|
|
| Line 117: |
Line 117: |
| | the project which motivated us to seek answers and incorporate them in our project. | | the project which motivated us to seek answers and incorporate them in our project. |
| | Along with this they also provided with valuable suggestions which have been | | Along with this they also provided with valuable suggestions which have been |
| − | incorporated as and when required. | + | incorporated as and when required. See a brief documentation of OUR INTERACTIONS during the course of this project. |
| | </p> | | </p> |
| | <br> | | <br> |
| Line 157: |
Line 157: |
| | </div> | | </div> |
| | </div> | | </div> |
| − | <div class="container-2"> | + | |
| − | <h1 class="text">HP Timeline</h1>
| + | <iframe src="https://static.igem.org/mediawiki/2021/a/ad/T--IISER_Bhopal--Human_Practices--Integrated_HP-our_interactions.pdf" height="800" width="100%"></iframe> |
| − | <br>
| + | |
| − | <center>
| + | |
| − | <h2>Doctor Sanjeev Shukla
| + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/1.jpg" style="width: 30%; height:30%" alt="">
| + | |
| − | <h2>22nd March 2021
| + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Dr. Sanjeev Shukla is the Dean of Research and Development at IISER Bhopal
| + | |
| − | (2018-present) and associate professor in the department of Biological sciences and his
| + | |
| − | research work mainly focuses on cancer and the role of epigenetics in carcinogenesis.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>Through this meeting we wanted to understand about properties of cancer cells which we
| + | |
| − | can use against them and so engineering the bacteria accordingly.
| + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>All cells express a basal level of lactate concentration. To construct a kill switch for
| + | |
| − | our bacteria we need a lactate inducible promoter which is concentration specific. </li>
| + | |
| − | <br>
| + | |
| − | <li>Bacteria and tumor growth kinetics are different (5 hours and 20 hours respectively).
| + | |
| − | The bacteria will end up consuming nutrients of the media on co-culturing both bacteria
| + | |
| − | and cancer cells, causing tumor cell death inevitably. We need to ensure that the
| + | |
| − | activity of TRAIL and Smac are causing that activity. </li>
| + | |
| − | <br>
| + | |
| − | <li>He also suggested that a hypoxia chamber for anaerobic bacteria culture can be created
| + | |
| − | by using chemicals like CoCl2.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <h2>Integration
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>For ensuring activity of TRAIL and Smac on cancer cells, we decided not to co-culture
| + | |
| − | the bacteria and cancer cells. We planned to isolate the fusion protein and look for the
| + | |
| − | cytotoxicity of the same on the cancer cells.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | </div>
| + | |
| − | </div> | + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Doctor Partho Sarothy Ray
| + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/2.jpg" style="width: 20%; height:20%" alt="">
| + | |
| − | <h2>6th June 2021
| + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Dr. Partho Sarothi Ray is an associate professor in the department of Biological
| + | |
| − | Sciences at IISER Kolkata. His research interest lies in RNA-binding protein and
| + | |
| − | miRNA-mediated regulation of gene expression in inflammation and cancer, computational
| + | |
| − | and experimental biology of gene expression networks, drug designing for cellular
| + | |
| − | imaging and photo-dynamic therapy, molecular evolution.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>By meeting him we wanted a brief review of our project as well as wanted to know about
| + | |
| − | the possible mechanisms for activation of the kill switch.
| + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>He made us realize that the effectiveness of our solution is limited mostly to hypoxic
| + | |
| − | and solid tumors, since all tumors are not necessarily hypoxic and the bacteria
| + | |
| − | (Bifidobacterium longum) grows in anaerobic conditions.
| + | |
| − | </li><br>
| + | |
| − | <li>He suggested doing the proof of concept in E.Coli as it is fit for almost any promoter
| + | |
| − | and rbs.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>As every bacteria expressing proteins are not secreted outside the cells, he suggested
| + | |
| − | going for a lysis approach in which contents of the bacteria are released outside into
| + | |
| − | the tumor cells.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>As every bacteria expressing proteins are not secreted outside the cells, he suggested
| + | |
| − | going for a lysis approach in which contents of the bacteria are released outside into
| + | |
| − | the tumor cells.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>Use of fusion peptide might not be a good idea, as it can impact folding of the proteins
| + | |
| − | which might decrease their effectiveness.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>Tumor milieu is unstable in terms of extracellular matrix so biofilm formation won’t be
| + | |
| − | taking place there.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>As all tumors are not accessible, we should go for intravenous administration of the
| + | |
| − | bacteria instead of intratumoral, when it comes to drug designing. </li>
| + | |
| − | <br>
| + | |
| − | <h2>Integration
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>We decided to eliminate the plan of quorum sensing and incorporated an AND gate system
| + | |
| − | for double proofing.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>We planned to use both Lactate inducible promoter and Hypoxia inducible promoter for the
| + | |
| − | AND gate.
| + | |
| − | </li><br>
| + | |
| − | <li>For proof of concept we plan to use E.Coli DH5 alpha strain as it is a facultative
| + | |
| − | anaerobe.
| + | |
| − | </li><br>
| + | |
| − | <li>This meeting formed the motivation for structural modelling of the native sequences and
| + | |
| − | of fusion protein to ensure that folding remains unaffected.
| + | |
| − | </li><br>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Shrestha Rath
| + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/3.jpg" style="width: 30%; height:30%" alt="">
| + | |
| − | <h2>9th June 2021
| + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | She is a previous iGEM IISER Bhopal team member and is currently the after iGEM Regional
| + | |
| − | Ambassadors Coordinator of Asia.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>We arranged a meeting with her to understand the safety considerations regarding our
| + | |
| − | project.
| + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>She pointed out the problem of Horizontal gene transfer when it comes to working with
| + | |
| − | bacterias.
| + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>Risks associated with injecting the bacteria- they can elicit SEPSIS, and other
| + | |
| − | immunogenic responses so we need to look for best possible ways to avoid such problems.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>She suggested looking for iGEM and outside iGEM projects pertaining to Bacteriotherapy
| + | |
| − | to check for progressivity and novelty in our project.
| + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Doctor Vikas Jain
| + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/4.jpg" style="width: 20%; height:20%" alt="">
| + | |
| − | <h2>17th June 2021
| + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Doctor Vikas Jain is an associate professor at IISER Bhopal (2015- present) in the
| + | |
| − | Department of Biological Sciences and his work involves basic and applied research in
| + | |
| − | bacteriophages and phage infection in Mycobacterium and thus developing phage therapy
| + | |
| − | for tuberculosis.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>After validating an idea about the effectiveness of a fusion peptide of Smac and TRAIL we
| + | |
| − | aimed to build a robust and effective fusion protein.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>He suggested the use of serine and glycine linkers.
| + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>He suggested using Histidine tag at the C terminus, after or before the linker.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>He helped us develop a cloning strategy in which 7 base spacers are necessary after the
| + | |
| − | ribosome binding site can add 4 CCCC before ATG.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>Kill switch can be expressed as an operon system. He suggested inserting a Lambda
| + | |
| − | repressor to balance the sensitivity of the kill switch and hence protecting the
| + | |
| − | bacteria while traversing through the system to the tumor site.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | | + | |
| − | <h2>Integration
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>The kill switch and the fusion protein were constructed as guided by Doctor Vikas Jain.
| + | |
| − | </li>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Soumodeep Sarkar
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/5.jpg" style="width: 30%; height:30%" alt="">
| + | |
| − | <h2>19th June 2021
| + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Soumodeep Sarkar is iGEM Ambassador to Asia after iGEM and is the host of iGEM TV Show-
| + | |
| − | SynBioFlix.
| + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>We wanted to understand about safety policies of iGEM and hence we attended his workshop.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>Team members’ safety should be primary when it comes to performing experimental work,
| + | |
| − | followed by people the project we are doing for, animals and the environment.
| + | |
| − | | + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>Certain organisms and parts are included in the white list which signifies organisms
| + | |
| − | safe to use and allowed by the iGEM committee.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>When developing a therapeutic project one should consider safety of parts, safe wet lab
| + | |
| − | work, and maximising use of organisms and parts on white list rather than those which
| + | |
| − | are not on the list.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Doctor Sushma Agrawal
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/6.png" style="width: 30%; height:30%" alt="">
| + | |
| − | <h2>24th June 2021
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Dr. Sushma Agrawal is an associate professor in the Department of Radiotherapy, at
| + | |
| − | Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India. She
| + | |
| − | specializes in radiology and oncology. Her educational qualifications include M.B.B.S in
| + | |
| − | General Medicine, M.D. and D.N.B in radiotherapy.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>By meeting with her we wanted a review of the project and as she is a doctor, she helped
| + | |
| − | us understand the applicative aspect of our project.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>Most of the access to the treatment comes down to the cost and effectiveness and
| + | |
| − | associated side effects of it.
| + | |
| − | | + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>‘Solid tumors when grow big, their center becomes hypoxic. In clinical practice some
| + | |
| − | tumors undergo surgery and then after the tumor is out then we give chemotherapy’.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>Side effects of the treatment depend on the modality of the treatment. ‘If we are using
| + | |
| − | chemotherapy then there is myelosuppression, loss of hair, diarrhoea. And in targeted
| + | |
| − | therapy it depends upon which targeted therapy we are using, but like basically,it is
| + | |
| − | dermatitis, diarrhoea. Next type of therapy is immunotherapy which has a vast plethora
| + | |
| − | of side effects, and depending upon the organ which is affected,and side effects can
| + | |
| − | involve any of the organs and radiotherapy, side effects depend on where you are
| + | |
| − | irradiating. If on abdomen then diarrhea, if on chest then lung toxicity.’
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>Radiotherapy doesn’t work in hypoxic conditions, so we can use our approach to destroy
| + | |
| − | hypoxic cells and increase radiosensitivity of the tumor.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>An interview with a Cancer Survivor
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/7.png" style="width: 30%; height:30%" alt="">
| + | |
| − | <h2>4th July 2021
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | The person had a history of Osteosarcoma which got detected in the first stage. The
| + | |
| − | identity of the person has been kept anonymous and has been referred as ‘the person’ for
| + | |
| − | the same.
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>We had problems in figuring out methods to administer our drug, thus we wanted her
| + | |
| − | insights over the same.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>A pain for 10 days led to diagnosis of osteosarcoma of stage I in the right fibula with
| + | |
| − | a CT scan.
| + | |
| − | | + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>Six weeks of chemotherapy followed by amputation surgery as well as six months of
| + | |
| − | chemotherapy were administered to this person in a span of 10 months costing about 5 lac
| + | |
| − | rupees.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>The treatment affected almost all aspects of life and during the treatment the person
| + | |
| − | encountered side effects like ulcers, vomiting, hair loss, tiredness, decreased
| + | |
| − | appetite.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>The person, now a cancer survivor, believes in educating people about cancer and its
| + | |
| − | treatments for making them mentally ready to undergo therapy in case they suffer from
| + | |
| − | cancer, this will help reduce anxiety in the family and will make them come out stronger
| + | |
| − | against the fight of cancer. Also, a psychologist in the department of cancer care might
| + | |
| − | help people to face the ordeal.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | | + | |
| − | <li>The person gave important advice - ‘If you feel any pain, get it checked, do not leave
| + | |
| − | it’.
| + | |
| − | </li>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Doctor Rajesh K. Murarka
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/9.jpg" style="width: 20%; height:20%" alt="">
| + | |
| − | <h2>24th August 2021
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>Dr. Rajesh K. Murarka is an associate professor at the department of Chemistry at IISER
| + | |
| − | Bhopal and specializes in Computational Biophysics and Chemistry. His research focuses
| + | |
| − | on understanding conformational and structural dynamics of biological macromolecules and
| + | |
| − | their interactions with the surrounding solvent environments.
| + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>To discuss over how to start working with MD Simulations
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>He suggested going for a coarse grained simulation because atomistic MD simulations will
| + | |
| − | take a lot of time and hence not feasible. We are currently aided by one of his Ph.D
| + | |
| − | students for the same. ze and type of tv
| + | |
| − | </li>>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Doctor Maciej Maslyk
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/10.png" style="width: 20%; height:20%" alt="">
| + | |
| − | <h2>24th September 2021
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Dr. Maciej Maslyk is a professor in the department of Molecular Biology in the Institute
| + | |
| − | of Biological Sciences at The John Paul II Catholic University, Lublin (KUL) in Poland.
| + | |
| − | His research interests are into molecular biology, bioactive compounds, biotechnology &
| + | |
| − | antimicrobial agents.
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>As we incorporated most of the work of him and his colleagues in our project we had some
| + | |
| − | doubts regarding the synthesis of fusion peptides and other experimental aspects.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>As we incorporated most of the work of him and his colleagues in our project we had some
| + | |
| − | doubts regarding the synthesis of fusion peptides and other experimental aspects.
| + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>We earlier thought of incorporating whole Smac cds but that complicates the process of
| + | |
| − | cleavage needed for exposition of the AVPI consensus. When using a full length protein,
| + | |
| − | its activity will depend on the additional processes and its efficiency.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>
| + | |
| − | His paper showed tumor regrowth when TRAIL alone was used as it acts in the feedback
| + | |
| − | way.
| + | |
| − | </li> <br>
| + | |
| − | | + | |
| − | <li>Tat protein is a good candidate for cell penetrating peptide.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | | + | |
| − | <h2>Integration
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>As he suggested we decided to go for a fusion protein rather than TRAIL and Smac
| + | |
| − | separately and only the 7 amino acids of Smac were used which simplified its activity in
| + | |
| − | the fusion peptide. For cell penetrating peptides his work inspired us to go for ones
| + | |
| − | with better internalisation processes documented other than Tat, hence we went for Octa
| + | |
| − | Arginine for assisting in internalization of Smac.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Doctor Padma Devrajan
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/11.png" style="width: 20%; height:20%" alt="">
| + | |
| − | <h2>4th October 2021
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Doctor Padma Devrajan is the Coordinator of the World Bank Technical Education Quality
| + | |
| − | Improvement Programme (TEQIP) at Institute of Chemical Technology, Mumbai. She is the
| + | |
| − | professor in Pharmacy and former Head of Department of Pharmaceutical Sciences and
| + | |
| − | Technology. Her work focuses on drug delivery systems for human and veterinary
| + | |
| − | healthcare.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>Meeting with her helped us understand about the formulation of the intravenous injection
| + | |
| − | of Bifidobacteria.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>The concern of bacteria being administered in veins was assured after the paper
| + | |
| − | discussing the safety of intravenous administration was studied by us.
| + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>Any soluble substance should be isotonic to the blood or plasma which has a molarity of
| + | |
| − | about 0.3 M of solutes.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>Bifidobacterium longum will not be stable in the suspension medium so it needs to be
| + | |
| − | freeze dried.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>To produce viable bacteria and stable products the freeze dried system is converted into
| + | |
| − | powder and cryoprotectants like Trehalose are added to avoid stressing on bacteria
| + | |
| − | during the freeze drying process.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>After freeze drying check for OD of bacteria as well as ensure homogenous distribution
| + | |
| − | of the bacteria in the freeze-dried powder.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>Richa Ahuja (Abiogenesis Clinpharm)
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/12.jpg" style="width: 50%; height:50%" alt="">
| + | |
| − | <h2>20th October 2021
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Richa Ahuja is the Senior Business Development Executive at Abiogenesis Clinpharm and is
| + | |
| − | an experienced professional in the clinical research industry. Abiogenesis Clinpharm is
| + | |
| − | a Clinical Research Organization (CRO) with expertise in providing Clinical trials of
| + | |
| − | drugs, medical devices, nutraceuticals and other products.
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>Meeting with her helped us to understand what needs to be done to start with human
| + | |
| − | trials.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>She suggested going to clinical trials once the trials and experiments with cell lines
| + | |
| − | and then animal models are done. She helped us connect to Rodenta Bioserve for
| + | |
| − | preclinical studies.
| + | |
| − | | + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>The organisation provides trials from phase I to phase IV and she suggested going till
| + | |
| − | phase II and then we can go for phase III after approval of DCGI.
| + | |
| − | | + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>As per her suggestions, side effects of a drug can manifest within 24 hours, so the
| + | |
| − | group under study needs to be observed for 2 to 24 hours to check for any side effects.
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <center>
| + | |
| − | <h2>
| + | |
| − | Doctor Kalle Anand Kumar (Rodenta Bioserve)
| + | |
| − | | + | |
| − | </h2>
| + | |
| − | <img class="Images" src="assets/img/ihp/13.jpg" style="width: 20%; height:20%" alt="">
| + | |
| − | <h2>17th June 2021
| + | |
| − | </h2>
| + | |
| − | </center>
| + | |
| − | <br>
| + | |
| − | <h2>Getting acquainted</h2>
| + | |
| − | <br>
| + | |
| − | <div class="p-linklist" style="text-align: left;">
| + | |
| − | <p>
| + | |
| − | Doctor Kalle Anand Kumar is the Chief scientific officer and a founding member of
| + | |
| − | Rodenta Bioserve. Rodenta Bioserve is a pre clinical research organisation which
| + | |
| − | provides animal models and protocols for various research studies and assists
| + | |
| − | pharma/graduate/postgraduate student research projects, private companies.
| + | |
| − | | + | |
| − | | + | |
| − | </p>
| + | |
| − | | + | |
| − | <br>
| + | |
| − | <h2>Purpose</h2>
| + | |
| − | <br>
| + | |
| − | <p>We met him to gain insights about how to proceed with pre-clinical trials as they will
| + | |
| − | form the basis for most of the invivo studies for various solid tumors.
| + | |
| − | | + | |
| − | </p>
| + | |
| − | <br>
| + | |
| − | <h2>Discussion
| + | |
| − | </h2>
| + | |
| − | <br>
| + | |
| − | <li>He guided us for transition from in vitro studies to the in vivo studies and aided us
| + | |
| − | with a protocol for a brief plan of action.
| + | |
| − | | + | |
| − | </li><br>
| + | |
| − | <li>He suggested the use of Rabbit (New Zealand White species) as a model organism to study
| + | |
| − | the toxic effects of the fusion protein, if any.
| + | |
| − | | + | |
| − | </li>
| + | |
| − | <br>
| + | |
| − | <li>He helped us form a brief protocol for both the efficacy and toxicity studies of the
| + | |
| − | fusion protein.
| + | |
| − | | + | |
| − | </div>
| + | |
| − | </div>
| + | |
| − | | + | |
| − | | + | |
| − | | + | |
| − | <div class="container-2">
| + | |
| − | <h1 class="text" style="width: 50%; ">Timeline in order
| + | |
| − | </h1>
| + | |
| − | <p> <a href="https://docs.google.com/spreadsheets/d/12MznxDwi7SiqRe_7PB5Hp1gNaTOzamEvmpNoB0v0B_0/edit#gid=300905365 ">
| + | |
| − | https://docs.google.com/spreadsheets/d/12MznxDwi7SiqRe_7PB5Hp1gNaTOzamEvmpNoB0v0B_0/edit#gid=300905365</a></p>
| + | |
| − | | + | |
| − | </div>
| + | |
| | | | |
| | </div> | | </div> |
