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− | {{IGEM_TopBar}}
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− | {{Shanghai_HS_United}}
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| <html> | | <html> |
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− | <div class="column full_size"> | + | <link rel="stylesheet" type="text/css" |
| + | href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_HS_United/StyleCSS&action=raw&ctype=text/css"/> |
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− | <h1>Notebook</h1> | + | <div class="flavone-lab-content"> |
− | <p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p> | + | <!--navbar--> |
| + | <div class="navbar"> |
| + | <a class="logo" href="https://2021.igem.org/Team:Shanghai_HS_United"> |
| + | <img src="https://static.igem.org/mediawiki/2021/c/c1/T--Shanghai_HS_United--logo.png" alt=""> |
| + | </a> |
| | | |
− | </div> | + | <!--nav--> |
− | <div class="clear"></div> | + | <ul class="list"> |
| + | <!--Team--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Team">Team</a> |
| + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Team">Team Members</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Attributions">Attributions</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Collaborations">Collaboration</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Partnership">Partnership</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
| | | |
| + | <!--Modeling--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Model">Modeling</a> |
| + | </li> |
| | | |
| + | <!--Entrepreneurship--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Entrepreneurship">Entrepreneurship</a> |
| + | </li> |
| | | |
− | <div class="column two_thirds_size"> | + | <!--Implementation--> |
− | <h3>What should this page have?</h3> | + | <li class="item"> |
− | <ul>
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Implementation">Implementation</a> |
− | <li>Chronological notes of what your team is doing.</li>
| + | </li> |
− | <li> Brief descriptions of daily important events.</li>
| + | |
− | <li>Pictures of your progress. </li>
| + | |
− | <li>Mention who participated in what task.</li>
| + | |
− | </ul>
| + | |
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− | </div> | + | <!--Human Practice--> |
| + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Human_Practices">Human Practice</a> |
| + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Human_Practices">Integrated Human |
| + | Practice</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Communication">Communication</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Fundraising">Fundraising</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
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− | <div class="column third_size"> | + | <!--Parts--> |
− | <div class="highlight decoration_A_full"> | + | <li class="item"> |
− | <h3>Inspiration</h3> | + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/PartsCollection">Parts</a> |
− | <p>You can see what others teams have done to organize their notes:</p> | + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Parts_Collection">Parts |
| + | Collection</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Engineering">Engineering</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
| | | |
− | <ul> | + | <!--Project--> |
− | <li><a href="https://2018.igem.org/Team:Munich/Notebook">2018 Munich</a></li> | + | <li class="item active"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Description">Project</a> |
| + | <div class="child-nav"> |
| + | <ul class="child-list"> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Description">Description</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Experiments">Experiments</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Results">Result</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/ProofOfConcept">Proof of Concept</a> |
| + | </li> |
| + | <li class="child-item active"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Notebook">Notebook</a> |
| + | </li> |
| + | <li class="child-item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United/Safety">Safety</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| + | </li> |
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− | <li><a href="https://2019.igem.org/Team:Georgia_State/Notebook">2019 Georgia State</a></li> | + | <!--Home--> |
− | <li><a href="https://2019.igem.org/Team:Newcastle/Notebook">2019 Newcastle</a></li> | + | <li class="item"> |
| + | <a href="https://2021.igem.org/Team:Shanghai_HS_United">Home</a> |
| + | </li> |
| + | </ul> |
| + | </div> |
| | | |
| + | <!--图片--> |
| + | <div class="page-header"> |
| + | <img src="https://static.igem.org/mediawiki/2021/9/97/T--Shanghai_HS_United--bg_1.png" alt=""> |
| + | <h1>Notebook</h1> |
| + | </div> |
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− | <li><a href="https://2020.igem.org/Team:IISER-Pune-India/Notebook">2020 IISER Pune India</a></li> | + | <!--内容--> |
− | <li><a href="https://2020.igem.org/Team:Lund/Notebook">2020 Lund</a></li> | + | <div class="content-small"> |
− | <li><a href="https://2020.igem.org/Team:NOVA_LxPortugal/Notebook">2020 NOVA LxPortugal</a></li> | + | <section class="article p-t-54 p-b-54"> |
− | <li><a href="https://2020.igem.org/Team:RDFZ-China/NoteBook">2020 RDFZ China</a></li>
| + | <img src="https://static.igem.org/mediawiki/2021/d/de/T--Shanghai_HS_United--img_notebook_1.jpg" alt="" style="width: 70%"> |
− | </ul>
| + | |
− | </div>
| + | |
− | </div>
| + | |
| | | |
| + | <section> |
| + | <h1 class="title blue bold">July</h1> |
| + | <p><b>25<sup>th</sup>-26<sup>th</sup></b> We finalized our team members and elected the team leader, design |
| + | the team name, logo, uniforms, and perimeters. We conducted laboratory safety training and brief |
| + | schedule analysis.</p> |
| + | <p><b>28<sup>th</sup></b> We made culture medium and buffer A and B, and done the PCR amplification.</p> |
| + | <p><b>29<sup>th</sup></b> We did the plasmid conversion, spread bacterial colony, and shook Escherichia |
| + | coli.</p> |
| + | <p><b>30<sup>th</sup></b> We collected the linear no-load vector, which was detected by gel electrophoresis |
| + | and recovered by gel extraction kit.</p> |
| + | <p><b>31<sup>st</sup></b> We cultured and then centrifuged E. coli, to obtain the plasmid for further |
| + | preservation.</p> |
| + | </section> |
| + | |
| + | <section> |
| + | <h1 class="title blue bold">August</h1> |
| + | <p><b>1<sup>st</sup></b> We did the restriction enzyme digestion, prepared the gel for the gel |
| + | electrophoresis, transformed the plasmid, and poured the plates.</p> |
| + | <p><b>2<sup>nd</sup></b> We did the inoculation, prepared LB culture media, and the protein induction.</p> |
| + | <p><b>3<sup>rd</sup></b> We purified the protein through Ni-chelating affinity chromatography.</p> |
| + | <p><b>19<sup>th</sup>-27<sup>th</sup></b> We conducted the function test to analyze the protein activity. |
| + | </p> |
| + | </section> |
| + | |
| + | <br> |
| + | <br> |
| + | <img src="https://static.igem.org/mediawiki/2021/0/00/T--Shanghai_HS_United--img_notebook_2.jpg" alt="" style="width: 60%"> |
| + | </section> |
| + | </div> |
| + | |
| + | <!--Footer--> |
| + | <div class="footer"> |
| + | <div class="content"> |
| + | <hr> |
| + | <span>Email: xiaowenxuan0526@gmail.com; caroline.niujl@gmail.com</span> |
| + | <span>Wechat Official Account: FlavoneLabRouse</span> |
| + | </div> |
| + | </div> |
| + | </div> |
| </html> | | </html> |