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Revision as of 04:42, 19 October 2021
We have added 3 new basic parts and 4 composite parts to the registry with information of its sequences and some details from the literature. These parts come from key modules of our biosensor constructs such as the transcriptional amplifiers, intracellular arsR control systems and the violet reporter.
I. Competence induction and transformation:
| Part’s link | Type | Part’s name | Short description |
|---|---|---|---|
| BBa_K4007000 | Basic | mRFP1-Violet | Coding sequence of a violet mRFP1 variant. |
| BBa_K4007001 | Basic | pHrpL (σ54 optimized) | Sequence of inducible promoter pHrpL optimized by Wang et al. adopting the consensus σ54 promoter sequence for increased output expression. |
| BBa_K4007002 | Basic | RinA_p80α protein | Coding sequence of a proteín that acts as activator for the transcription in an ultrasensitive way. |
| BBa_K4007004 | Composite | Ultrasensitive biosensing complex por arsenic (#1) | Genetic construct with a double amplifying cascade that offers sub-5 ppb [As] sensitivities with an coloured output. |
| BBa_K4007005 | Composite | Ultrasensitive biosensing complex por arsenic (#2) | Genetic construct with a simple amplifying system that offers sub-10 ppb [As] sensitivities with an coloured output. |
| BBa_K4007007 | Composite | Intracellular arsR density control system (medium density) | Genetic construct with a medium strength constitutive promoter controlling the arsR gene expression. |
| BBa_K4007008 | Composite | Intracellular arsR density control system (high density) | Genetic construct with a high strength constitutive promoter controlling the arsR gene expression. |
Synthetic biology laboratory manual:
At the beginning of the competition some team members encountered difficulties with the accessibility of reliable information, especially in our native language. For this reason we found that it was a very good idea to research, compile, translate and materialize several of the most used techniques in synthetic biology laboratories into a comprehensive and easy to understand laboratory manual. It was very helpful to understand many of the basics of the experiments that we were planning to carry on and we hope it will also be of great help to other Spanish-speaking teams.
Device manual:
We elaborated a very detailed manual for our device which includes instructions for assembly and use, 3D models, exact dimensions for its parts and some downloadable files for 3D printing. We hope it will also be of great help to other future projects with similar hardware in mind.