Difference between revisions of "Team:CPU CHINA/Collaborations"

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         <h1 id="title">COLLABORATION</h1>
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         <h1 id="title">EDUCATION AND COMMUNICATION</h1>
 
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             <nav>
 
             <nav>
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                 <div class="catalogue-title">COLLABORATION</div>
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                 <div class="catalogue-title">EDUCATION AND COMMUNICATION</div>
 
                 <ul class="catalogue">
 
                 <ul class="catalogue">
 
                     <li class="num">
 
                     <li class="num">
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                         <a href="#section1" title="CONFERENCES AND MEETUPS">CONFERENCES AND MEETUPS</a>
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                         <a href="#section1" title="INTRODUCTION">INTRODUCTION</a>
 
                     </li>
 
                     </li>
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                     <li class="num">
 
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                         <a href="#section2" title="TEAM COLLABORATIONS">TEAM COLLABORATIONS</a>
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                         <a href="#section2" title="ICII: SPONSOR OF JOINT EDUCATION ACTIVITIES">ICII: SPONSOR OF JOINT
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                            EDUCATION ACTIVITIES</a>
 
                     </li>
 
                     </li>
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                     <li class="num">
 
                     <li class="num">
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                         <a href="#section3" title="SPECIAL ISSUE: INTO CHINA, INTO iGEM (ICII)">SPECIAL ISSUE: INTO
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                         <a href="#section3" title="APPROACHING SYNTHETIC BIOLOGY">APPROACHING SYNTHETIC BIOLOGY</a>
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                             CHINA, INTO iGEM (ICII)</a>
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                    </li>
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                    <li class="num">
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                        <a href="#section4" title="PROMOTING GARBAGE CLASSIFICATION">PROMOTING GARBAGE
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                            CLASSIFICATION</a>
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                    </li>
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                    <li class="num">
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                        <a href="#section5"
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                            title="MEDDY&#xFF1A;RELIGHT MY STAR PUBLIC ART EXHIBITION"><em>MEDDY&#xFF1A;RELIGHT MY
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                                STAR</em> <em>PUBLIC</em> ART EXHIBITION</a>
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                    </li>
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                    <li class="num">
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                        <a href="#section6" title="MEDDY IN WONDERLAND--COMIC STRIP">MEDDY IN WONDERLAND--COMIC
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                             STRIP</a>
 
                     </li>
 
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             <div class="section" id="section1">
 
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                 <h2>CONFERENCES AND MEETUPS</h2>
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                 <h2 class="mume-header" id="introduction">INTRODUCTION</h2>
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                <h3>THE CONFERENCE OF NANJING iGEM TEAMS</h3>
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                <p><span>On May 5th, 2021, the conference of Nanjing iGEM teams hosted by NJU-China was held in the
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                        Nanjing
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                        University. We were invited to participate in this very collaborative event in which we were
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                        able to
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                        acquire actual feedback of project design from others. At the meeting, each team made a brief
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                        introduction to their projects and future plans. Nanjing iGEM Association, a regional
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                        cooperation
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                        and exchange organization for iGEM teams in Nanjing, was officially established after the
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                        meeting.</span></p>
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                <img src="https://static.igem.org/mediawiki/2021/4/4b/T--CPU_CHINA--Collaboration-1.png"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-1">
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                <p class="imgdescribe"><strong><span>Fig. 1 Members of CPU_CHINA presenting project
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                            background</span></strong></p>
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                <img src="https://static.igem.org/mediawiki/2021/f/f4/T--CPU_CHINA--Collaboration-2.png"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-2">
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                <p class="imgdescribe"><strong><span>Fig. 2 Group photo of CPU_CHINA participants during the conference
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                            of Nanjing iGEM
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                            teams</span></strong></p>
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                <img src="https://static.igem.org/mediawiki/2021/4/43/T--CPU_CHINA--Collaboration-3.png"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-3">
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                <p class="imgdescribe"><strong><span>Fig. 3 Group photo of all the participants of the conference of
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                            Nanjing iGEM
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                            teams.</span></strong></p>
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                <p><span>We gained suggestions that we shall further determine the authenticity of H2O2-producing
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                        hypothesis
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                        of laccase before we decided to implement it into experiments. In addition, we had received
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                        several
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                        new cooperation intentions with other iGEM teams. As a result, we planned to team up with
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                        NAU-China
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                        for future cooperations. This led to the launch of an educational event, i.e. &quot;Into China,
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                        Into
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                        iGEM&quot;. We will put more explanation about this event later in this page.</span></p>
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                <h3>YEAST MEETUP</h3>
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                <p><span>As we selected </span><em><span>Pichia pastoris</span></em><span> as our engineered chassis, it
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                        is
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                        of vital importance that we acquire further insights of how to utilize this organism correctly
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                        and
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                        properly. Therefore, on August 22nd, 2021, we participated in the virtual Yeast Meetup hosted by
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                        UESTC-China. We shared our experience and specific details about culturing and fermenting
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                    </span><em><span>Pichia pastoris.</span></em></p>
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                <img src="https://static.igem.org/mediawiki/2021/d/d5/T--CPU_CHINA--Collaboration-4.png"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-4">
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                <p class="imgdescribe"><strong><span>Fig. 4 Participants of the virtual Yeast Meetup.</span></strong>
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                </p>
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                <p><span>As the host of this meetup, UESTC-China shared their encyclopedical knowledge about summarized
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                        tips
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                        and cautions for using </span><em><span>P.pastoris</span></em><span> as the engineered chassis.
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                        In
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                        particular, their share of detecting miscellaneous bacterial infection in cultures of
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                    </span><em><span>P.pastoris</span></em><span> with centrifugation reminded us of an abnormal
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                        phenomenon
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                        we discovered when preparing </span><em><span>P.pastoris</span></em><span> competent cell, as
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                        the
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                        contaminated </span><em><span>P.pastoris</span></em><span> culture was not able to condense
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                        tightly
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                        at the bottom of centrifuge tube after centrifuging at 4000 rpm for 10 min. Using this abnormal
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                        competent cell for electrotransformation may lead to the failure of acquiring recombinant single
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                        cell colonies.</span></p>
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                <p><span>In this meetup, we found that a lot of teams were puzzled on how to perform yeast colony PCR
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                        with a
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                        higher success rate. They tried many kinds of yeast colony pretreatments such as adding lyticase
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                        or
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                        fast freezing-thawing, but with unstable success rate. Therefore, we provided our yeast colony
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                        PCR
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                        protocol of using 1 μl diluted colony sample (simply picks a portion of one single colony and
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                        dilutes the cells with 10μl ddH</span><sub><span>2</span></sub><span>O) as our template source,
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                        then
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                        setting a pre-denaturation stage at 98℃ for 10 min before switching to the normal colony PCR
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                        program.</span></p>
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                <img src="https://static.igem.org/mediawiki/2021/c/c0/T--CPU_CHINA--Collaboration-5.png"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-5">
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                <p class="imgdescribe"><strong><span>Fig. 5 One of our Powerpoint slides showing protocols of plasmid
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                            linerization and
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                            pretreatment before electrotransformation.</span></strong></p>
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                <p><span>As a result, we benefited a lot from discussing various problems we encountered when dealing
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                        with
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                        this organism. We also got the opportunity of sharing many unexpected &quot;surprises&quot; we
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                        experienced during culturing </span><em><span>P. pastoris</span></em><span>. In addition, we
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                        established a further cooperation intention with team UESTC-China.</span></p>
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                <h3>THE 8th CCiC (CONFERENCE OF CHINA iGEMer COMMUNITY)</h3>
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                <p><span>The Conference of China iGEMer Community (CCiC) is the biggest annual event for iGEMers from
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                        different teams around China to gather together, where participants are able to display their
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                        project, to exchange ideas and suggestions, as well as to look for of partners that share
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                        similar
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                        aspirations. In 2021, despite difficulties caused by the COVID-19 pandemic, we still made our
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                        way to
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                        the 8th CCiC hosted in Shanghai from August 27 to 29. </span></p>
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                <p><span>The conference had invited several honored guests ranging from prestigious academicians to
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                        successful entrepreneurs to deliver presentations regarding understanding of the developmental
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                        prospects of synthetic biology, personal experiences and field insights, and certain cautions of
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                        conducting an iGEM project. Also, during poster sessions, we made visits to other teams.
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                        Particularly, members from DUT_China welcomed us warmly. Similar in project goal, we had
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                        constructive conversations with each other and further determined our partner
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                        relationship.</span>
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                </p>
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                <img src="https://static.igem.org/mediawiki/2021/8/8a/T--CPU_CHINA--Collaboration--6.jpg"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration--6">
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                <p class="imgdescribe"><strong><span>Fig. 6 The photograph of team members from DUT_China and CPU_CHINA
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                            at CCiC8
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                            venue.</span></strong></p>
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                <p><span>We made a brief report of the design and progress of our project. Two judges informed us with
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                        vital
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                        feedbacks. What&#39;s more, during the period of online Q&amp;A session, Huandi Xu, the
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                        president of
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                        CCiC executive committee, evaluated our project and provided us with helpful advice on improving
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                        presentation effectiveness. In respond to all these feedbacks, firstly, we need to better
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                        specify
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                        the application scenarios of our project, so that we can further modify our product with
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                        pertinence;
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                        secondly, we shall focus on proving or demonstrating that our protein-nucleic acid complex could
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                        converge the advantages of the three PE-degrading parts to exert a more significant functional
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                        improvement when compared with independently applying the parts into use.</span></p>
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                <p><span>At CCiC8, we stood out from the crowd of teams and entered the finalist of Best Presentation
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                        Awards. </span></p>
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                <img src="https://static.igem.org/mediawiki/2021/5/5c/T--CPU_CHINA--Collaboration--7.jpg"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration--7">
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                <p class="imgdescribe"><strong><span>Fig. 7 The &quot;Best Presentation Finalist&quot;
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                            medal</span></strong></p>
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                <p>&nbsp;</p>
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                <h3>CRISPR/Cas COMMUNICATION MEETING</h3>
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                <p><span>Since CRISPR-related techniques were included in our project, on September 12th, we were
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                        invited to
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                        attend the virtual CRISPR/Cas Communication Meeting hosted by team Tianjin.</span></p>
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                <img src="https://static.igem.org/mediawiki/2021/5/5d/T--CPU_CHINA--Collaboration-8.png"
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                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-CC大会">
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                <p class="imgdescribe"><strong><span>Fig. 8 Ongoing scene of CRISPR/Cas communication
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                            meeting</span></strong></p>
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                <p><span>During the meeting, we had an in-depth exchange with teams using the CRISPR/Cas9 system,
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                        discussed
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                        and resolved the problems that each team encountered. We received assistance and advice from
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                        team
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                        Tianjin &amp; NEFU_China, who suggested us to perform SDS-PAGE of lower separation gel
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                        concentrations to characterize our SpyCatcher/SpyTag linkage system. The separation of our
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                        multiplex
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                        protein system was significantly improved after we changed the conditions. What&#39;s more, they
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                        also suggested that separations could be performed by adapting pull-down experiments using an
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                        altered label.</span></p>
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                <p><span>After the meeting, we worked together on the writing of</span></p>
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                <div id="downLoad">
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                    <span>CRISPR/Cas APPLICATION
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                        GUIDELINES.pdf</span>
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                    <a href="https://static.igem.org/mediawiki/2021/8/86/T--Tianjin--Crispr-application.pdf"
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                        download>ACCESS</a>
+
−
                </div>
+
−
                <h3>ROUND TABLE CLUB: PLASTICASE</h3>
+
−
                <p><span>In late spring and early summer, team CPU_CHINA, as the organizer, united many iGEM teams to
+
−
                        establish Plasticase--a community dedicated in solving the problem of plastic pollution using
+
−
                        approaches derived from synthetic biology. From May to September, members of Plasticase shared
+
−
                        cutting-edge information, looked for potential partners, and put forward solutions regarding
+
−
                        various
+
−
                        problems. </span></p>
+
−
                <img src="https://static.igem.org/mediawiki/2021/2/23/T--CPU_CHINA--Collaboration-9.png"
+
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-9">
+
−
                <p class="imgdescribe"><strong><span>Fig. 9 Teams consist of Plasticase Confederation</span></strong>
+
−
                </p>
+
−
                <p><strong><a href="https://2021.igem.org/Team:CPU_CHINA/Plasticase">Plasticase</a></strong></p>
+
−
                <p><span>In order to have a deeper understanding between every team, we decided to launch a round table
+
−
                        club
+
−
                        which allowed teams to exchange projects and explore opportunities for in-depth
+
−
                        cooperation.</span>
+
−
                </p>
+
−
                <img src="https://static.igem.org/mediawiki/2021/e/ec/T--CPU_CHINA--Collaboration-10.png"
+
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-10">
+
−
                <p class="imgdescribe"><strong><span>Fig. 10 Participants of Round Table Club</span></strong></p>
+
−
                <p><span>With the help of IvyMaker-China, we invited Dr. Huiming Xiang from Shanghai Environment Group
+
−
                        Company Limited to introduce the migration and hazards of microplastics in the environment to
+
−
                        us.
+
−
                        Later, iGEMers from BJEA_China, BUCT-China, CPU_CHINA, DUT_China, IvyMaker-China, KEYSTONE,
+
−
                        SMS_Shenzhen and TJUSLS_China introduced their respective projects. As judges, Prof. Meiling Lu
+
−
                        and
+
−
                        Prof. Meirong Chen from China Pharmaceutical University also put forward their own suggestions
+
−
                        and
+
−
                        opinions. Whether it is a member of wet lab or human practices, everyone had learned a
+
−
                        lot.</span>
+
−
                </p>
+
−
                <p><span>The round table club had brought every member of Plasticase closer with each other. We believe
+
−
                        that
+
−
                        the deeper sense of belonging to Plasticase in every member would facilitate further discussions
+
−
                        regarding progresses in the field of synthetic biology and plastic degradation during and after
+
−
                        the
+
−
                        iGEM competition.</span></p>
+
−
                <p><span>For further details of Plasticase Confederation, see <strong><a href="https://2021.igem.org/Team:CPU_CHINA/Plasticase">Plasticase</a></strong></span><em><span></span></em><span>.</span></p>
+
  
 +
                <p>As an iGEMer, it is our responsibility to make synthetic biology more accessible and attractive. This
 +
                    year, our team recognized the limitations of traditional publicity activities which were restricted
 +
                    in small scopes or targeted on specialists who had already equipped with synthetic biology
 +
                    knowledge. Thus, after elaborate consideration, we launched ICII, a large-scale national publicity
 +
                    campaign, carrying out different science popularization and education activities for classified
 +
                    audience according to their age and level of education. CPU_CHINA also combined synthetic biology
 +
                    with other fields, further launched art exhibitions and produced comic strips. And for better giving
 +
                    publicity to synthetic biology and publishing popular science articles, we have created team
 +
                    accounts in bilibili, MicroBlog and other major social platforms, actively adapted to the wave of
 +
                    new media development.</p>
 
             </div>
 
             </div>
 
             <div class="section" id="section2">
 
             <div class="section" id="section2">
−
                 <h2>TEAM COLLABORATIONS</h2>
+
                 <h2 class="mume-header" id="icii-sponsor-of-joint-education-activities">ICII: SPONSOR OF JOINT EDUCATION
−
                <h3>DUT_China</h3>
+
                    ACTIVITIES</h2>
−
                 <p><span>Team DUT_China shared similar goals of attenuating plastic pollution with us. They contacted us
+
 
−
                        after watching the playback of our project introduction at the conference of Nanjing iGEM teams.
+
                 <p>ICII, namely &quot;Into CHINA, Into iGEM&quot;, is a summer tour activity to publicize synthetic
−
                        They invited us to attend Laccase Meeting held by team TU_Kaiserslautern 2020 on May 18th, where
+
                    biology and iGEM, jointly sponsored by CPU_CHINA and NAU-CHINA. As an Education&amp;Collaboration
−
                        we
+
                    activity hosted by two teams, 18 iGEM teams from all over the country were invited. The teams showed
−
                        discussed the aforementioned H2O2-producing hypothesis of laccase with team members of
+
                    the project information and posters of all the cooperating teams when they preached in their
−
                        TU_Kaiserslautern.</span></p>
+
                    locations, and shot vlogs to record the interesting process of preaching and urban culture.</p>
−
                 <img src="https://static.igem.org/mediawiki/2021/e/ec/T--CPU_CHINA--Collaboration-11.png"
+
                <p><img src alt></p>
−
                     referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-10">
+
                 <img src="https://static.igem.org/mediawiki/2021/e/e5/T--CPU_CHINA--Education_%26_Communication--Figure_1.jpg"
−
                 <p class="imgdescribe"><strong><span>Fig. 11 Participants of Laccase Meeting.</span></strong></p>
+
                     alt="">
−
                 <p><span>After the Laccase Meeting, several online meetups between the two teams were held around June
+
                 <p class="imgdescribe"><strong>Fig. 1 Poster of Into CHINA, Into iGEM</strong></p>
−
                        for
+
                 <p>With the collaboration of multiple teams throughout the country, the impact of synthetic biology is
−
                        introducing respective projects, further discussing and exchanging details, and seeking
+
                    hoped to be wider, broader and more profound. By approaching more cities and larger scale audiences,
−
                        opportunities of partnership. We met with each other at the 8th CCiC as described above. </span>
+
                    presenting new technology and new ideas in more specific forms, we aimed that our operation would
 +
                    achieve the common aspiration of projecting science popularization and iGEM event publicity, making
 +
                    synthetic biology more acceptable and impressive.</p>
 +
                <p>The sponsors compiled the vlogs of all the teams, and combined the presentation process and urban
 +
                    cultural publicity to edit a summary video. We hope to unfolded the culture of different cities in
 +
                    China to the iGEM world through the final video, showing the scientific and technological strength
 +
                    of China and the enthusiasm of the Chinese people for the development of biotechnology. Meanwhile,
 +
                    ICII could act as an entry point for the world to have a more comprehensive understanding of China.
 
                 </p>
 
                 </p>
−
                 <img src="https://static.igem.org/mediawiki/2021/e/ef/T--CPU_CHINA--Collaboration-12.png"
+
                 <p><img src alt></p>
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-11">
+
                <video controls style="width: 100%;">
−
                <p class="imgdescribe"><strong><span>Fig. 12 Regular inter-team meetups between DUT_China and
+
                    <source src="https://static.igem.org/mediawiki/2021/c/ca/T--CPU_CHINA--ICII.mp4">
−
                             CPU_CHINA</span></strong></p>
+
                        <p>Your browser doesn't support HTML5 video. Here is
−
                <p><span>Apart from the communications and assistances described above, DUT_China also provided valuable
+
                             a <a href="https://static.igem.org/mediawiki/2021/c/ca/T--CPU_CHINA--ICII.mp4">link to the
−
                        opportunities for us to reach out to experts in industrial community. They as well obtained
+
                                video</a> instead.</p>
−
                        inspirations from our project designs. (For more information on how the two teams help with each
+
                </video>
−
                        other, see </span><em><span>Partnership page</span></em><span>)</span></p>
+
                 <p class="imgdescribe">Video 1. Vlog of Into CHINA, Into iGEM</p>
−
                <h3>NAU-CHINA</h3>
+
                 <p>These lectures are like a large-scale sowing, laying the seed of using synthetic biology to benefit
−
                <p><span>We met team NAU-CHINA at the conference of Nanjing iGEM teams in May. They not only put forward
+
                    society and change the world for children and adolescents. We are looking forward to their
−
                        vital advice for our project during the conference, but also showed the strongest willingness to
+
                    germination and growth tomorrow.</p>
−
                        collaborate with us after we met with each other. </span></p>
+
                 <p><strong>For more cooperation details, click the button below to view another separate page.</strong>
−
                <p><span>Soon, on June 15th, the first online meeting was hosted between Human Practice members of the
+
−
                        two
+
−
                        teams. </span></p>
+
−
                <img src="https://static.igem.org/mediawiki/2021/b/b9/T--CPU_CHINA--Collaboration-13.png"
+
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-12">
+
−
                <p class="imgdescribe"><strong><span>Fig. 13 The Human Practices members of the two teams discussing
+
−
                            formats of education
+
−
                            activities.</span></strong></p>
+
−
                 <p><span>We discussed ways of carrying out our educational activities and realized that uniting teams
+
−
                        from
+
−
                        all over the nation to join us may be a good idea. Therefore, with the aim of maximizing our
+
−
                        collaborative partners, we sent invitational emails to participating teams located in different
+
−
                        provinces of China. Eventually, a total of 17 iGEM teams were recruited. Furthermore, we
+
−
                        determined
+
−
                        the format and workflow of this very activity, namely &quot;ICII&quot; (For further reading of
+
−
                        ICII,
+
−
                        please check the description below, as well as our </span><em><span>Education
+
−
                            page</span></em><span>). We decided to demonstrate our achievements by displaying briefings
+
−
                        on a
+
−
                        map, as well as making vlog clips, so that they would be more intuitive and vivid for people to
+
−
                        understand.</span></p>
+
−
                 <p><span>In the following periods, we allocated our respective responsibilities, finished the first
+
−
                        promotion article, and prompting preaching activities hosted by participating teams around the
+
−
                        nation. In addition, scriptwriting and video clipping of different materials from various teams
+
−
                        were
+
−
                        also important jobs that we accomplished.</span></p>
+
−
                <p>&nbsp;</p>
+
−
                <h3>NEFU_China</h3>
+
−
                <p><span>Team NEFU_China contacted us in August hoping to be more familiar with the role and function of
+
−
                        CRISPR/dCas9 system in our project design. Therefore, they hosted a short meeting and shared the
+
−
                        project design with each other. We also provided several experimental suggestions on how to
+
−
                        verify
+
−
                        the assembly of dCas9 with sgRNAs and dsDNA.</span></p>
+
−
                <img src="https://static.igem.org/mediawiki/2021/1/1a/T--CPU_CHINA--Collaboration-14.png"
+
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-13">
+
−
                 <p class="imgdescribe"><strong><span>Fig. 14 Participants of the short meeting between CPU_CHINA and
+
−
                            NEFU_CHINA.</span></strong>
+
 
                 </p>
 
                 </p>
−
                 <p><span>Later, during the online Q&amp;A session of CCiC, NEFU_CHINA consulted us about difficulties
+
                 <p>(<a href="https://2021.igem.org/Team:CPU_CHINA/Collaborations">Team:CPU CHINA/Collaborations -
−
                        they
+
                         2021.igem.org</a>)</p>
−
                        met of assembling their system </span><em><span>in vivo**.</span></em><span> We thought that
+
−
                        their
+
−
                        system was too large and sophisticated to be assembled in bacteria, so we suggested that they
+
−
                        should
+
−
                        tackle this problem by trying to verify its assembly </span><em><span>in
+
−
                            vitro</span></em><span>, as
+
−
                        we did in our project. </span></p>
+
−
                <p><span>We were glad to hear from members of NEFU_CHINA on September 5th. They informed us that
+
−
                        they
+
−
                        listened to our advice and had successfully obtained components of their system
+
−
                    </span><em><span>in
+
−
                            vitro</span></em><span>, and they would try to assemble it </span><em><span>in
+
−
                            vitro</span></em><span>. </span></p>
+
−
                <p>&nbsp;</p>
+
−
                <h3>Tianjin</h3>
+
−
                <p><span>Team Tianjin reached out to us after we mentioned the usage of CRISPR/dCas9 techniques in
+
−
                        our
+
−
                        project during the presentation stage of CCiC. We were subsequently invited to participate
+
−
                        in the
+
−
                        aforementioned CRISPR/Cas meeting they hosted, where several teams applying this technique
+
−
                        gathered
+
−
                        together to exchange experiences and share experiments.</span></p>
+
−
                <p><span>During the meeting, the two teams exchanged academic suggestions with each other, and tried
+
−
                        to work
+
−
                        out solutions on several problems we encountered in our experiments. We also discussed
+
−
                        difficulties
+
−
                        and solutions with other participating teams. After the conference, we accomplished the
+
−
                        CRISPER/Cas
+
−
                        handbook, hoping to give experienced references for future teams.</span></p>
+
−
                <p><span>After the meeting, the two teams continued to collaborate with each other throughout the
+
−
                        rest of
+
−
                        iGEM 2021 season (For more information on how the two teams help with each other, see
+
−
                    </span><em><span>Partnership page</span></em><span>). </span></p>
+
−
                <img src="https://static.igem.org/mediawiki/2021/d/d2/T--CPU_CHINA--Collaboration-15.png"
+
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-TJU Meeting">
+
−
                <p class="imgdescribe"><strong><span>Fig. 15 Members of the two teams discussing issues about
+
−
                            experiment
+
−
                            progress.</span></strong></p>
+
−
                <h3>Tongji_China</h3>
+
−
                <p><span>Team Tongji_China is the first team that we made communication with. Early on March 28th,
+
−
                        with the
+
−
                        help of the instructors from both teams, we met online to discuss literally everything about
+
−
                        iGEM,
+
−
                        from familiarizing ourselves with the competition calendar to brainstorming with each other
+
−
                        for
+
−
                        threads of project design. </span></p>
+
−
                <img src="https://static.igem.org/mediawiki/2021/f/fa/T--CPU_CHINA--Collaboration-16.png"
+
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-Tongji-1">
+
−
                <p class="imgdescribe"><strong><span>Fig. 16 The first ever meetup of team CPU_CHINA with
+
−
                            Tongji_China</span></strong></p>
+
−
                <p><span>At the initial meeting, the two teams determined that we shall both work on environmental
+
−
                         projects
+
−
                        this year, thus potential collaborations regarding projects was possible.</span></p>
+
−
                <p><span>Later in May, members of Tongji_China made a visit to the campus of CPU_CHINA. Together,
+
−
                        the two
+
−
                        teams made further discussions on updates of respective project design and human practices
+
−
                        plans. We
+
−
                        learned that Tongji_China dedicated to reduce stinks produced by rotten food in kitchen
+
−
                        garbage
+
−
                        dustbins, thereby improving air quality. After the meeting, we showed our guests around our
+
−
                        laboratory and different places in the campus.</span></p>
+
−
                <img src="https://static.igem.org/mediawiki/2021/4/48/T--CPU_CHINA--Collaboration-17.png"
+
−
                    referrerpolicy="no-referrer" alt="T--CPU_CHINA--Collaboration-Tongji-2">
+
−
                <p class="imgdescribe"><strong><span>Fig.17 Members of the Tongji_China introducing project
+
−
                            background
+
−
                            to team
+
−
                            CPU_CHINA.</span></strong></p>
+
−
                <p><span>Since the two teams shared a similar focus on environmental issues, as we were conducting
+
−
                        survey in
+
−
                        the waste transfer station of Jiangning High-tech Zone, we interviewed staffs about current
+
−
                        treatments for garbage stinks, and discussed the possibility of applying engineered strains
+
−
                        to solve
+
−
                        this problem. After that, we provided the related feedback to Tongji_China.</span></p>
+
−
                <p>&nbsp;</p>
+
−
                <h3>UESTC-China</h3>
+
−
                <p><span>We met team UESTC-China through China iGEMer Online Meetup back in May. After discovering
+
−
                        that both
+
−
                        teams used Pichia pastoris strain GS115 to express target protein, we engaged in further
+
−
                        communication and collaboration, such as discussing the problems encountered in our
+
−
                        experiments and
+
−
                        carried out educational work in Human Practices.</span></p>
+
−
                <p><span>We both encountered many problems when using GS115 to express our protein, such as
+
−
                        contamination
+
−
                        issues caused by unknown species of miscellaneous bacteria, plasmid DNA cross-pollution in
+
−
                        yeast
+
−
                        electroporation issues, as well as numerous puzzling observations caused by the mysterious
+
−
                        expression profile of </span><em><span>Pichia pastoris</span></em><span>. Our common daily
+
−
                        complaints towards this very chassis had brought the two teams closer together, facilitating
+
−
                        lots of
+
−
                        conversations and helping hands, and eventually, partnership. (For more information on how
+
−
                        the two
+
−
                        teams help with each other, see </span><em><span>Partnership page</span></em><span>)</span>
+
−
                </p>
+
−
                <p><span>In addition to assistances of experiment-related issues, we also communicated with each
+
−
                        other
+
−
                        regarding the filling of Safety Form, as well as participating in the virtual Yeast Meetup
+
−
                        described
+
−
                        above.</span></p>
+
 
             </div>
 
             </div>
 
             <div class="section" id="section3">
 
             <div class="section" id="section3">
−
                 <h2>SPECIAL ISSUE: INTO CHINA, INTO iGEM (ICII)</h2>
+
                 <h2 class="mume-header" id="approaching-synthetic-biology">APPROACHING SYNTHETIC BIOLOGY</h2>
−
                 <a href="https://2021.igem.org/Team:CPU_CHINA/Map"><img src="https://static.igem.org/mediawiki/2021/1/16/T--CPU_CHINA--Collaboration-19.png"></a>
+
 
−
                <p class="imgdescribe"><strong><span>Fig. 18 The participating teams are located in different regions of
+
                 <h3 class="mume-header" id="primary-school-wonderful-microbial-factory">Primary school: Wonderful
−
                            mainland
+
                    microbial factory</h3>
−
                            China.</span></strong><a href="https://2021.igem.org/Team:CPU_CHINA/Map">click here to map</a></p>
+
 
−
                <p><span>&quot;ICII&quot; refers to &quot;Into China, Into iGEM&quot;. This is an Education &amp;
+
                <p>We hope to make primary school students know and be interested in synthetic biology, so we carried
−
                        Collaboration activity jointly sponsored by </span><strong><span>our team</span></strong><span>
+
                    out a popular science activity with the theme of microbial factory in Zaozhuang Primary School.
−
                        and
+
                    Since biology has not been systematically studied in primary school, we think we should choose more
−
                    </span><strong><span>NAU-China</span></strong><span>. 18 iGEM teams from all over China were invited
+
                    vivid ways of education.</p>
−
                        to
+
                <ol>
−
                        participate. As the </span><strong><span>18 teams</span></strong><span> were located in
+
                    <li>
−
                        different
+
                        <p>Members of CPU_CHINA recorded a popular science video for students to introduce the safety
−
                        provinces of China showed above, by motivating paticipant teams to deliver preachings regarding
+
                            regulations and layout of the laboratory and demonstrate the use of some instruments.</p>
−
                        synthetic biology and team projects at local middle schools or science and technology museums, a
+
                    </li>
−
                        national publicity of synthetic biology was achieved. During the preaching sessions held by
+
                    <li>
−
                        different teams in different locations, we encouraged the specific preaching team to display
+
                        <p>Our slides aimed to aid elementary school students in learning the basics of synthetic
−
                        project
+
                            biology. In addition, we also prepared microbial factory color cards to encourage them to
−
                        information and </span><strong><span>posters</span></strong><span> of other cooperant teams to
+
                            use their imagination to draw their own microbial factory. All the students gave us
−
                        realize a better exhibition effect. In addition, the exhibiting process of every team was
+
                            enthusiastic feedback and we received a total of 100 color cards.</p>
−
                        documented
+
                        <img src="https://static.igem.org/mediawiki/2021/6/6e/T--CPU_CHINA--Education_%26_Communication--Figure_2.jpg"
−
                        by video clips for the production of a final promotion
+
                            alt="">
−
                    </span><strong><span>vlog</span></strong><span>.</span></p>
+
                        <p class="imgdescribe"><strong>Fig. 2 Publicity site</strong></p>
−
                <p class="reference"><span>[1] Attachment: Posters made by </span><em><span>CPU_CHINA</span></em><span>
+
                        <img src="https://static.igem.org/mediawiki/2021/3/39/T--CPU_CHINA--Education_%26_Communication--Figure_3.jpg"
−
                         and
+
                            alt="">
−
                    </span><em><span>NAU-CHINA</span></em><span>; The vlog made by
+
                        <p class="imgdescribe"><strong>Fig. 3 Children&apos;s works</strong></p>
−
                     </span><em><span>CPU_CHINA</span></em>
+
                    </li>
−
                 </p>
+
                </ol>
−
                 <p class="reference"><span>[2] For further reading of ICII workflows and the preaching accomplished by
+
                <h3 class="mume-header" id="middle-school-synthetic-biology-and-igem">Middle school: synthetic biology
−
                        CPU_CHINA, see
+
                    and iGEM</h3>
−
                     </span><em><span>(Education website)</span></em></p>
+
 
 +
                <p>We hope that middle school students who already have a certain biological foundation can understand
 +
                    synthetic biology and iGEM, so we launched an online presentation to introduce them to synthetic
 +
                    biology and how it could be used in daily life.</p>
 +
                <ol>
 +
                    <li>
 +
                        <p>CPU_CHINA introduced the difference between synthetic biology and traditional biology and
 +
                            genetic engineering by means of a slide show, and connected them with real life. Our
 +
                            explanation aroused enthusiastic discussion among the students and received a great
 +
                            response.</p>
 +
                        <img src="https://static.igem.org/mediawiki/2021/9/91/T--CPU_CHINA--Education_%26_Communication--Figure_4.jpg"
 +
                            alt="">
 +
                        <p class="imgdescribe"><strong>Fig. 4 Into synthetic biology</strong></p>
 +
                    </li>
 +
                    <li>
 +
                         <p>We also showed students the creative inspiration and concept of <em>Meddy: Relight My
 +
                                Star</em> public art exhibition. We hope that through this further explanation, we can
 +
                            better publicize the art exhibition and let students understand its deeper meaning.</p>
 +
                     </li>
 +
                 </ol>
 +
                 <h3 class="mume-header" id="college-theory-and-practice">College: Theory and Practice</h3>
 +
 
 +
                <p>We cooperated with CPU Bio-X Club to carry out synthetic biology activities on campus.During the
 +
                    annual club carnival, we showed students of our project design and showed them the development
 +
                    potential of synthetic biology in various fields. At the same time, the members also introduced the
 +
                     hazards of white pollution to the students, reminding everyone to protect the environment from
 +
                    themselves and contribute to a better earth.</p>
 +
                <img src="https://static.igem.org/mediawiki/2021/5/5d/T--CPU_CHINA--Education_%26_Communication--Fig_4.png"
 +
                    alt="">
 +
                <p>We invited undergraduates from China Pharmaceutical University who are interested in synthetic
 +
                    biology to visit our lab. During that, we introduced them to our project and gave them some basic
 +
                    knowledge about synthetic biology. With the popularization of synthetic biology, we hope to attract
 +
                    more undergraduates to devote themselves to this field.</p>
 +
                <img src=" https://static.igem.org/mediawiki/2021/2/23/T--CPU_CHINA--Education_%26_Communication--Figure_5.jpg"
 +
                    alt="">
 
             </div>
 
             </div>
 +
            <div class="section" id="section4">
 +
                <h2 class="mume-header" id="promoting-garbage-classification">PROMOTING GARBAGE CLASSIFICATION</h2>
 +
 +
                <h3 class="mume-header" id="communityfun-knowledge-contest">Community&#xFF1A;Fun knowledge contest</h3>
 +
 +
                <p>In interviews with plastic recycling companies, they generally said that China&apos;s garbage sorting
 +
                    system still needs to be improved. Therefore, CPU_CHINA has carried out garbage classification
 +
                    publicity activities in Laiquan Community.</p>
 +
                    <img src="https://static.igem.org/mediawiki/2021/7/7b/T--CPU_CHINA--Education_%26_Communication--Figure_7.jpg" alt="">
 +
                    <p> We introduced to residents how to classify common garbage
 +
                    in daily life, and inform them of the threat that white pollution poses to the world. The promotion
 +
                    uses a combination of introduction and interesting Q&amp;A. The participant with the highest score
 +
                    will get the Meddy model carefully prepared by us, and all participants will give CPU_CHINA
 +
                    peripheral gifts.</p>
 +
                <h3 class="mume-header" id="rural-area-garbage-sorting-experience-center">Rural area: Garbage Sorting
 +
                    Experience Center</h3>
 +
 +
                <img src="https://static.igem.org/mediawiki/2021/5/57/T--CPU_CHINA--Education_%26_Communication--Figure_8.jpg"
 +
                    alt="">
 +
                <p>In order to achieve a wider range of popular science, we also went to the Zhangjiacun Garbage Sorting
 +
                    Experience Center in Shuangtang Community, established by the Nanjing Government, and participated
 +
                    in a variety of fun games in the experience center with the villagers. In addition to some fun games
 +
                    inside the experience hall, there is also a supermarket with a point reward system. The
 +
                    classification of other garbage and kitchen waste in residents&apos; homes is evaluated by
 +
                    collectors using smart devices, and the system automatically gives users different points. Residents
 +
                    can use the points to redeem small commodities in the point supermarket.After a day of experience
 +
                    and publicity, the concept of environmental protection has taken root in each of us.</p>
 +
            </div>
 +
            <div class="section" id="section5">
 +
                <h2 class="mume-header" id="meddyrelight-my-star-public-art-exhibition"><em>MEDDY&#xFF1A;RELIGHT MY
 +
                        STAR</em> <em>PUBLIC</em> ART EXHIBITION</h2>
 +
 +
                <p>Meddy&#xFF1A;Relight My Star, an exhibition in the form of <strong>public art</strong> showing how we
 +
                    are using <strong>synthetic biology</strong> to solve <strong>polyethylene plastic
 +
                        pollution</strong>. In this exhibition, everyone will play the role of an
 +
                    <strong>explorer</strong>, following in the footsteps of the protagonist, Meddy, to relight the star
 +
                    of synthetic biology.</p>
 +
                <img src="https://static.igem.org/mediawiki/2021/a/a6/T--CPU_CHINA--Education_%26_Communication--Fig_9.png"
 +
                    alt="">
 +
                <p><strong>Fig. MRMS synbio public art exhibition</strong></p>
 +
                <p>We use digital interactive animation, 3D printing, scrap, walls, floors, metal, lighting and other
 +
                    public art media to <strong>inspire people to think about the environmental crisis</strong> and to
 +
                    <strong>enable them to experience firsthand the magic of using synthetic biology to solve real world
 +
                        problems</strong>.</p>
 +
                <p>Click the button below for more details on a separate page.<a
 +
                        href="https://2021.igem.org/Team:CPU_CHINA/MeddyRelightMyStar">Team:CPU CHINA/MeddyRelightMyStar
 +
                        - 2021.igem.org</a></p>
 +
            </div>
 +
            <div id="attributions">
 +
                <a href="https://2021.igem.org/Team:CPU_CHINA/Attributions">ATTRIBUTIONS</a>
 +
            </div>
 +
            <div class="section" id="section6">
 +
                <h2 class="mume-header" id="meddy-in-wonderland-comic-strip">MEDDY IN WONDERLAND--COMIC STRIP</h2>
 +
 +
                <p><strong>Meddy In Wonderland</strong> is the guiding mini-comic of the exhibition, which tells the
 +
                    story of Meddy <strong>using synthetic biology to solve the polyethylene crisis</strong> . There are
 +
                    a lot of details about <strong>synthetic biology</strong> hidden in the comics that will help you
 +
                    understand and participate in the rest of the show. We believe that the wonderful pictures and
 +
                    interesting stories will attract the public, especially teenagers, and <strong>ignite interest in
 +
                        synthetic biology from a new perspective</strong>.</p>
 +
                <img src="https://static.igem.org/mediawiki/2021/0/08/T--CPU_CHINA--Education_%26_Communication--Fig_10.png" alt="">
 +
                <p class="imgdescribe"><strong>Fig. 5 Meddy in Wonderland synbio comic strip</strong></p>
 +
                <h3 class="mume-header" id="online-interaction">Online interaction</h3>
 +
 +
                <p>With the coming of the Internet era and the continuous development of new media, more and more
 +
                    audiences are changing their behavior patterns in life, work and study. New media, represented by
 +
                    the Internet and social media, progresses at an ever-high speed, constantly promoting the
 +
                    development and improvement of media.</p>
 +
                <img src="https://static.igem.org/mediawiki/2021/a/a6/T--CPU_CHINA--Education_%26_Communication--Fig_9.png" alt="">
 +
                <p>We seized this precious opportunity for new media development, and registered team accounts on some
 +
                    most popular social platforms such as MicroBlog and bilibili. Closely following trending topics, we
 +
                    updated scientific videos and tweets to interact with audiences from the local Science and
 +
                    Technology Museum, Teen Center, etc. A lot of teenagers made sense of iGEM and synthetic biology
 +
                    under our guidance through those platforms.</p>
 +
                <img src="https://static.igem.org/mediawiki/2021/9/9d/T--CPU_CHINA--Education_%26_Communication--Figure_11.jpg" alt="">
 +
            </div>
 +
 +
 +
 
         </div>
 
         </div>
−
    </div>
 
−
    </div>
 
 
     </div>
 
     </div>
 
     </div>
 
     </div>

Revision as of 20:56, 21 October 2021

INTRODUCTION

As an iGEMer, it is our responsibility to make synthetic biology more accessible and attractive. This year, our team recognized the limitations of traditional publicity activities which were restricted in small scopes or targeted on specialists who had already equipped with synthetic biology knowledge. Thus, after elaborate consideration, we launched ICII, a large-scale national publicity campaign, carrying out different science popularization and education activities for classified audience according to their age and level of education. CPU_CHINA also combined synthetic biology with other fields, further launched art exhibitions and produced comic strips. And for better giving publicity to synthetic biology and publishing popular science articles, we have created team accounts in bilibili, MicroBlog and other major social platforms, actively adapted to the wave of new media development.

ICII: SPONSOR OF JOINT EDUCATION ACTIVITIES

ICII, namely "Into CHINA, Into iGEM", is a summer tour activity to publicize synthetic biology and iGEM, jointly sponsored by CPU_CHINA and NAU-CHINA. As an Education&Collaboration activity hosted by two teams, 18 iGEM teams from all over the country were invited. The teams showed the project information and posters of all the cooperating teams when they preached in their locations, and shot vlogs to record the interesting process of preaching and urban culture.

Fig. 1 Poster of Into CHINA, Into iGEM

With the collaboration of multiple teams throughout the country, the impact of synthetic biology is hoped to be wider, broader and more profound. By approaching more cities and larger scale audiences, presenting new technology and new ideas in more specific forms, we aimed that our operation would achieve the common aspiration of projecting science popularization and iGEM event publicity, making synthetic biology more acceptable and impressive.

The sponsors compiled the vlogs of all the teams, and combined the presentation process and urban cultural publicity to edit a summary video. We hope to unfolded the culture of different cities in China to the iGEM world through the final video, showing the scientific and technological strength of China and the enthusiasm of the Chinese people for the development of biotechnology. Meanwhile, ICII could act as an entry point for the world to have a more comprehensive understanding of China.

Video 1. Vlog of Into CHINA, Into iGEM

These lectures are like a large-scale sowing, laying the seed of using synthetic biology to benefit society and change the world for children and adolescents. We are looking forward to their germination and growth tomorrow.

For more cooperation details, click the button below to view another separate page.

(Team:CPU CHINA/Collaborations - 2021.igem.org)

APPROACHING SYNTHETIC BIOLOGY

Primary school: Wonderful microbial factory

We hope to make primary school students know and be interested in synthetic biology, so we carried out a popular science activity with the theme of microbial factory in Zaozhuang Primary School. Since biology has not been systematically studied in primary school, we think we should choose more vivid ways of education.

  1. Members of CPU_CHINA recorded a popular science video for students to introduce the safety regulations and layout of the laboratory and demonstrate the use of some instruments.

  2. Our slides aimed to aid elementary school students in learning the basics of synthetic biology. In addition, we also prepared microbial factory color cards to encourage them to use their imagination to draw their own microbial factory. All the students gave us enthusiastic feedback and we received a total of 100 color cards.

    Fig. 2 Publicity site

    Fig. 3 Children's works

Middle school: synthetic biology and iGEM

We hope that middle school students who already have a certain biological foundation can understand synthetic biology and iGEM, so we launched an online presentation to introduce them to synthetic biology and how it could be used in daily life.

  1. CPU_CHINA introduced the difference between synthetic biology and traditional biology and genetic engineering by means of a slide show, and connected them with real life. Our explanation aroused enthusiastic discussion among the students and received a great response.

    Fig. 4 Into synthetic biology

  2. We also showed students the creative inspiration and concept of Meddy: Relight My Star public art exhibition. We hope that through this further explanation, we can better publicize the art exhibition and let students understand its deeper meaning.

College: Theory and Practice

We cooperated with CPU Bio-X Club to carry out synthetic biology activities on campus.During the annual club carnival, we showed students of our project design and showed them the development potential of synthetic biology in various fields. At the same time, the members also introduced the hazards of white pollution to the students, reminding everyone to protect the environment from themselves and contribute to a better earth.

We invited undergraduates from China Pharmaceutical University who are interested in synthetic biology to visit our lab. During that, we introduced them to our project and gave them some basic knowledge about synthetic biology. With the popularization of synthetic biology, we hope to attract more undergraduates to devote themselves to this field.

PROMOTING GARBAGE CLASSIFICATION

Community：Fun knowledge contest

In interviews with plastic recycling companies, they generally said that China's garbage sorting system still needs to be improved. Therefore, CPU_CHINA has carried out garbage classification publicity activities in Laiquan Community.

We introduced to residents how to classify common garbage in daily life, and inform them of the threat that white pollution poses to the world. The promotion uses a combination of introduction and interesting Q&A. The participant with the highest score will get the Meddy model carefully prepared by us, and all participants will give CPU_CHINA peripheral gifts.

Rural area: Garbage Sorting Experience Center

In order to achieve a wider range of popular science, we also went to the Zhangjiacun Garbage Sorting Experience Center in Shuangtang Community, established by the Nanjing Government, and participated in a variety of fun games in the experience center with the villagers. In addition to some fun games inside the experience hall, there is also a supermarket with a point reward system. The classification of other garbage and kitchen waste in residents' homes is evaluated by collectors using smart devices, and the system automatically gives users different points. Residents can use the points to redeem small commodities in the point supermarket.After a day of experience and publicity, the concept of environmental protection has taken root in each of us.

MEDDY：RELIGHT MY STAR PUBLIC ART EXHIBITION

Meddy：Relight My Star, an exhibition in the form of public art showing how we are using synthetic biology to solve polyethylene plastic pollution. In this exhibition, everyone will play the role of an explorer, following in the footsteps of the protagonist, Meddy, to relight the star of synthetic biology.

Fig. MRMS synbio public art exhibition

We use digital interactive animation, 3D printing, scrap, walls, floors, metal, lighting and other public art media to inspire people to think about the environmental crisis and to enable them to experience firsthand the magic of using synthetic biology to solve real world problems.

Click the button below for more details on a separate page.Team:CPU CHINA/MeddyRelightMyStar - 2021.igem.org

MEDDY IN WONDERLAND--COMIC STRIP

Meddy In Wonderland is the guiding mini-comic of the exhibition, which tells the story of Meddy using synthetic biology to solve the polyethylene crisis . There are a lot of details about synthetic biology hidden in the comics that will help you understand and participate in the rest of the show. We believe that the wonderful pictures and interesting stories will attract the public, especially teenagers, and ignite interest in synthetic biology from a new perspective.

Fig. 5 Meddy in Wonderland synbio comic strip

Online interaction

With the coming of the Internet era and the continuous development of new media, more and more audiences are changing their behavior patterns in life, work and study. New media, represented by the Internet and social media, progresses at an ever-high speed, constantly promoting the development and improvement of media.

We seized this precious opportunity for new media development, and registered team accounts on some most popular social platforms such as MicroBlog and bilibili. Closely following trending topics, we updated scientific videos and tweets to interact with audiences from the local Science and Technology Museum, Teen Center, etc. A lot of teenagers made sense of iGEM and synthetic biology under our guidance through those platforms.