Difference between revisions of "Team:Vilnius-Lithuania/Results"
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<td>Technical grade <i>p</i>-coumaric acid</td> | <td>Technical grade <i>p</i>-coumaric acid</td> | ||
<td>Technical grade <i>p</i>-coumaric acid was dissolved in water for reference chromatogram and other specifications.</td> | <td>Technical grade <i>p</i>-coumaric acid was dissolved in water for reference chromatogram and other specifications.</td> | ||
| − | <td> | + | <td><a download="" href="https://static.igem.org/mediawiki/2021/e/e9/T--Vilnius-Lithuania--p-coum_pure.PDF"><b>link</b></a> </td> |
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<td>TAL1 lysate </td> | <td>TAL1 lysate </td> | ||
<td>First enzyme Tyrosine ammonia lyase (TAL) in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium. Culture was lysed to check for compounds inside cells.</td> | <td>First enzyme Tyrosine ammonia lyase (TAL) in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium. Culture was lysed to check for compounds inside cells.</td> | ||
| − | <td> | + | <td><a download="" href="https://static.igem.org/mediawiki/2021/a/a7/T--Vilnius-Lithuania--TAL_lys.PDF"><b>link</b></a> </td> |
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<td>TAL1 LB medium</td> | <td>TAL1 LB medium</td> | ||
<td>First enzyme Tyrosine ammonia lyase (TAL) in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium. Only LB medium was subjected to HPLC-MS analysis.</td> | <td>First enzyme Tyrosine ammonia lyase (TAL) in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium. Only LB medium was subjected to HPLC-MS analysis.</td> | ||
| − | <td> | + | <td><a download="" href="https://static.igem.org/mediawiki/2021/a/a4/T--Vilnius-Lithuania--TAL_nonlys.PDF"><b>link</b></a> </td> |
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</tr> | </tr> | ||
<tr> | <tr> | ||
<td>DH5ɑ with <i>p</i>-coumaric acid</td> | <td>DH5ɑ with <i>p</i>-coumaric acid</td> | ||
<td>Only DH5ɑ without any plasmid cells were grown in LB medium with supplied <i>p</i>-coumaric acid. </td> | <td>Only DH5ɑ without any plasmid cells were grown in LB medium with supplied <i>p</i>-coumaric acid. </td> | ||
| − | <td> | + | <td></td><a download="" href="https://static.igem.org/mediawiki/2021/e/e9/T--Vilnius-Lithuania--p-coum_pure.PDF"><b>link</b></a> </td> |
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</tr> | </tr> | ||
<tr> | <tr> | ||
<td>LB with <i>p</i>-coumaric acid</td> | <td>LB with <i>p</i>-coumaric acid</td> | ||
<td>LB medium with <i>p</i>-coumaric acid.</td> | <td>LB medium with <i>p</i>-coumaric acid.</td> | ||
| − | <td> | + | <td><a download="" href="https://static.igem.org/mediawiki/2021/7/7a/T--Vilnius-Lithuania--LB_with_p-coum.PDF"><b>link</b></a> </td> |
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<td>Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid without supplied <i>p</i>-coumaric acid to LB medium.</td> | <td>Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid without supplied <i>p</i>-coumaric acid to LB medium.</td> | ||
<td><a download="" href="https://static.igem.org/mediawiki/2021/c/cf/T--Vilnius-Lithuania--GS1_LB_without_p-coum.PDF"><b>link</b></a> </td> | <td><a download="" href="https://static.igem.org/mediawiki/2021/c/cf/T--Vilnius-Lithuania--GS1_LB_without_p-coum.PDF"><b>link</b></a> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>GS1 lysate with <i>p</i>-coumaric acid</td> | ||
| + | <td>Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid without supplied <i>p</i>-coumaric acid to LB medium. </td> | ||
| + | <td><a download="" href="https://static.igem.org/mediawiki/2021/d/d7/T--Vilnius-Lithuania--GS1_lys.PDF"><b>link</b></a> </td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>GS1 LB medium with <i>p</i>-coumaric acid</td> | <td>GS1 LB medium with <i>p</i>-coumaric acid</td> | ||
<td>Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium.</td> | <td>Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium.</td> | ||
| − | <td><a href="https://static.igem.org/mediawiki/2021/b/b7/T--Vilnius-Lithuania--GS1_LB_with_p-coum.PDF | + | <td><a download="" href="https://static.igem.org/mediawiki/2021/b/b7/T--Vilnius-Lithuania--GS1_LB_with_p-coum.PDF"><b>link</b></a> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>GSG LB medium with <i>p</i>-coumaric acid</td> | <td>GSG LB medium with <i>p</i>-coumaric acid</td> | ||
<td>Fusion protein (4CL and CHS) construct with GSG linker in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium. Culture was lysed to check for compounds inside cells.</td> | <td>Fusion protein (4CL and CHS) construct with GSG linker in pTRKH2 plasmid with supplied <i>p</i>-coumaric acid to LB medium. Culture was lysed to check for compounds inside cells.</td> | ||
| − | <td><a href="https://static.igem.org/mediawiki/2021/5/52/T--Vilnius-Lithuania--GSG.PDF | + | <td><a download="" href="https://static.igem.org/mediawiki/2021/5/52/T--Vilnius-Lithuania--GSG.PDF"><b>link</b></a> </td> |
</tr> | </tr> | ||
</tbody></table> | </tbody></table> | ||
Revision as of 08:54, 20 October 2021
RESULTS
Headline 1
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus ac enim id metus rutrum blandit sed non dolor. Pellentesque feugiat odio eu imperdiet rutrum. Duis consectetur porttitor enim, id elementum nibh tempus in. Nulla ut massa rutrum, ullamcorper dui et, posuere velit. Cras viverra, tortor at porta pulvinar, libero eros pulvinar orci, sed pulvinar neque felis eget dui. Fusce laoreet libero vitae nunc hendrerit consequat. Nunc eget bibendum turpis. Vestibulum pulvinar interdum mauris nec congue. Etiam id nunc ac risus dictum semper sed in nisl.
| Name | Description | File |
|---|---|---|
| Technical grade p-coumaric acid | Technical grade p-coumaric acid was dissolved in water for reference chromatogram and other specifications. | link |
| TAL1 lysate | First enzyme Tyrosine ammonia lyase (TAL) in pTRKH2 plasmid with supplied p-coumaric acid to LB medium. Culture was lysed to check for compounds inside cells. | link |
| TAL1 LB medium | First enzyme Tyrosine ammonia lyase (TAL) in pTRKH2 plasmid with supplied p-coumaric acid to LB medium. Only LB medium was subjected to HPLC-MS analysis. | link |
| DH5ɑ with p-coumaric acid | Only DH5ɑ without any plasmid cells were grown in LB medium with supplied p-coumaric acid. | link |
| LB with p-coumaric acid | LB medium with p-coumaric acid. | link |
| LB without p-coumaric acid | LB medium without p-coumaric acid. | link |
| GS1 LB medium without p-coumaric acid | Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid without supplied p-coumaric acid to LB medium. | link |
| GS1 lysate with p-coumaric acid | Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid without supplied p-coumaric acid to LB medium. | link |
| GS1 lysate with p-coumaric acid | Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid without supplied p-coumaric acid to LB medium. | link |
| GS1 LB medium with p-coumaric acid | Fusion protein (4CL and CHS) construct with GGGGS linker in pTRKH2 plasmid with supplied p-coumaric acid to LB medium. | link |
| GSG LB medium with p-coumaric acid | Fusion protein (4CL and CHS) construct with GSG linker in pTRKH2 plasmid with supplied p-coumaric acid to LB medium. Culture was lysed to check for compounds inside cells. | link |
- Item 1
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- Item 3
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- Item 3
Cras ultrices eu massa vitae congue. Vestibulum arcu enim, congue id lacus at, laoreet fringilla tortor. Praesent libero nunc, maximus vel suscipit nec, fringilla sed augue. Praesent porttitor vehicula efficitur. Aenean ac bibendum lectus. Praesent vestibulum velit ut nunc accumsan, non molestie erat pretium. In a ante vulputate, semper sapien at, tristique erat. Morbi vitae euismod eros, at pulvinar erat. Vivamus auctor arcu sed tellus egestas, vitae imperdiet eros viverra. Nulla molestie sapien vitae ipsum pulvinar dictum. Sed sit amet dolor a neque dictum malesuada et nec lectus. Aenean tristique ornare nisl, at imperdiet magna placerat eget. Suspendisse ut blandit elit, sit amet iaculis ex. Nam vel varius velit. Aenean pretium scelerisque enim, quis mollis nunc finibus eu.
Cras ultrices eu massa vitae congue. Vestibulum arcu enim, congue id lacus at, laoreet fringilla tortor. Praesent libero nunc, maximus vel suscipit nec, fringilla sed augue. Praesent porttitor vehicula efficitur. Aenean ac bibendum lectus. Praesent vestibulum velit ut nunc accumsan, non molestie erat pretium. In a ante vulputate, semper sapien at, tristique erat. Morbi vitae euismod eros, at pulvinar erat. Vivamus auctor arcu sed tellus egestas, vitae imperdiet eros viverra. Nulla molestie sapien vitae ipsum pulvinar dictum. Sed sit amet dolor a neque dictum malesuada et nec lectus. Aenean tristique ornare nisl, at imperdiet magna placerat eget. Suspendisse ut blandit elit, sit amet iaculis ex. Nam vel varius velit. Aenean pretium scelerisque enim, quis mollis nunc finibus eu.
Cras ultrices eu massa vitae congue. Vestibulum arcu enim, congue id lacus at, laoreet fringilla tortor. Praesent libero nunc, maximus vel suscipit nec, fringilla sed augue. Praesent porttitor vehicula efficitur. Aenean ac bibendum lectus. Praesent vestibulum velit ut nunc accumsan, non molestie erat pretium. In a ante vulputate, semper sapien at, tristique erat. Morbi vitae euismod eros, at pulvinar erat. Vivamus auctor arcu sed tellus egestas, vitae imperdiet eros viverra. Nulla molestie sapien vitae ipsum pulvinar dictum. Sed sit amet dolor a neque dictum malesuada et nec lectus. Aenean tristique ornare nisl, at imperdiet magna placerat eget. Suspendisse ut blandit elit, sit amet iaculis ex. Nam vel varius velit. Aenean pretium scelerisque enim, quis mollis nunc finibus eu.
Headline 2
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus ac enim id metus rutrum blandit sed non dolor. Pellentesque feugiat odio eu imperdiet rutrum. Duis consectetur porttitor enim, id elementum nibh tempus in. Nulla ut massa rutrum, ullamcorper dui et, posuere velit. Cras viverra, tortor at porta pulvinar, libero eros pulvinar orci, sed pulvinar neque felis eget dui. Fusce laoreet libero vitae nunc hendrerit consequat. Nunc eget bibendum turpis. Vestibulum pulvinar interdum mauris nec congue. Etiam id nunc ac risus dictum semper sed in nisl.
Cras ultrices eu massa vitae congue. Vestibulum arcu enim, congue id lacus at, laoreet fringilla tortor. Praesent libero nunc, maximus vel suscipit nec, fringilla sed augue. Praesent porttitor vehicula efficitur. Aenean ac bibendum lectus. Praesent vestibulum velit ut nunc accumsan, non molestie erat pretium. In a ante vulputate, semper sapien at, tristique erat. Morbi vitae euismod eros, at pulvinar erat. Vivamus auctor arcu sed tellus egestas, vitae imperdiet eros viverra. Nulla molestie sapien vitae ipsum pulvinar dictum. Sed sit amet dolor a neque dictum malesuada et nec lectus. Aenean tristique ornare nisl, at imperdiet magna placerat eget. Suspendisse ut blandit elit, sit amet iaculis ex. Nam vel varius velit. Aenean pretium scelerisque enim, quis mollis nunc finibus eu.
Headline 3
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Phasellus ac enim id metus rutrum blandit sed non dolor. Pellentesque feugiat odio eu imperdiet rutrum. Duis consectetur porttitor enim, id elementum nibh tempus in. Nulla ut massa rutrum, ullamcorper dui et, posuere velit. Cras viverra, tortor at porta pulvinar, libero eros pulvinar orci, sed pulvinar neque felis eget dui. Fusce laoreet libero vitae nunc hendrerit consequat. Nunc eget bibendum turpis. Vestibulum pulvinar interdum mauris nec congue. Etiam id nunc ac risus dictum semper sed in nisl.
Cras ultrices eu massa vitae congue. Vestibulum arcu enim, congue id lacus at, laoreet fringilla tortor. Praesent libero nunc, maximus vel suscipit nec, fringilla sed augue. Praesent porttitor vehicula efficitur. Aenean ac bibendum lectus. Praesent vestibulum velit ut nunc accumsan, non molestie erat pretium. In a ante vulputate, semper sapien at, tristique erat. Morbi vitae euismod eros, at pulvinar erat. Vivamus auctor arcu sed tellus egestas, vitae imperdiet eros viverra. Nulla molestie sapien vitae ipsum pulvinar dictum. Sed sit amet dolor a neque dictum malesuada et nec lectus. Aenean tristique ornare nisl, at imperdiet magna placerat eget. Suspendisse ut blandit elit, sit amet iaculis ex. Nam vel varius velit. Aenean pretium scelerisque enim, quis mollis nunc finibus eu.