scientific knowledge and
                             and did deserve all our effort.</p>
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                             use materialization methods, so as to develop into high-tech fields, improve and enhance its
                         <h2>Popularization of synthetic biology</h2>
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                            own application value. Mr. Tony needs to grasp this trend, to use microbial fermentation to
                         <div class="image"><img alt="Popularization of synthetic biology"
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                            overcome the problem of low extraction efficiency of components in the traditional industry,
                                 src="https://static.igem.org/mediawiki/2021/5/53/T--HUST-China--img--humanpractice--HP-4.2.13.png"
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                             and to reduce the production manpower, material resources and time cost, also explore the
                                 style="width: 90%" />
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                            best coloring conditions of each dye by carrying out relevant experiments. It is also
                             <p>Figure 15: Popularization of synthetic biology</p>
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                             necessary to evaluate the ability of natural dyes to adhere to hair and the effect of
 +
                             natural dyes on hair properties. Pay attention to the decolorization time of dyes while
 +
                             improving color fixation. In the part of hair dyeing, we need to select the perm agent which
 +
                             can easily enter the hair cortex on the basis of the traditional perm principle, so as to
 +
                             meet people's perm requirements healthily without harming the quality of the hair.</p>
 +
                         <h3>3M original barber shop in Jiaozuo , Henan</h3>
 +
                         <div class="image"><img alt="3M original barber shop in Jiaozuo , Henan"
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                                 src="https://static.igem.org/mediawiki/2021/0/09/T--HUST-China--img--humanpractice--HP-1.3.1.1.png"
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                                 style="width: 50%" />
 +
                             <p>Figure 13: 3M original barber shop in Jiaozuo , Henan</p>
 
                         </div>
 
                         </div>
                         <div class="modal-btn"><button class="btn btn-warning pull-right"
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                        <p>Mr.Tony need to reduce the cost of plant hair dyeing and provide customers with long-lasting
                                data-target="#Popularization-of-synthetic-biology" data-toggle="modal"
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                            and healthy hair dyeing effect.</p>
                                type="button">Read More</button>
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                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#mega"
                             <div aria-hidden="true" aria-labelledby="Popularization-of-synthetic-biologyLabel"
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                                data-toggle="modal" type="button">Read More</button>
                                class="modal fade" data-backdrop="static" data-keyboard="false"
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                             <div aria-hidden="true" aria-labelledby="megaLabel" class="modal fade" data-backdrop="static"
                                id="Popularization-of-synthetic-biology" tabindex="-1">
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                                data-keyboard="false" id="mega" tabindex="-1">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
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                                             <h5 class="modal-title" id="Popularization-of-synthetic-biologyLabel">
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                                             <h5 class="modal-title" id="megaLabel">mega</h5>
                                                Popularization-of-synthetic-biology</h5>
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                                         </div>
 
                                         </div>
 
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                                         <div class="modal-body">
                                             <p>On the 'Science' course, we popularize synthetic biology to students from
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                                             <p>In an interview with the barber's owner, we learned that the perming and
                                                 different grades. The indispensable vital role synthetic biology played
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                                                dyeing shop had innovated the perm &amp; dye substance. There are mostly
                                                 in modern life was unfolded with illustration of Covid-19 pandemic.
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                                                chemical perm dyes on the market, which do great damage to the hair. So
                                                 'Drama' courses of interest was also provided, using our project Mr.
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                                                 they aim at the plant hair dye, which is a new type of dye. Because
                                                 Tony as prototype to pose students as different parts - enzymes,
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                                                there are no harmful chemicals that do great damage to the hair, it can
                                                 products, etc. Through which let students comprehend the 'secret' of
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                                                greatly reduce the hair damage caused by perm dyeing. And on the whole,
                                                 synthetic biology.</p>
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                                                the trend of using plant dyes is increasing day by day. After people's
 +
                                                material foundation is satisfied, consumers gradually pursue a healthier
 +
                                                 life, so they are full of confidence in plant hair dyeing. It is
 +
                                                 believed that the demand for plant hair dyes will continue to increase
 +
                                                in the future, but at present, the cost of plant hair dyeing is
 +
                                                generally high and the dyeing effect isn't excellent. Therefore, our Mr.
 +
                                                 Tony needs to solve the problem of high cost of plant hair dyeing, at
 +
                                                 the same time, through exploring biochemical reactions in the hair
 +
                                                 dyeing process and starting from the principle, our product needs to
 +
                                                provide customers with long-lasting and healthy hair dyeing effect, so
 +
                                                as to meet the hairdressing needs of consumers in the future.</p>
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
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Line 594: Line 877:
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <h2>Investigation of autism</h2>
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                         <h3>Plant-based hair dye shop</h3>
                         <div class="image"><img alt="Distribution status of suburb children's autism"
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                         <div class="image"><img alt="Plant-based hair dye shop"
                                 src="https://static.igem.org/mediawiki/2021/8/8e/T--HUST-China--img--humanpractice--HP-4.2.14.png"
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                                 src="https://static.igem.org/mediawiki/2021/1/12/T--HUST-China--img--humanpractice--HP-1.3.1.2.png"
                                 style="width: 90%" />
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                                 style="width: 50%" />
                             <p>Figure 16: Distribution status of suburb children's autism</p>
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                             <p>Figure 14: Plant-based hair dye shop</p>
 
                         </div>
 
                         </div>
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                        <p>Mr. Tony need to comply with contemporary consumers in the pursuit of beauty while paying
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                            attention to the trend of health and solve the problems of high pollution.</p>
 
                         <div class="modal-btn"><button class="btn btn-warning pull-right"
 
                         <div class="modal-btn"><button class="btn btn-warning pull-right"
                                 data-target="#Investigation-of-autism" data-toggle="modal" type="button">Read
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                                 data-target="#Plant-based-hair-dye-shop" data-toggle="modal" type="button">Read
 
                                 More</button>
 
                                 More</button>
                             <div aria-hidden="true" aria-labelledby="Investigation-of-autismLabel" class="modal fade"
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                             <div aria-hidden="true" aria-labelledby="Plant-based-hair-dye-shopLabel" class="modal fade"
                                 data-backdrop="static" data-keyboard="false" id="Investigation-of-autism" tabindex="-1">
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                                 data-backdrop="static" data-keyboard="false" id="Plant-based-hair-dye-shop"
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                                tabindex="-1">
 
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                                             <h5 class="modal-title" id="Investigation-of-autismLabel">
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                                                 Investigation-of-autism</h5>
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                                                 Plant-based-hair-dye-shop</h5>
 
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                                         <div class="modal-body">
                                             <p>Self-evaluation scale: 'Children Solitude Scale' of S. R. Asher, 1984 was
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                                             <p>After communicating with another owner of the barber shop, we learned
                                                 used in the investigation by cluster sampling adopted to students from
+
                                                 that in the upstream of the dye industry, relevant approval of sewage
                                                 grade 3 to 6. Giving out 141 questionnaires, getting feedback from all
+
                                                 disposal needs to be strictly in accordance with the national standards.
                                                 141, 100% of response rate. After eliminating invalid questionnaires
+
                                                 The government has different requirements for different enterprises.
                                                 (too regular the answers or with too much deletion) by surveyors
+
                                                 Each production enterprise needs emission permit and has strict limits
                                                 manually, we've got 131 effective questionnaires, 92.9% of efficiency.
+
                                                 on the pollution factors in the discharge. And now the dye companies on
                                                 The whole process was anonymous and be granted from subjects of their
+
                                                 the market have to pass the test and be allowed to discharge pollution
                                                 own will. Under instruction of surveyors, subjects filled the
+
                                                 after being qualified. At the same time, the production of raw materials
                                                questionnaires on-site. The questionnaires include control point scale
+
                                                 must be in accordance with the standards and indicators issued by the
                                                and age, gender, number of friends, living standard, status of parents'
+
                                                state. In the downstream of the industry, enterprises who need or use
                                                 vocation, and some other demographic indicators.</p>
+
                                                 dye will not be excessively investigated dye raw materials are natural
                                            <p>The result is divided into two parts.</p>
+
                                                 or chemical in the premise of qualified indicators. However, it is found
                                            <p>A. Distribution status of suburb children's autism. We have subjects of
+
                                                 that consumers' awareness of "green and health" is becoming stronger and
                                                 74 male (56.5%) and 57 female (43.5%), average age of 10.34±1.32. 45.8%
+
                                                 stronger, and they are more inclined to healthy and harmless green
                                                 of them seemed to have slight or medium autism while another 29.8%
+
                                                 products. Green dye, dye with Chinese medicine, which show small harm to
                                                 suffering from severe autism. (see Fig1)</p>
+
                                                 body and healthier, are more popular among consumers. In this condition,
                                            <p>Fig1. Distribution status of suburb children's autism</p>
+
                                                Mr. Tony need to solve the problem at the starting point, holding
                                            <div class="image"><img
+
                                                 "health, natural, green" as the goal and trying to use hair cream or
                                                    alt="Distribution status of suburb children's autism"
+
                                                hair dye comb as the carrier to produce excellent products, so as to
                                                    src="https://static.igem.org/mediawiki/2021/8/8e/T--HUST-China--img--humanpractice--HP-4.2.14.png"
+
                                                 comply with contemporary consumers in the pursuit of beauty while paying
                                                    style="width: 90%" />
+
                                                 attention to the trend of health. At the same time, it also needs to be
                                                <p>Figure 17: Distribution status of suburb children's autism</p>
+
                                                 committed to solving the problems of high pollution, high consumption
                                            </div>
+
                                                 and high cost in the upstream of the industry. It is not only harmless
                                            <p>B. Distribution feature of suburb children's autism. We found the
+
                                                to human body, but also friendly to the environment.</p>
                                                 academic status, number of friends and relationship with parents are
+
                                                 related to autism degree (P&lt;0.05) while having nothing to do with
+
                                                 status of parents' working out of town (P&gt;0.05). Less the friends,
+
                                                 higher the score in autism test. Worse the relationship with parents,
+
                                                 worse the autism.</p>
+
                                            <p>Tab2. Distribution feature of suburb children's autism</p>
+
                                            <table>
+
                                                 <thead>
+
                                                    <tr>
+
                                                        <th colspan="2">Demographic indicators</th>
+
                                                        <th>Total</th>
+
                                                        <th colspan="3">Status of autism</th>
+
                                                        <th>F</th>
+
                                                        <th>P</th>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <th></th>
+
                                                        <th></th>
+
                                                        <th>Non-autism</th>
+
                                                        <th>Slight/ Medium</th>
+
                                                        <th>Severe</th>
+
                                                        <th></th>
+
                                                        <th></th>
+
                                                    </tr>
+
                                                 </thead>
+
                                                 <tbody>
+
                                                    <tr>
+
                                                        <td>Academic status</td>
+
                                                        <td>Excellent</td>
+
                                                        <td>27</td>
+
                                                        <td>11(40.7)</td>
+
                                                        <td>11(40.7)</td>
+
                                                        <td>5(18.5)</td>
+
                                                        <td>2</td>
+
                                                        <td>0</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Good</td>
+
                                                        <td>29</td>
+
                                                        <td>8(27.6)</td>
+
                                                        <td>15(51.7)</td>
+
                                                        <td colspan="3">6(20.7)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Medium</td>
+
                                                        <td>45</td>
+
                                                        <td>9(20.0)</td>
+
                                                        <td>23(51.1)</td>
+
                                                        <td colspan="3">13(28.9)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Mediocre</td>
+
                                                        <td>19</td>
+
                                                        <td>3(18.5)</td>
+
                                                        <td>6(31.6)</td>
+
                                                        <td colspan="3">10(52.6)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Work hard</td>
+
                                                        <td>11</td>
+
                                                        <td>1(9.1)</td>
+
                                                        <td>5(45.5)</td>
+
                                                        <td colspan="3">5(45.5)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td>Number of friends</td>
+
                                                        <td>None</td>
+
                                                        <td>1</td>
+
                                                        <td>0</td>
+
                                                        <td>0</td>
+
                                                        <td>1(100.0)</td>
+
                                                        <td>-2</td>
+
                                                        <td>0</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>1-2</td>
+
                                                        <td>18</td>
+
                                                        <td>3(16.7)</td>
+
                                                        <td>10(55.6)</td>
+
                                                        <td colspan="3">5(27.8)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>3-5</td>
+
                                                        <td>31</td>
+
                                                        <td>4(12.9)</td>
+
                                                        <td>12(38.7)</td>
+
                                                        <td colspan="3">15(48.4)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>6-10</td>
+
                                                        <td>35</td>
+
                                                        <td>12(34.4)</td>
+
                                                        <td>15(42.9)</td>
+
                                                        <td colspan="3">8(22.9)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>&gt;10</td>
+
                                                        <td>46</td>
+
                                                        <td>13(28.3)</td>
+
                                                        <td>23(50.0)</td>
+
                                                        <td colspan="3">10(21.7)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td>Status of parents' working out of town</td>
+
                                                        <td>Both</td>
+
                                                        <td>43</td>
+
                                                        <td>11(25.6)</td>
+
                                                        <td>17(39.5)</td>
+
                                                        <td>15(34.9)</td>
+
                                                        <td>-1</td>
+
                                                        <td>0</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Father</td>
+
                                                        <td>48</td>
+
                                                        <td>8(16.7)</td>
+
                                                        <td>26(54.2)</td>
+
                                                        <td colspan="3">14(29.2)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Mother</td>
+
                                                        <td>11</td>
+
                                                        <td>3(27.3)</td>
+
                                                        <td>4(36.4)</td>
+
                                                        <td colspan="3">4(36.4)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Neither</td>
+
                                                        <td>29</td>
+
                                                        <td>10(34.5)</td>
+
                                                        <td>13(44.8)</td>
+
                                                        <td colspan="3">6(20.7)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td>Relationship with parents</td>
+
                                                        <td>Unhappy</td>
+
                                                        <td>9</td>
+
                                                        <td>1(11.1)</td>
+
                                                        <td>6(66.7)</td>
+
                                                        <td>2(22.2)</td>
+
                                                        <td>-2</td>
+
                                                        <td>0</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Not bad</td>
+
                                                        <td>15</td>
+
                                                        <td>1(6.7)</td>
+
                                                        <td>9(60.0)</td>
+
                                                        <td colspan="3">5(33.3)</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td></td>
+
                                                        <td>Lovely</td>
+
                                                        <td>57</td>
+
                                                        <td>18(31.6)</td>
+
                                                        <td>20(35.1)</td>
+
                                                        <td colspan="3">19(33.3)</td>
+
                                                    </tr>
+
                                                </tbody>
+
                                            </table>
+
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
Line 815: Line 929:
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <h3>Bandhnu course</h3>
+
                        <h2>3.2 Environmental Protection Agency / Company</h2>
                         <div class="image"><img alt="Bandhnu course"
+
                        <p>In daily life, textile printing and dyeing industry is one of the industries with the
                                 src="https://static.igem.org/mediawiki/2021/8/8a/T--HUST-China--img--humanpractice--HP-4.2.16.png"
+
                            greatest degree of environmental pollution. The existing dyeing technology is mainly based
                                 style="width: 90%" />
+
                            on chemical dyes, which will bring pollution of air, water and solid wastes. Pollutants such
                             <p>Figure 18: Bandhnu course</p>
+
                            as NPE and aromatic carcinogens can cause irreversible damage to human body and surrounding
 +
                            environment. In the long run there will be huge economic losses. In recent years, chemical
 +
                            dyes have gradually retreated, and plant hair dyes have emerged. Using plant dyes to dye
 +
                            hair will not only minimize the harm to human body, but also zero pollution to the
 +
                            environment due to the source of biological materials. However, because of various problems
 +
                            of natural dyes, it is difficult to open the market. Mr. Tony adopts microbial fermentation,
 +
                            which not only needs to avoid various problems in the production of plant dyes, but also
 +
                            provides new methods for the production of traditional plant dyes. In addition, in the
 +
                            fermentation process, the precursors and intermediate products of dyes we adopt should be
 +
                            environmentally friendly with few by-products and meet the needs of environmental
 +
                            protection. Based on biological science, we need to produces products eco-friendly and meet
 +
                            the needs of the public. At the same time, in order to avoid the overflow of engineering
 +
                            bacteria in the environment, we need to adopt strict sterilization in the production
 +
                            process, optimize the fermentation process, not only reduce the probability of contamination
 +
                            of miscellaneous bacteria in the fermentation process, but also eliminate the possibility of
 +
                            biological contamination caused by the overflow of engineering bacteria into the
 +
                            environment.</p>
 +
                         <h3>Jiangsu Ronghui Environmental Protection Equipment Co., Ltd.</h3>
 +
                         <div class="image"><img alt="Jiangsu Ronghui Environmental Protection Equipment Co., Ltd."
 +
                                 src="https://static.igem.org/mediawiki/2021/b/b5/T--HUST-China--img--humanpractice--HP-1.3.1.3.png"
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                                 style="width: 50%" />
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                             <p>Figure 15: Jiangsu Ronghui Environmental Protection Equipment Co., Ltd.</p>
 
                         </div>
 
                         </div>
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Bandhnu-course"
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                        <p>A very good dye product that avoids pollution from the production process achieve the
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                            integration of enterprise production and biotechnology to solve environmental problems from
 +
                            the root causes is needed.</p>
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                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Ronghui"
 
                                 data-toggle="modal" type="button">Read More</button>
 
                                 data-toggle="modal" type="button">Read More</button>
                             <div aria-hidden="true" aria-labelledby="Bandhnu-courseLabel" class="modal fade"
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                             <div aria-hidden="true" aria-labelledby="RonghuiLabel" class="modal fade"
                                 data-backdrop="static" data-keyboard="false" id="Bandhnu-course" tabindex="-1">
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                                 data-backdrop="static" data-keyboard="false" id="Ronghui" tabindex="-1">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                     <div class="modal-content">
 
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                                         <div class="modal-header">
                                             <h5 class="modal-title" id="Bandhnu-courseLabel">Bandhnu-course</h5>
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                                             <h5 class="modal-title" id="RonghuiLabel">Ronghui</h5>
 
                                         </div>
 
                                         </div>
 
                                         <div class="modal-body">
 
                                         <div class="modal-body">
                                             <p>On the basis of project Mr. Tony, we established a bandhnu course of
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                                             <p>Gaocheng is the hometown of China's environmental protection and the base
                                                 interest every Monday, Wednesday and Friday and a specialized one in
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                                                of China's environmental protection industry, among which environmental
                                                 Class one, Grade six. We bought pigment, enhancer, white T-shirts, hats
+
                                                 protection companies are dotted dealing with various kinds of pollution,
                                                 and scarves. We explained the history of bandhnu and modern techniques,
+
                                                 including waste water, waste gas, waste residue and so on. This time, we
                                                 which could increase students' manipulative ability and creativity
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                                                interviewed engineer Wang Yong, who has been in the environmental
                                                 during application. In the farewell party at the end of the
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                                                 protection industry for 10 years. He has accumulated a wealth of
                                                 education-aiding course, T-show was held to encourage students daring to
+
                                                experience in this industry, starting with electric welding materials
                                                 show off their product and personality. Through the charm of colour,
+
                                                 according to drawings, contracting orders by himself, designing sewage
                                                 students enhanced their relationship with each other and obviously,
+
                                                 treatment equipment from scratch and realizing it. In the course of the
                                                 removed some of the lonliness.</p>
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                                                interview, we learned about the principle and effect of the sewage
                                            <table>
+
                                                 treatment equipment, visited the equipment manufacturing workshop, and
                                                 <thead>
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                                                 understood the internal structure of all kinds of equipment. We also
                                                    <tr>
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                                                 deeply realized steps that sewage needs to go through from seriously
                                                        <th>photo</th>
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                                                 exceeding the standard of pollutants to being able to discharge safely.
                                                        <th>description</th>
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                                                 Through this interview, we have a deeper understanding of dye pollution
                                                    </tr>
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                                                 treatment. And the relevant indicators for dealing with dye pollution in
                                                 </thead>
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                                                 today's environmental protection industry. Mr.Tony needs to adhere to
                                                 <tbody>
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                                                the principle that it is harmless to the human body and good to the
                                                    <tr>
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                                                environment in the production process. From the input of raw materials
                                                        <td><img src="https://static.igem.org/mediawiki/2021/0/04/T--HUST-China--img--humanpractice--HP-4.2.17.png"
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                                                to the acquisition of the final product to the use of the product, the
                                                                width="40%" /></td>
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                                                intermediate products need to be harmless to the environment and there
                                                        <td>Dyeing in the bandhnu course</td>
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                                                are few by-products as far as possible, so as to meet the needs of
                                                    </tr>
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                                                environmental protection. After communicating with engineer Wang Yong,
                                                    <tr>
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                                                he said that the pollution factors in the pollutants emitted by dye
                                                        <td><img src="https://static.igem.org/mediawiki/2021/d/d7/T--HUST-China--img--humanpractice--HP-4.2.18.png"
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                                                enterprises are complex and difficult to deal with. Therefore, high
                                                                width="40%" /></td>
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                                                pollution enterprises like these can not completely rely on follow-up
                                                        <td>Displaying bandhnu product from one of the students</td>
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                                                sewage treatment. In order to solve the problem of high pollution, we
                                                    </tr>
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                                                must start from the source. Therefore, we need a very good dye product
                                                    <tr>
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                                                that avoids pollution from the production process. It is also to cater
                                                        <td><img src="https://static.igem.org/mediawiki/2021/e/ef/T--HUST-China--img--humanpractice--HP-4.2.19.png"
+
                                                to the trend of pollution control around the world in the future, and
                                                                width="40%" /></td>
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                                                really achieve the integration of enterprise production and
                                                        <td>Laughter in their own bandhnu clothes on the T show</td>
+
                                                 biotechnology to solve environmental problems from the root causes.</p>
                                                    </tr>
+
                                                    <tr>
+
                                                        <td><img src="https://static.igem.org/mediawiki/2021/8/83/T--HUST-China--img--humanpractice--HP-4.2.20.png"
+
                                                                width="40%" /></td>
+
                                                        <td>Group photo of students with their own bandhnu product and
+
                                                            joy</td>
+
                                                    </tr>
+
                                                    <tr>
+
                                                        <td><img src="https://static.igem.org/mediawiki/2021/a/ab/T--HUST-China--img--humanpractice--HP-4.2.21.png"
+
                                                                width="40%" /></td>
+
                                                        <td>'Models' awaiting to going on the stage</td>
+
                                                    </tr>
+
                                                 </tbody>
+
                                            </table>
+
 
                                         </div>
 
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Line 884: Line 1,008:
 
                             </div>
 
                             </div>
 
                         </div>
 
                         </div>
                         <h2>Bioethics week debate</h2>
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                         <h3>Environmental Protection Bureau of Yantai</h3>
                         <div class="image"><img alt="Bioethics week debate"
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                         <div class="image"><img alt="Environmental Protection Bureau of Yantai"
                                 src="https://static.igem.org/mediawiki/2021/7/75/T--HUST-China--img--humanpractice--HP-4.2.22.png"
+
                                 src="https://static.igem.org/mediawiki/2021/a/a6/T--HUST-China--img--humanpractice--HP-1.3.1.4.png"
                                 style="width: 90%" />
+
                                 style="width: 50%" />
                             <p>Figure 19: Bioethics week debate</p>
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                             <p>Figure 16: Environmental Protection Bureau of Yantai</p>
 
                         </div>
 
                         </div>
                         <p>With the invitation of the HZAU-China team, HUST-China travelled to Huazhong Agricultural
+
                         <p>Try to make good products that can protect the ecology, protect the environment and meet the
                             University to participate in the Bioethics Week. Both sides launched a friendship debate ---
+
                            needs of the public, so as to achieve a win-win situation of economic development and
                            ' artificial intelligence / humans are more likely to lead to human destruction '. After the
+
                             environmental protection.</p>
                             fierce debate between the two sides, the questioning of the audience, the comments of the
+
                        <div class="modal-btn"><button class="btn btn-warning pull-right"
                            judges and the sharing of points of views, the audience had a new understanding of life
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                                data-target="#Environmental-Protection-Bureau-of-Yantai" data-toggle="modal"
                            ethics. We do not want human civilization to really go to the day of destruction, but we
+
                                type="button">Read More</button>
                            continue to imagine how it may be destroyed. So we should eliminate and avoid errors, and
+
                             <div aria-hidden="true" aria-labelledby="Environmental-Protection-Bureau-of-YantaiLabel"
                            then move forward more cautiously. It is beyond dispute that through this event, we can be
+
                                class="modal fade" data-backdrop="static" data-keyboard="false"
                            more cautious and also more courageous in the face of scientific progress and assume the
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                                id="Environmental-Protection-Bureau-of-Yantai" tabindex="-1">
                            responsibility of life science practitioners.</p>
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                                <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
                        <h2>Beijing voluntary lecture</h2>
+
                                    <div class="modal-content">
                        <div class="image"><img alt="Beijing voluntary lecture"
+
                                        <div class="modal-header">
                                src="https://static.igem.org/mediawiki/2021/d/d6/T--HUST-China--img--humanpractice--HP-4.2.23.png"
+
                                            <h5 class="modal-title" id="Environmental-Protection-Bureau-of-YantaiLabel">
                                style="width: 90%" />
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                                                Environmental-Protection-Bureau-of-Yantai</h5>
                            <p>Figure 20: Beijing voluntary lecture</p>
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                                        </div>
 +
                                        <div class="modal-body">
 +
                                            <p>In the process of interviewing Director Zhang of the Environmental
 +
                                                Protection Bureau, we first learned about the current tasks and
 +
                                                management strategies of the Environmental Protection Bureau, and
 +
                                                learned that the biggest difficulty in the current supervision of the
 +
                                                Environmental Protection Bureau lies in the irregular emission of waste
 +
                                                gas and dust from printing and dyeing factories and foundry plants,
 +
                                                which precisely reflects the current pollution problem of traditional
 +
                                                chemical dyes, and it will be one of the problems that our iGEM team is
 +
                                                committed to solve. After that, we have a detailed understanding of the
 +
                                                treatment and regulatory policies and relevant standards of the
 +
                                                discharge of chemical dyes, which further confirms the conclusion that
 +
                                                the pollution problem of chemical dyes is serious in the previous
 +
                                                research work. In addition, Director Zhang also revealed that at
 +
                                                present, high-tech zones have relevant support policies for
 +
                                                environmental protection and new technology and new energy industries,
 +
                                                while for enterprises with high pollution and high energy consumption,
 +
                                                the Environmental Protection Bureau is scheduled to move them out step
 +
                                                by step. Through investigation, we have more clearly identified the
 +
                                                serious problem of high pollution in traditional chemical dye factories
 +
                                                and realized the potential advantages of Mr.Tony in producing natural
 +
                                                dyes. Director Zhang also suggested that Mr.Tony not only needs to solve
 +
                                                the problem of environmental pollution caused by enterprise emissions
 +
                                                from the source, but also the raw materials and strains used in the
 +
                                                fermentation process need to be environmentally friendly, based on
 +
                                                bioscience, to make good products that can protect the ecology, protect
 +
                                                the environment and meet the needs of the public, so as to achieve a
 +
                                                win-win situation of economic development and environmental protection.
 +
                                            </p>
 +
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 +
                                </div>
 +
                            </div>
 
                         </div>
 
                         </div>
                         <p>In order to pay attention to the popularization and rapid development of synthetic biology,
+
                        <h2>3.3 Chinese traditional Plant Dye Workshop</h2>
                             HUST-China team responded to the call of CAU-China to join in voluntary lectures on
+
                         <p>Tie-dyeing set off a fashion craze because of its gorgeous and simple design. However, due to
                             synthetic biology for all college students in Beijing community. This voluntary lecture,
+
                            the promulgation of the national policy on environmental protection, the tie-dyeing industry
                             faced with college students in Beijing, talked a lot about the history of synthetic biology,
+
                             is becoming extinct, and because of the serious aging of the employees, the tie-dyeing
                             basic knowledge of life science, brief introduction and prospect of synthetic biology, and
+
                             technology has the risk of losing generations. At the same time, the key point of tie-dyeing
                             introduction of iGEM events, which not only expanded the influence of iGEM events, but also
+
                             technology is to use plants for dyeing, but in the production process of tie-dyeing, plant
                             comprehensively popularized synthetic biology. In addition, each team carried on the project
+
                             dyes have low productivity, raw materials are more expensive and rare, which makes more
                             display in turn, really let the students combine with the actual application to consolidate
+
                            convenient and fast chemical dyeing to replace plant dyeing in a large area. Mr.Tony was
                             the basic knowledge of synthetic biology. We also arranged interesting knowledge answers and
+
                             born in the laboratory. In order to synthesize natural dyes by engineering yeast, it is
                             questions part, which narrowed the gap between students, and comprehensively answered the
+
                            necessary to solve the problems of complex preparation process and low purity of dyes in the
                             questions of students.</p>
+
                             traditional tie-dyeing process, so as to realize the mass production of natural dyes. In the
                         <div class="image"><img alt="Beijing voluntary lecture"
+
                             follow-up, the team needs to explore the optimal dyeing conditions and color mixing
                                 src="https://static.igem.org/mediawiki/2021/b/b4/T--HUST-China--img--humanpractice--HP-4.2.24.png"
+
                             experiments of the dyes, so as to provide the possibility for showing a variety of colors in
                                 style="width: 90%" />
+
                            the tie-dyeing process. At the same time, it is also very important to realize the
                             <p>Figure 21: Beijing voluntary lecture</p>
+
                             quantitative analysis of plant dyes and clarify the chemical composition, optimal dosage and
 +
                             biochemical principles of dyes and auxiliaries in the dyeing process through experiments. so
 +
                            as to help tie-dyeing, a traditional handicraft industry on a large scale. Let the
 +
                            tie-dyeing process which strongly depends on traditional dyes go further and grow stronger.
 +
                        </p>
 +
                        <h3>Lan dyeing workshop</h3>
 +
                         <div class="image"><img alt="Lan dyeing workshop"
 +
                                 src="https://static.igem.org/mediawiki/2021/d/d2/T--HUST-China--img--humanpractice--HP-1.3.1.7.png"
 +
                                 style="width: 50%" />
 +
                             <p>Figure 17: Lan dyeing workshop</p>
 
                         </div>
 
                         </div>
                         <p>After the lecture, many listeners said that this event made them have some preliminary
+
                         <p>The development of any dye needs to clarify the chemical composition and optimal dosage of
                            understanding of the principles and applications of synthetic biology. The projects
+
                             dyes and auxiliaries in the dyeing process, as well as the biochemical principles in the
                             introduced by the lecturers were very meaningful and promising, and they said that they
+
                             dyeing process, so as to help more craftsmen accurately grasp the amount of dyes.</p>
                            would continue to further understand and learn relevant knowledge in the future.</p>
+
                         <div class="modal-btn"><button class="btn btn-warning pull-right"
                        <h1>4 Commercial products</h1>
+
                                data-target="#Lan-dyeing-workshop" data-toggle="modal" type="button">Read More</button>
                        <h2>Software:</h2>
+
                             <div aria-hidden="true" aria-labelledby="Lan-dyeing-workshopLabel" class="modal fade"
                        <ol>
+
                                 data-backdrop="static" data-keyboard="false" id="Lan-dyeing-workshop" tabindex="-1">
                            <li>
+
                                <p>Based on the algorithm framework of YOLOv5, we customize a set of data sets and data
+
                                    labels on the micronucleus count of vicia faba root tips, which includes hundreds of
+
                                    typical chromatin and micronucleus images. Based on this, a model is trained to
+
                                    count the micronucleus of vicia faba root tips under the experimental conditions of
+
                                    this project, and then determine the toxicity of various dyes. We opened the
+
                                    download of this model on GITHUB, and introduced the self-defined process in MODEL (
+
                                    if it is only used for the identification of micronucleus ) to ensure that each user
+
                                    can train a counting model suitable for its personal experimental conditions.</p>
+
                            </li>
+
                            <li>
+
                                <p>According to the results of hair dyeing experiments, we developed a set of color
+
                                    prediction algorithms, and developed a small program named Dye Your Hair-Mr. Tony
+
                                    HUST on WeChat. This widget returns the expected hair color results based on the
+
                                    type of pigment you submit and the dyeing time. The selection of single / two
+
                                    pigments is currently supported. Maintenance will continue to be updated.</p>
+
                            </li>
+
                        </ol>
+
                        <p>For detailed information , please refer to software part</p>
+
                        <h2>Hardware:</h2>
+
                        <ol>
+
                            <li>
+
                                <p>We have designed a portable and easy-to-use fermenter whose fermentation chamber and
+
                                    reaction chamber are integrated into one device. After fermentation, the working
+
                                    enzyme can pass through the filter and produce pigments in the reaction chamber only
+
                                    by inverting the bottle, which will make it more convenient for the production and
+
                                    storage of pigments and provide convenience for users.</p>
+
                             </li>
+
                            <li>
+
                                <p>We also designed a dye comb to cooperate with the use of dye in the fermentor. This
+
                                    tool will enable users to customize the entire process from pigment production to
+
                                    hair dyeing.</p>
+
                            </li>
+
                        </ol>
+
                        <p>For detailed information , please refer to hardware part</p>
+
                        <h2>Business plan:</h2>
+
                        <p>In order to make a further progress of the following projects and exaggerate the influence of
+
                            Mr. Tony brand, we have also formed a business plan to continue to promote a healthy,
+
                            humanized and reversible biological perm and dye system.</p>
+
                         <div class="modal-btn"><button class="btn btn-warning pull-right" data-target="#Business-plan"
+
                                data-toggle="modal" type="button">Read More</button>
+
                             <div aria-hidden="true" aria-labelledby="Business-planLabel" class="modal fade"
+
                                 data-backdrop="static" data-keyboard="false" id="Business-plan" tabindex="-1">
+
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                 <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 
                                     <div class="modal-content">
 
                                     <div class="modal-content">
 
                                         <div class="modal-header">
 
                                         <div class="modal-header">
                                             <h5 class="modal-title" id="Business-planLabel">Business-plan</h5>
+
                                             <h5 class="modal-title" id="Lan-dyeing-workshopLabel">Lan-dyeing-workshop
 +
                                            </h5>
 +
                                        </div>
 +
                                        <div class="modal-body">
 +
                                            <p>The plant dyeing process is quite complex and is gradually replaced by
 +
                                                chemical fabrics, so the number of plant dyeing craftsmen continues to
 +
                                                decrease. We interviewed teacher Peng Shuangying, who was born into a
 +
                                                family of blue-dye craftsmen. She studied Blue-dye from an early age,
 +
                                                but she did not continue to work in the industry because of the narrow
 +
                                                market. Later, she innovated the type, color, pattern and technology of
 +
                                                weaving and dyeing, created her own brand, and was awarded the title of
 +
                                                "inheritor of vegetation dyeing of Pujiang County Intangible Cultural
 +
                                                Heritage".</p>
 +
                                            <p>Peng explained to us the complete dyeing process from plant Radix
 +
                                                Isatidis to indigo dye and showed the mural of blue dyeing process. In
 +
                                                her studio, there are all kinds of blue-dyed products, including toys,
 +
                                                canvas bags, bedding, screens, fans, cloth chairs and clothing. She also
 +
                                                briefly introduced various dyeing processes and natural dyes of other
 +
                                                colors and made use of batik, tie dyeing, knock dyeing and other
 +
                                                techniques, together with a variety of patterns, to turn blue-dyed
 +
                                                fabrics into works of art and coruscate new vitality. However, at
 +
                                                present, traditional craftsmanship is often empirical, so there is an
 +
                                                urgent need to combine traditional craftsmanship with science and
 +
                                                technology to achieve quantitative analysis and clarify the chemical
 +
                                                composition and biochemical principles of additives in plant dyeing
 +
                                                processes to make traditional dyes go further. The research and
 +
                                                development of any dye needs to clarify the chemical composition and
 +
                                                optimal dosage of dyes and auxiliaries in the dyeing process, as well as
 +
                                                the biochemical principles in the dyeing process, so as to help more
 +
                                                craftsmen accurately grasp the amount of dyes but not only rely on oral
 +
                                                experience, thus making the scale of traditional handicrafts possible.
 +
                                                In order to make the traditional dyeing process go further and grow
 +
                                                stronger.</p>
 +
                                        </div>
 +
                                        <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 +
                                                type="button">Close</button></div>
 +
                                    </div>
 +
                                </div>
 +
                            </div>
 +
                        </div>
 +
                        <h3>Dali Puzhen Museum</h3>
 +
                        <div class="image"><img alt="Dali Puzhen Museum"
 +
                                src="https://static.igem.org/mediawiki/2021/5/54/T--HUST-China--img--humanpractice--HP-1.3.1.8.png"
 +
                                style="width: 50%" />
 +
                            <p>Figure 18: Dali Puzhen Museum</p>
 +
                        </div>
 +
                        <p>Mr.Tony is needed to solve the problems of complex dye preparation process and low purity of
 +
                            dyes used in traditional tie-dyeing process, so as to achieve mass production of natural
 +
                            dyes, to help more craftsmen to achieve large-scale, better promotion of traditional
 +
                            technology.</p>
 +
                        <div class="modal-btn"><button class="btn btn-warning pull-right"
 +
                                data-target="#Dali-Puzhen-Museum" data-toggle="modal" type="button">Read More</button>
 +
                            <div aria-hidden="true" aria-labelledby="Dali-Puzhen-MuseumLabel" class="modal fade"
 +
                                data-backdrop="static" data-keyboard="false" id="Dali-Puzhen-Museum" tabindex="-1">
 +
                                <div class="modal-dialog modal-dialog-scrollable modal-dialog-centered modal-xl">
 +
                                    <div class="modal-content">
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                                        <div class="modal-header">
 +
                                            <h5 class="modal-title" id="Dali-Puzhen-MuseumLabel">Dali-Puzhen-Museum</h5>
 +
                                        </div>
 +
                                        <div class="modal-body">
 +
                                            <p>At the Puzhen tie-dyeing Museum, there are a number of dyed fabrics
 +
                                                waiting to be oxidized and dried on the outside, most of them have a
 +
                                                large area and exquisite designs, and then we can see that several large
 +
                                                dye vats are filled with dyes, emitting a special aroma. There are many
 +
                                                mounted handicrafts in the dyeing workshop, with a variety of
 +
                                                craftsmanship, styles and categories, with ulterior motives in the
 +
                                                placement arrangement. At the same time, even the ceiling of the
 +
                                                tie-dyeing workshop is covered with dye cloth. Being in the dyeing
 +
                                                workshop and looking around, the line of sight is full of blue and white
 +
                                                patterns, making people feel the strong atmosphere of tradition and
 +
                                                folklore. However, the incompatibility between natural
 +
                                                dyeing&amp;traditional processes and large-scale production also
 +
                                                appears, especailly in small workshops, while the productivity of plant
 +
                                                dyes is low, and raw materials are more expensive and rarer, so plant
 +
                                                dyeing shows great deficiencies compared with more convenient and rapid
 +
                                                chemical dyeing, so Mr.Tony is needed to solve the problems of complex
 +
                                                dye preparation process and low purity of dyes used in traditional
 +
                                                tie-dyeing process, so as to achieve mass production of natural dyes, to
 +
                                                help more craftsmen to achieve large-scale, better promotion of
 +
                                                traditional technology.</p>
 +
                                        </div>
 +
                                        <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 +
                                                type="button">Close</button></div>
 +
                                    </div>
 +
                                </div>
 +
                            </div>
 +
                        </div>
 +
                        <h3>Lanxu ancient tie-dye experience Hall</h3>
 +
                        <p>It is necessary to realize the quantitative analysis of plant dyes, clarify the chemical
 +
                            composition of dyes and dyeing aids in the dyeing process, the optimal dosage and the
 +
                            biochemical principles in the dyeing process, so as to provide solutions to the traditional
 +
                            processes facing the crisis of extinction.</p>
 +
                        <div class="image"><img alt="Lanxu ancient tie-dye experience Hall"
 +
                                src="https://static.igem.org/mediawiki/2021/3/30/T--HUST-China--img--humanpractice--HP-1.3.1.9.png"
 +
                                style="width: 50%" />
 +
                            <p>Figure 19: Lanxu ancient tie-dye experience Hall</p>
 +
                        </div>
 +
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                                                Lanxu-ancient-tie-dye-experience-Hall</h5>
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                                        </div>
 +
                                        <div class="modal-body">
 +
                                            <p>In the Dali Lanxu tie-dyeing experience Museum, we learned that the
 +
                                                tie-dyeing process is divided into two processes: tying and dyeing. The
 +
                                                technique of tying determines the trend and basic shape of the pattern.
 +
                                                Complex and exquisite patterns often need to be embroidered with basic
 +
                                                pattern units in advance, which is similar to the principle of cutting
 +
                                                window grilles, showing the final pattern symmetrically, and then in
 +
                                                interviews with staff and follow-up visits, we deeply understand that
 +
                                                traditional and natural as the keyword of this old craft, have been paid
 +
                                                more and more attention by the society in recent years, and also meet
 +
                                                the consumption needs of people in the new era. However, the failure of
 +
                                                natural dyeing and traditional technology to achieve large-scale
 +
                                                production is still a huge problem faced by the traditional process, and
 +
                                                it is precisely because of this that the main production mode of the
 +
                                                traditional handicraft industry is still small-scale operation, and
 +
                                                heavily relies on tourism and other industries. In addition to producing
 +
                                                dyes, our team also needs to explore the optimal dyeing conditions and
 +
                                                color mixing experiments in order to provide the possibility of showing
 +
                                                a variety of colors in the tie-dyeing process and mass production. At
 +
                                                the same time, it is necessary to realize the quantitative analysis of
 +
                                                plant dyes, clarify the chemical composition of dyes and dyeing aids in
 +
                                                the dyeing process, the optimal dosage and the biochemical principles in
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                                                the dyeing process, so as to provide solutions to the traditional
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                                                processes facing the crisis of extinction.</p>
 
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                                        <div class="modal-body"><iframe height="500px"
 
                                                src="https://static.igem.org/mediawiki/2021/7/7a/T--HUST-China--doc--Mr_Tony_commercial_plan.pdf" width="100%"></iframe></div>
 
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
                                         <div class="modal-footer"><button class="btn btn-danger" data-dismiss="modal"
 
                                                 type="button">Close</button></div>
 
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                         </div>
                        <h1>5 Open source project sharing</h1>
 
                        <ol>
 
                            <li>
 
                                <p>MCN counter : Based on the algorithm framework of YOLOv5, we customize a set of data
 
                                    sets and data labels on the micronucleus count of vicia faba root tips, which
 
                                    includes hundreds of typical chromatin and micronucleus images. Based on this, a
 
                                    model is trained to count the micronucleus of vicia faba root tips under the
 
                                    experimental conditions of this project, and then determine the toxicity of various
 
                                    dyes. This greatly liberates the manpower and the heavy reading work will be done by
 
                                    the computer. We opened the download of this model on GITHUB, and introduced the
 
                                    self-defined process in MODEL ( if it is only used for the identification of
 
                                    micronucleus ) to ensure that each user can train a counting model suitable for its
 
                                    personal experimental conditions.</p>
 
                            </li>
 
                            <li>
 
                                <p>Dyeing predictor : We built several BP neural networks in python3 + tensorflow +
 
                                    keras environment to predict the dyeing results according to the type, concentration
 
                                    and time of pigment. If you ' ve read the relevant parts of MODEL in detail, you '
 
                                    ll realize that this network is actually for all fiber dyeing - pigment attachment
 
                                    systems. Therefore, you can completely customize your own prediction model according
 
                                    to this network structure. This model contributes more in the mathematical part of
 
                                    determining data labels and activation functions of each layer. You can learn more
 
                                    in the relevant parts of MODEL.</p>
 
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                                     <p>Marvellous! Human practice conducted by your team not only served as a guide for
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                                     <p>Unbelievable! You have done such a thorough and detailed job just in the step of
                                        the project, but also contributed to the world by disseminating synthetic
+
                                         identifying the problem!! Let's get to the second part: '<a
                                         biology knowledge and conveying positive energy!! Now, let's have a look at <a
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                                             href="https://2021.igem.org/Team:HUST-China/Human_Practices_02">Formulate
                                             href="https://2021.igem.org/Team:HUST-China/Model">Modeling
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                                            implementation plan</a>' right away!</p>
                                        </a>part!</p>
+
 
                                 </div>
 
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Team:HUST-China/Human Practices 01

Human Practices | iGEM HUST-China

Human Practices

Part 01 Discover and analyze the problems


At present, perming and dyeing hair has become a trend and a new trend of beauty. However, in China, the popularity of perming and dyeing is not as expected. Many people have the desire to improve their image by perming and dyeing, but it has not been realized in the end. After consulting the information, we found that there may be the following reasons: first of all, the most common chemical hair dyes on the market will cause considerable harm to the human body; secondly, the domestic public may have a certain degree of prejudice against the exaggerated styling; in addition, the potential value of the plant hair dye market is great, but the market share is very low.

In order to solve the problems above, our team decided to develop a healthy, humanized and reversible integrated ironing and dyeing product. While solving the problems, the plant dyes we choose have also achieved an environmentally friendly breakthrough and contributed to the world's environmental protection.

In order to confirm our cause analysis and extensively investigate the opinions of the public, we have conducted extensive surveys on people of different identities, consumers, entrepreneurs and relevant stakeholders by means of interviews, questionnaires and so on.

1 Interview

In order to explore consumers' concerns about the health hazards of chemical dyes and people's attitudes towards perming and dyeing, we selected a number of people of different ages and occupations to conduct interviews

1.1 Health Concerns

Young people

Young people

Figure 1: Young people

Chemical dyes do great harm to our hair, but nowadays many college students know the harm, but still choose to dye their hair. Today, college students seem to have become the main consumer group in the hair dyeing market. For this reason, we interviewed several college students. Through our interviews and investigations, we found that most college students are very aware of the harm caused by hair dyeing, and they are also very worried about it. After the interview, we found that some people who use chemical dyes to dye their hair more or less have hair health problems, such as poorer hair quality, hair bifurcations and so on. Frequent use of chemical dyes leads to the deterioration of hair quality. The sequelae of appearance reduction such as hair loss, hair loss and bifurcation are the main reasons for college students to give up dyeing their hair. Most students who have dyed their hair or want to dye their hair say they will choose not to dye their hair if the dye makes their hair worse. For natural plant hair dyeing products, most students are very willing to try and accept, but a small number of students say that they doubt whether natural pigment hair dyeing can have the advantages of various color selectivity and high color fastness like chemical reagents. Some students said that the price of natural plant hair dyeing product is also the prime consideration of their choice of dye. College students who do not have a fixed income may have no choice but to choose relatively cheap chemical dyes.

Elderly people

In order to investigate the views of different age groups on hair dyeing and hair care, we interviewed the middle-aged and elderly people by means of online video. Through our interview, we found that more and more middle-aged and elderly people choose to dye their hair because of the increase in gray hair. Most people say that they are very supportive of hair dyeing, which can cover their silver hair and make a person more energetic in appearance, and their mentality will become younger when they see themselves younger. Many people say this is an act of killing two birds with one stone. Through our survey, we also found that people today are under great social pressure and a fast pace of life, resulting in a continuous decline in the age of silver-haired people, and many middle-aged people in their forties and fifties (not really old age) also have a lot of gray hair. This group is also one of the main consumers in the hair dyeing market. Compared with college students, this kind of hair dye consumers are different in their higher consumption and more pursuit of quality and raw materials. For them, the safety and health of dyes are more important. Many people choose dyes with advertising words such as "traditional Chinese medicine", "natural" and "health". They value health more than a variety of colors and cheap prices. Therefore, we believe that middle-aged and elderly group will be the main consumers of natural dyes.

Elderly people

Figure 2: Elderly people

1.2 Social Attitude

In addition to the health effects of dye reagents, the public's attitude towards hair dyeing is also an important factor restricting the freedom to perm and dye hair. In China, especially among the older generation, perming and dyeing hair, especially those with exaggerated shapes and colors, are often regarded as informal or too lively. Considering the universal practicality of the product, we investigated the attitudes of different classes towards hair dyeing. The overall survey results show that most people are not resistant to hair dyeing, and many people say they want to try. The main problem is that if you want to dye your hair, you need to dye it back, which not only do harms the quality of your hair, but also makes it more troublesome. Therefore, we imagine that the "fade" function will be added to the project, and the color can be faded by using other substances after dyeing the hair.

Company and employees

Company-and-employees

Figure 3: Company-and-employees

For individuals, dyeing hair shows personality, represents avantgarde and their attention towards appearance. But for employees at work, they may be tied up in order to maintain their appearance at a critical moment. For companies, employees should be loyal to their work when they are at work. If there are more such employees, it will increase unnecessary economic and time costs for the company. The boss said that enterprises need to have enterprise culture, regulations, and some restrictions. Company needs to regulate employees' behavior and appearance during work in order to maintain the image of the company. At the same time, the boss also said that apart from working hours, the company has no objection but respect all ideas of employees to manage their appearance and publicize their personality. When interviewing employees, we come to the conclusion that although most people have the desire to dye their hair, it is inconvenient or troublesome for them to go to work. Although the company does not restrict the behavior of individuals during their non-working hours, dyeing their hair after work and back to work for a day or two is both time-consuming, and it will do more harm than good for people who work in society. The person in charge said that he very supports the idea that employees have such an idea to dress themselves up. Employees do what they want to do without violating the company rules, and keeping a happy mood is also a guarantee for the boss

Teachers and students

Teachers-and-students

Figure 4: Teachers-and-students

We interviewed a student and his counselor teacher from Huazhong University of Science and Technology. The feedback is that the teacher has no objection to hair dyeing. However, he believes that when a student is still a student at school, his main mind should be on study. There is still a lot of opposition to hair dyeing during class. Holidays, winter and summer holidays, such holiday time, students can do whatever he likes, including hair dyeing and perming. Students dye and perm their hair is their own choice, if he can feel happy from it, the external improvement will also increase self-confidence. For the students, we interviewed him separately and learned that his idea was similar to that of the counselor. When he's at school, he focuses on his studies and doesn't care about it at all. In addition, too conspicuous hair color, also reflects the individual personality publicity, may attract more attention, but the student's personality is quiet, he chose to give up hair color to maintain his serenity. May be during the holiday, a whim will want to try some new hair color, change the mental outlook. As a result, we are more certain that the market acceptance level of the "fading" design will be very high.

Family.

We came to the houses of several students and had an in-depth understanding of what parents think of hair dyeing. A small number of parents said that they could not accept their children to dye their hair because they would think that their children were not paying attention to their studies. But most parents show respect for their children's choice to dye their hair. They say that times are changing, external changes will not affect internal changes, hair dyeing is only a way to pursue fashion personality, they do not want their children to leave regrets on the way to grow up. After investigation, we found that most parents have something in common, that is, children are required not to dye their hair during school. Other parents say that hair dyeing is more harmful to their hair and do not want their children to try. However, after learning about our faded product concept, they expressed their interest. Such healthy and convenient products can not only allay the worries of parents, but also satisfy the will of children to show personality and pursue themselves.

2 Questionnaire

For thousands of years, people persist in aspiring after beauty, while beauty can't exist without people. Hair perming and dyeing had been a trend of the world since 4000 B.C.. Nowadays, though we could see a blooming hairdressing market of over $380 billion, glance into the deep-seated requirement of the market is needed. To have a clearer sight of this, we initiate a public survey about perming and dyeing and our idea of them.

Sample Status

Male : Female = 4 : 7.

Most around 18-30 and above 45.

50% have job under serious circumstances.

From left to right: Gender, Age, Vocation

Figure 5: From left to right: Gender, Age, Vocation

Did You Dare?!

Most did have perming and dyeing but mostly through the chemical method, and we all know what will happen when it comes to 'chemical' in human body.

From left to right: Have you ever had a perm?, Have you dyed your hair before?

Figure 6: From left to right: Have you ever had a perm?, Have you dyed your hair before?

From left to right: How did you have your perm? What kind of dye did you use?

Figure 7: From left to right: How did you have your perm? What kind of dye did you use?

The Latent Beast

Most people do concern about the danger behind chemical hairdressing. There is scream of new perming and dyeing method with less 'cost'.

From left to right: People's know-about of negative effects of perm, People's know-about of negative effects of dyeing

Figure 8: From left to right: People's know-about of negative effects of perm, People's know-about of negative effects of dyeing

Instant: In and Out, On and Off

Aside from damage, we find out that it is the time cost of getting the hair back to normal that is the main factor blocking people from trying perming and dyeing. Instead of having for days and weeks, what if we could shorten this time window into hours?

From left to right: Why didn't have a perm?, Why didn't have dyeing?

Figure 9: From left to right: Why didn't have a perm?, Why didn't have dyeing?

From left to right: Why don't choose potion perm?, Do you need a product of instant hair customizing or reverse reaction with simple operation.

Figure 10: From left to right: Why don't choose potion perm?, Do you need a product of instant hair customizing or reverse reaction with simple operation.

Mr. Tony Has a Light Touch and a Long Way to Go

Little damage, instant decolorization, DIY, environmentally friendly... With all these shining points, Mr. Tony still has a lot to conquer.

From left to right: Have you ever heard of plant-based dye?, Would you choose plant-based dye?

Figure 11: From left to right: Have you ever heard of plant-based dye?, Would you choose plant-based dye?

From left to right: What do you worry about plant-based dye?, What feature attracts you most?,  Is there anything else you are worry about?

Figure 12: From left to right: What do you worry about plant-based dye?, What feature attracts you most?, Is there anything else you are worry about?

The original questionnaire

3 Key Stakeholder

3.1 Hair Salon

In recent years, consumers' awareness of "green health" is getting stronger and stronger. In barber shops, people are more inclined to healthy and harmless green products than chemical ironing agents that do great damage to their hair. However, in China, the upstream of plant perming and dyeing industry is mainly the extraction of various colored plant components, dye processing equipment, etc. in the process of plant dye production, the problem of long consumption cycle, large input of manpower and material resources and low extraction efficiency cannot be solved well. In the downstream of plant dye industry (like dyeing barbershop), plant dyes are difficult to fix and fast to decolorize. High cost also discourages domestic barbershop from using plant hair dyes. Therefore, with the diversification of market consumption and the need for the pursuit of health, the traditional plant perming and dyeing process needs to absorb modern scientific knowledge and use materialization methods, so as to develop into high-tech fields, improve and enhance its own application value. Mr. Tony needs to grasp this trend, to use microbial fermentation to overcome the problem of low extraction efficiency of components in the traditional industry, and to reduce the production manpower, material resources and time cost, also explore the best coloring conditions of each dye by carrying out relevant experiments. It is also necessary to evaluate the ability of natural dyes to adhere to hair and the effect of natural dyes on hair properties. Pay attention to the decolorization time of dyes while improving color fixation. In the part of hair dyeing, we need to select the perm agent which can easily enter the hair cortex on the basis of the traditional perm principle, so as to meet people's perm requirements healthily without harming the quality of the hair.

3M original barber shop in Jiaozuo , Henan

3M original barber shop in Jiaozuo , Henan

Figure 13: 3M original barber shop in Jiaozuo , Henan

Mr.Tony need to reduce the cost of plant hair dyeing and provide customers with long-lasting and healthy hair dyeing effect.

Plant-based hair dye shop

Plant-based hair dye shop

Figure 14: Plant-based hair dye shop

Mr. Tony need to comply with contemporary consumers in the pursuit of beauty while paying attention to the trend of health and solve the problems of high pollution.

3.2 Environmental Protection Agency / Company

In daily life, textile printing and dyeing industry is one of the industries with the greatest degree of environmental pollution. The existing dyeing technology is mainly based on chemical dyes, which will bring pollution of air, water and solid wastes. Pollutants such as NPE and aromatic carcinogens can cause irreversible damage to human body and surrounding environment. In the long run there will be huge economic losses. In recent years, chemical dyes have gradually retreated, and plant hair dyes have emerged. Using plant dyes to dye hair will not only minimize the harm to human body, but also zero pollution to the environment due to the source of biological materials. However, because of various problems of natural dyes, it is difficult to open the market. Mr. Tony adopts microbial fermentation, which not only needs to avoid various problems in the production of plant dyes, but also provides new methods for the production of traditional plant dyes. In addition, in the fermentation process, the precursors and intermediate products of dyes we adopt should be environmentally friendly with few by-products and meet the needs of environmental protection. Based on biological science, we need to produces products eco-friendly and meet the needs of the public. At the same time, in order to avoid the overflow of engineering bacteria in the environment, we need to adopt strict sterilization in the production process, optimize the fermentation process, not only reduce the probability of contamination of miscellaneous bacteria in the fermentation process, but also eliminate the possibility of biological contamination caused by the overflow of engineering bacteria into the environment.

Jiangsu Ronghui Environmental Protection Equipment Co., Ltd.

Jiangsu Ronghui Environmental Protection Equipment Co., Ltd.

Figure 15: Jiangsu Ronghui Environmental Protection Equipment Co., Ltd.

A very good dye product that avoids pollution from the production process achieve the integration of enterprise production and biotechnology to solve environmental problems from the root causes is needed.

Environmental Protection Bureau of Yantai

Environmental Protection Bureau of Yantai

Figure 16: Environmental Protection Bureau of Yantai

Try to make good products that can protect the ecology, protect the environment and meet the needs of the public, so as to achieve a win-win situation of economic development and environmental protection.

3.3 Chinese traditional Plant Dye Workshop

Tie-dyeing set off a fashion craze because of its gorgeous and simple design. However, due to the promulgation of the national policy on environmental protection, the tie-dyeing industry is becoming extinct, and because of the serious aging of the employees, the tie-dyeing technology has the risk of losing generations. At the same time, the key point of tie-dyeing technology is to use plants for dyeing, but in the production process of tie-dyeing, plant dyes have low productivity, raw materials are more expensive and rare, which makes more convenient and fast chemical dyeing to replace plant dyeing in a large area. Mr.Tony was born in the laboratory. In order to synthesize natural dyes by engineering yeast, it is necessary to solve the problems of complex preparation process and low purity of dyes in the traditional tie-dyeing process, so as to realize the mass production of natural dyes. In the follow-up, the team needs to explore the optimal dyeing conditions and color mixing experiments of the dyes, so as to provide the possibility for showing a variety of colors in the tie-dyeing process. At the same time, it is also very important to realize the quantitative analysis of plant dyes and clarify the chemical composition, optimal dosage and biochemical principles of dyes and auxiliaries in the dyeing process through experiments. so as to help tie-dyeing, a traditional handicraft industry on a large scale. Let the tie-dyeing process which strongly depends on traditional dyes go further and grow stronger.

Lan dyeing workshop

Lan dyeing workshop

Figure 17: Lan dyeing workshop

The development of any dye needs to clarify the chemical composition and optimal dosage of dyes and auxiliaries in the dyeing process, as well as the biochemical principles in the dyeing process, so as to help more craftsmen accurately grasp the amount of dyes.

Dali Puzhen Museum

Dali Puzhen Museum

Figure 18: Dali Puzhen Museum

Mr.Tony is needed to solve the problems of complex dye preparation process and low purity of dyes used in traditional tie-dyeing process, so as to achieve mass production of natural dyes, to help more craftsmen to achieve large-scale, better promotion of traditional technology.

Lanxu ancient tie-dye experience Hall

It is necessary to realize the quantitative analysis of plant dyes, clarify the chemical composition of dyes and dyeing aids in the dyeing process, the optimal dosage and the biochemical principles in the dyeing process, so as to provide solutions to the traditional processes facing the crisis of extinction.

Lanxu ancient tie-dye experience Hall

Figure 19: Lanxu ancient tie-dye experience Hall

Unbelievable! You have done such a thorough and detailed job just in the step of identifying the problem!! Let's get to the second part: 'Formulate implementation plan' right away!

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