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| − | <!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Parts | iGEM Michigan</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:Michigan/css/contentCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:Michigan"><span>iGEM </span>Michigan</a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Team">Team</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Collaborations">Collaborations</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Contribution">Contribution</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Modeling">Modeling</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Engineering">Engineering</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Notebook">Notebook</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Results">Results</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Implementation">Implementation</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarPartsDropdown" role="button">Parts</a><div aria-labelledby="navbarPartsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Parts">Parts</a></div></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:Michigan/Human_Practices">Human Practices</a></li></ul></div><div class="d-flex" id="themeSwitchWrapper"><i class="far fa-sun"></i><div id="themeSwitch"><label class="switch" for="themeSwitchInput"><input id="themeSwitchInput" type="checkbox"/><span class="slider round"></span></label></div><i class="far fa-moon"></i></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Parts</h1><p class="lead pl-1"></p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><p>The following parts were used or developed by MSBT over the course of the project.</p><table><thead><tr><th>Part Number</th><th>Name</th><th>Description</th></tr></thead><tbody><tr><td>BBa_K4037000</td><td>Encapsulin+mNeonGreen+HisTag+SpyTag+AlphaFactor</td><td>Plasmid that contains sequence for a T4 <em>T. Maritima</em> encapsulin and mNeonGreen fluorescent cargo co-assembled inside of it.</td></tr><tr><td>BBa_K1159201</td><td>Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]</td><td>Spytag oligopeptide sequence that is recognized by SpyCatcher protein</td></tr><tr><td>BBa_K1223006</td><td>His-Tag</td><td>C-terminal His tag for protein purification</td></tr><tr><td>BBa_K4037001</td><td>Primer A for inverse fusion PCR</td><td>Primer A used for alpha factor addition</td></tr><tr><td>BBa_K4037002</td><td>Primer B for inverse fusion PCR</td><td>Primer B used for alpha factor addition</td></tr><tr><td>BBa_K4037003</td><td>Primer C for inverse fusion PCR</td><td>Primer C used for alpha factor addition</td></tr><tr><td>BBa_K4037004</td><td>Fragment Forward Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr><tr><td>BBa_K4037005</td><td>Vector Reverse Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr><tr><td>BBa_K4037006</td><td>Vector Forward Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr><tr><td> BBa_K4037006</td><td>Fragment Reverse Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr></tbody></table></article></div></div></div></main><footer><div class="container"><p>Built using the iGEM Wiki Starter Pack by BITS Goa.</p><p>Code released under the MIT license.</p><p>Based on <a href="https://getbootstrap.com">Bootstrap</a> and themes <a href="https://bootswatch.com/flatly/">Flatly</a> and <a href="https://bootswatch.com/darkly/">Darkly</a> from <a href="https://bootswatch.com/">Bootswatch</a>.</p><p>Icons from <a href="flaticon.com">Flaticon</a>. Images from <a href="https://unsplash.com">Unsplash</a>. Web fonts from <a href="https://fonts.google.com">Google</a>.</p></div></footer><script src="https://2021.igem.org/Template:Michigan/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html> | + | <!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Parts | iGEM Michigan</title><script src="https://2020.igem.org/common/MathJax-2.5-latest/MathJax.js?config=TeX-AMS-MML_HTMLorMML"></script><link href="https://2021.igem.org/Template:Michigan/css/contentCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:Michigan"><span>iGEM </span>Michigan</a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul class="navbar-nav ml-auto"><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarTeamDropdown" role="button">Team</a><div aria-labelledby="navbarTeamDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Team">Team</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Attributions">Attributions</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Collaborations">Collaborations</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarProjectDropdown" role="button">Project</a><div aria-labelledby="navbarProjectDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Contribution">Contribution</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Description">Description</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Modeling">Modeling</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Experiments">Experiments</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Engineering">Engineering</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Notebook">Notebook</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Results">Results</a><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Implementation">Implementation</a></div></li><li class="nav-item dropdown"><a aria-expanded="false" aria-haspopup="true" class="nav-link dropdown-toggle" data-toggle="dropdown" href="#" id="navbarPartsDropdown" role="button">Parts</a><div aria-labelledby="navbarPartsDropdown" class="dropdown-menu"><a class="dropdown-item" href="https://2021.igem.org/Team:Michigan/Parts">Parts</a></div></li><li class="nav-item"><a class="nav-link" href="https://2021.igem.org/Team:Michigan/Human_Practices">Human Practices</a></li></ul></div><div class="d-flex" id="themeSwitchWrapper"><i class="far fa-sun"></i><div id="themeSwitch"><label class="switch" for="themeSwitchInput"><input id="themeSwitchInput" type="checkbox"/><span class="slider round"></span></label></div><i class="far fa-moon"></i></div></div></nav><header class="d-flex justify-content-center align-items-center"><div class="container"><h1>Parts</h1><p class="lead pl-1"></p><hr class="my-4"/></div></header><main><div class="container"><div class="row"><div class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><p>The following parts were used or developed by MSBT over the course of the project.</p><table><thead><tr><th>Part Number</th><th>Name</th><th>Description</th></tr></thead><tbody><tr><td>BBa_K4037000</td><td>Encapsulin+mNeonGreen+HisTag+SpyTag+AlphaFactor</td><td>Plasmid that contains sequence for a T4 <em>T. Maritima</em> encapsulin and mNeonGreen fluorescent cargo co-assembled inside of it.</td></tr><tr><td>BBa_K1159201</td><td>Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]</td><td>Spytag oligopeptide sequence that is recognized by SpyCatcher protein</td></tr><tr><td>BBa_K1223006</td><td>His-Tag</td><td>C-terminal His tag for protein purification</td></tr><tr><td>BBa_K4037001</td><td>Primer A for inverse fusion PCR</td><td>Primer A used for alpha factor addition</td></tr><tr><td>BBa_K4037002</td><td>Primer B for inverse fusion PCR</td><td>Primer B used for alpha factor addition</td></tr><tr><td>BBa_K4037003</td><td>Primer C for inverse fusion PCR</td><td>Primer C used for alpha factor addition</td></tr><tr><td>BBa_K4037004</td><td>Fragment Forward Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr><tr><td>BBa_K4037005</td><td>Vector Reverse Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr><tr><td>BBa_K4037006</td><td>Vector Forward Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr><tr><td> BBa_K4037007</td><td>Fragment Reverse Gibson Assembly primer</td><td>Primer used for alpha factor addition through Gibson Assembly</td></tr></tbody></table></article></div></div></div></main><footer><div class="container"><p>Built using the iGEM Wiki Starter Pack by BITS Goa.</p><p>Code released under the MIT license.</p><p>Based on <a href="https://getbootstrap.com">Bootstrap</a> and themes <a href="https://bootswatch.com/flatly/">Flatly</a> and <a href="https://bootswatch.com/darkly/">Darkly</a> from <a href="https://bootswatch.com/">Bootswatch</a>.</p><p>Icons from <a href="flaticon.com">Flaticon</a>. Images from <a href="https://unsplash.com">Unsplash</a>. Web fonts from <a href="https://fonts.google.com">Google</a>.</p></div></footer><script src="https://2021.igem.org/Template:Michigan/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html> |
