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| − | <!--内容-->
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| − | <section class="article p-t-30 p-b-54">
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| − | <h1 class="content-header">Communication</h1>
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| − | | + | |
| − | <section>
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| − | <p>We introduced CRISPR technology and PCR technology to our community in a PowerPoint presentation and
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| − | a recorded video. The majority of our educational audience were children. Therefore, we streamlined
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| − | the PowerPoint and video instruction to make it easier to understand. Initially, we included a
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| − | detailed description of CRISPR and PCR technologies in the PowerPoint. All of the materials
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| − | mentioned in the PPT are told with vivid cartoon and pictures. <span style="color: red">Here attached the educational
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| − | PPT.</span></p>
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| − | <img class="m-t-12" src="https://static.igem.org/mediawiki/2021/b/bc/T--Xiamen_City--img_communication_2.jpg" alt="" style="width: 70%;">
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| − | <video src="https://static.igem.org/mediawiki/2021/6/6d/T--Xiamen_City--video_communication.mp4"
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| − | controls preload="metadata"
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| − | class="m-t-24 m-b-12"
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| − | style="width: 100%; height: 100%">
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| − | </video>
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| − | <p>In these material, we showed children process of yeast decomposition and the importance of
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| − | multifunctional yeast in the winemaking. We also explained gene-editing technology in the simplest
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| − | terms. We gave the children some knowledge about the structure of plant roots, stems and leaves.
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| − | Dividing the children into groups, we organized the students to work together to put together the
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| − | root and leaf structure of plants, which brought them a lot of joy. </p>
| + | |
| − | <p>We have also set up our WeChat official account as an important communication channel with the
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| − | public. Initially, the account functions as a recorder of what we have done. As our followers
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| − | increased rapidly, we found it a great means to educate the public about gene-editing technique. We
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| − | have published 7 articles and some of them have been read by over 1000 people.</p>
| + | |
| − | <img class="m-t-12" src="https://static.igem.org/mediawiki/2021/0/07/T--Xiamen_City--img_communication_3.jpg" alt="" style="width: 70%;">
| + | |
| − | <img class="m-t-18" src="https://static.igem.org/mediawiki/2021/6/6c/T--Xiamen_City--img_communication_5.jpg" alt="" style="width: 70%;">
| + | |
| − | <p>As we have rent an office to work together in a crowded office building, we took advantage of great
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| − | human traffic in the morning and evening peak. We designed a roll up banner and placed it in front
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| − | of our office, and took turns to hand out brochures with our official account's QR code of our
| + | |
| − | official account. To avoid people's aversion, we also handed out free masks with our cute logo on
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| − | it. By doing this, our official account obtained a great number of new followers.</p>
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| − | <img class="m-t-12" src="https://static.igem.org/mediawiki/2021/2/29/T--Xiamen_City--img_communication_4.jpg" alt="" style="width: 50%;">
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| − | | + | |
| − | <p>In order to enlarge our impact, we intend to make a MV for our team. We rewrote the lyric of STAY(
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| − | from the Kid LAROI/Justin Bieber). We talked about our development procedures of designing
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| − | multifunctional yeast and our future market entrance plan. We are still designing and making the
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| − | final video. We plan to release the video on several popular platforms, such as YouTube, Bilibili
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| − | and Weibo.</p>
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| − | | + | |
| − | <br>
| + | |
| − | <p>Here attached the lyric ( we recompose the lyrics).</p>
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| − | | + | |
| − | <section style="white-space: pre-line; font-size: 16px">
| + | |
| − | <section style="color: #008AC6">We do the same, thing we planned that we had to do.
| + | |
| − | | + | |
| − | We tried our best, even if it's hard to do we
| + | |
| − | | + | |
| − | never regret any decisions we have made
| + | |
| − | | + | |
| − | Cuz it worth it,it worth it, hey
| + | |
| − | </section>
| + | |
| − | I'm tired of, Wiki and Business plan
| + | |
| − | | + | |
| − | I realize the time that I wasted here
| + | |
| − | | + | |
| − | I feel like I can't feel the light of life
| + | |
| − | <section style="color: #008AC6">
| + | |
| − | i.G.E.M. is what we're striving for.
| + | |
| − | | + | |
| − | Oh, ooh-woah (Oh, ooh-woah)
| + | |
| − | | + | |
| − | Oh, ooh-woah (Oh, ooh-woah)
| + | |
| − | | + | |
| − | Oh, ooh-woah (Oh, ooh-woah)
| + | |
| − | | + | |
| − | i.G.E.M. is what we're striving for.
| + | |
| − | | + | |
| − | We do the same, thing we planned that we had to do.
| + | |
| − | | + | |
| − | We tried our best, even if it's hard to do we
| + | |
| − | | + | |
| − | never regret any decisions we have made
| + | |
| − | | + | |
| − | Cuz it worth it,it worth it, hey
| + | |
| − | </section>
| + | |
| − | We are the pecteast and we work on that(Ooh)
| + | |
| − | | + | |
| − | It is the reason that we gathered round(Ooh)
| + | |
| − | | + | |
| − | It's been difficult for us to trust (Ooh)
| + | |
| − | | + | |
| − | That we've already finished all the stuffs(Ooh)
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| − | | + | |
| − | Ain't no way that I can leave you pecteast
| + | |
| − | | + | |
| − | 'Cause you ain't ever left me empty-handed
| + | |
| − | | + | |
| − | And you know that I know that I can't live without you
| + | |
| − | | + | |
| − | So, pecteast, stay
| + | |
| − | <section style="color: #008AC6">
| + | |
| − | Oh, ooh-woah (Oh, ooh-woah)
| + | |
| − | | + | |
| − | Oh, ooh-woah (Oh, ooh-woah)
| + | |
| − | | + | |
| − | Oh, ooh-woah (Oh, ooh-woah)
| + | |
| − | | + | |
| − | i.G.E.M. is what we're striving for.
| + | |
| − | | + | |
| − | We do the same, thing we planned that we had to do.
| + | |
| − | | + | |
| − | We tried our best, even if it's hard to do we
| + | |
| − | | + | |
| − | never regret any decisions we have made
| + | |
| − | | + | |
| − | Cuz it worth it,it worth it, hey
| + | |
| − | | + | |
| − | We do the same, thing we planned that we had to do.
| + | |
| − | | + | |
| − | We tried our best, even if it's hard to do we
| + | |
| − | | + | |
| − | never regret any decisions we have made
| + | |
| − | | + | |
| − | Cuz it worth it,it worth it, hey
| + | |
| − | </section>
| + | |
| − | </section>
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| − | | + | |
| − | <p>In addition, to make the process of knowing synthetic biology intriguing, our team designs an
| + | |
| − | aeroplane chess map. The rules are similar to the original ones. We make some changes in the layout
| + | |
| − | of our map and the ‘ accident’ cards. Here attached our game.</p>
| + | |
| − | </section>
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| − | | + | |
| − | <section>
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| − | <h1 class="title">Rules:</h1>
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| − | <p>DNA: (HEREDITY) The next round will have the same number of points as this round</p>
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| − | <p>RNA: (HEREDITY) Subtract 1 step per round</p>
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| − | <p>CRISPR-CAS9: Subtract the number of steps in the next round from the number of steps in the current
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| − | round. Take the absolute value if the number of steps in the next round is less.</p>
| + | |
| − | <p>Vaccines: Immunize all effects（e.g. ｜6-5｜=1 or ｜5-6｜=1）</p>
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| − | <p>Saccharomyces Cerevisiae: (Fermentation) Double the number of points in the next round</p>
| + | |
| − | <p>Cell Division: Stepping on a bond piece will have a split function. When stepped on by other bonds
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| − | won’t die, instead of going to the starting point. It can be split into 2 small bond, but both of
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| − | them can only be counted as one.</p>
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| − | <p>Pectase: (CATALYSIS) Roll the dice twice in the next round, help to get to terminus faster.</p>
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| − | <p>Pectin: (VISCIDITY) Get stuck in place one round</p>
| + | |
| − | <p>Virus: Has the ability of attacking other players</p>
| + | |
| − | <ol>
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| − | <li><p>one player can be designated to take one step back each turn</p></li>
| + | |
| − | <li><p>overtake a designated player</p></li>
| + | |
| − | </ol>
| + | |
| − | </section>
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| − | | + | |
| − | <section>
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| − | <h1 class="title">Map:</h1>
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| − | <img class="m-b-12" src="https://static.igem.org/mediawiki/2021/4/49/T--Xiamen_City--img_communication_6.jpg" alt="" style="width: 50%;">
| + | |
| − | | + | |
| − | <p>To further enhance our influence, we planned to contribute and publish our research paper on public
| + | |
| − | education website <i>Questioz</i>, our abstract are followings:</p>
| + | |
| − | </section>
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| − | | + | |
| − | <section>
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| − | <h1 class="title">Background:</h1>
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| − | <p>During pandemic, transportation and trading has been greatly blocked . As commodity are deteriorating
| + | |
| − | rapidly, fruit market has been greatly impacted. Many fruits were heavily stagnated and fruit
| + | |
| − | farmers lost stable income. This study produces a new saccharomyces cerevisiae that might offers a
| + | |
| − | solution to this problem.</p>
| + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
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| − | <h1 class="title">Methods: </h1>
| + | |
| − | <p>After two rounds of previous research, interviewing several bioengineering and zymurgy experts, we
| + | |
| − | found this problem can be solved indirectly in biological method. Researchers are looking forward to
| + | |
| − | genetically-edit saccharomyces cerevisiae so it acquires pectinase’s function. This new yeast is
| + | |
| − | expected to cut fruit wine production cost in order to lower wine industry’s barrier and finally
| + | |
| − | increase fruit demand. </p>
| + | |
| − | <p>Using CRISPR-Cas9 technology, the heterologous gene expression process of endo-PGAA gene from
| + | |
| − | Aspergillus Niger SC323 in wine yeast was introduced into phcas9-Nours plasmid containing Cas gene.
| + | |
| − | Then, the pYES2 -- GRNA-HYg-MCS plasmid carrying sgRNA (anchoring HXK1) and the homologous arm for
| + | |
| − | repair were introduced into the fruit wine yeast. Lastly, the transposers were obtained to verify
| + | |
| − | whether endo-PGAA was replaced to the HXK1 gene site.</p>
| + | |
| − | <p>The process of verifying the ability of saccharomyces cerevisiae to produce pectinase are followings.
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| − | Bromophenol blue medium is used to verify whether the integrated strain produced pectinase. The
| + | |
| − | integrated strains are inoculated to 30°C incubator and cultured for 3 days. If we found that the
| + | |
| − | integrated strains on bromophenol blue medium had obvious yellow circles, while the wild type
| + | |
| − | strains did not, we can indicate that endo-PGAA gene has been successfully introduced into
| + | |
| − | saccharomyces cerevisiae.</p>
| + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
| + | |
| − | <h1 class="title">Results:</h1>
| + | |
| − | <p>The experiment turned out to be successful. The final product can degrade pectin well and replace
| + | |
| − | pectin in wine production process. Researchers are looking forward to commercializing it in the near
| + | |
| − | future.</p>
| + | |
| − | </section>
| + | |
| − | | + | |
| − | <section>
| + | |
| − | <h1 class="title">Here attached some question samples we used while we are interviewing with professors
| + | |
| − | and passing-by.</h1>
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| − | <ol>
| + | |
| − | | + | |
| − | <li><p>What do you think of the recent wine market? What about fruit wines?</p></li>
| + | |
| − | <li><p>Is there any national policy (regulation) on the wine market now? (Recently)</p></li>
| + | |
| − | <li><p>Do you think wineries will buy our yeast?</p></li>
| + | |
| − | <li><p>How to deal with expired fruit wines?</p></li>
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| − | <li><p>Will it affect the stagnant fruits?</p></li>
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| − | <li><p>Is it costly to make fruit wine now?</p></li>
| + | |
| − | <li><p>What do you think about gene editing technology (are you willing to consume genetically
| + | |
| − | edited food?)</p></li>
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| − | <li><p>Do you know the difference between fruit wine and other wines?</p></li>
| + | |
| − | <li><p>How often do you usually drink wine?</p></li>
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| − | <li><p>What do you think is the impact of using gene editing technology to reduce the price of fruit
| + | |
| − | wines on the sales of fruit wines: good/bad/not much?</p></li>
| + | |
| − | </ol>
| + | |
| − | </section>
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| − | </section>
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| − | </div>
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| − | </div>
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| − | <!--Footer-->
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| − | <div class="footer">
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| − | <div class="content">
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| − | <h2 style="font-size: 28px; margin-bottom: 12px !important;">Contact Info</h2>
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| − | <img src="https://static.igem.org/mediawiki/2021/b/ba/T--Xiamen_City--qrcode.jpg" alt="" style="width: 200px">
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| − | <div style="margin-top: 12px"><i><span>WeChat Official Account:</span><span style="color: #00177a"> iGEM PecTeast</span></i>
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| − | </div>
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| − | </html>
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