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| − | <!--内容-->
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| − | <div class="content-middle-outer">
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| − | <div class="content-middle">
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| − | <section class="article p-t-30 p-b-54">
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| − | <h1 class="content-header">Notebook</h1>
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| − | <table>
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| − | <tr>
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| − | <td colspan="2" class="notebook-head">July</td>
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| − | </tr>
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| − | <tr>
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| − | <td style="width: 20%">4<sup>th</sup></td>
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| − | <td>Prepare culture mediums.</td>
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| − | </tr>
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| − | <tr>
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| − | <td>5<sup>th</sup></td>
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| − | <td>Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA.</td>
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| − | </tr>
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| − | <tr>
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| − | <td>6<sup>th</sup></td>
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| − | <td>Construction of the 4 plasmids.</td>
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| − | </tr>
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| − | <tr>
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| − | <td>7<sup>th</sup></td>
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| − | <td>Colony PCR, gel electrophoresis, and select single clones.</td>
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| − | </tr>
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| − | <tr>
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| − | <td>8<sup>th</sup></td>
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| − | <td>Plasmids extraction from E. Coli, and sent to the DNA sequencing company.</td>
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| − | </tr>
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| − | <tr>
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| − | <td>9<sup>th</sup></td>
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| − | <td>Verify the DNA sequencing resuls and carry out the transformation of plasmids into the
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| − | Saccharomyces cerevisiae by Heat-Shock approach.
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| − | </td>
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| − | </tr>
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| − | <tr>
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| − | <td>11<sup>st</sup></td>
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| − | <td>Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in
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| − | 1ml culture mediums.
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| − | </td>
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| − | </tr>
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| − | <tr>
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| − | <td>12<sup>nd</sup></td>
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| − | <td>Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate
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| − | efficient growth. Hence, we were not able to carry out the enzyme acitivety tests.
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| − | </td>
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| − | </tr>
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| − | <tr>
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| − | <td>13<sup>th</sup></td>
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| − | <td>Carry out fermentation performance test by testing the concentrations of glucose and ethanol.
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| − | </td>
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| − | </tr>
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| − | <tr>
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| − | <td colspan="2" class="notebook-head">August</td>
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| − | </tr>
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| − | <tr>
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| − | <td colspan="2">Repeat the plasmids construction experiments and the yeast culture by AQ yeast.</td>
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| − | </tr>
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| − | <tr>
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| − | <td colspan="2" class="notebook-head">September</td>
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| − | </tr>
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| − | <tr>
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| − | <td colspan="2">Carry out enzyme activity tests and fermentation performance tests.</td>
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| − | </tr>
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| − | </table>
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| − | </section>
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