Team:Rio UFRJ Brazil/Notebook

Notebook



A long journey


Osiris Rio UFRJ team was founded in 2017 with the goal of being the first Synthetic Biology team from the State of Rio de Janeiro, Brazil to participate in the iGEM competition. Since then we had many challenges to make this dream come true: assembling a team, finding a Principal Investigator to support us and finally accomplishing financial aid to get us to the competition, which for a long time was the main bottleneck that prevented us from participating in iGEM before.

Considering we were trying to participate in the competition for such a long time, we have accomplished a few things even before the year of the competition. From the achievement of the funding to participate in the competition until the final weeks before the wiki freeze, we have endured a long journey, with highs and lows, but overall very knowledgeable and fun! Check out below our main milestones!




2020


Finally received the investment from the government!

  • In 2019 we received governmental aid through a Parliamentary Amendment granted by the Brazilian political party Novo to fund our diagnostic project aimed at arboviral infections detection. However we only received it in 2020, but not in time to participate in this year’s edition.


Idea brainstorm

  • We had many ideas since the creation of the team, all with the goal to mitigate the problems associated with arboviral infections misdiagnosis. But only in 2020 we established the present concept of how we would address this problem: using multi-epitope proteins.


Other achievements

  • Initial conversation with professors about protein and its expression system, immunology and biosensors


  • Multi-epitope protein initial design steps and modeling


  • Multi-epitope synthesis by GenScript Biotech


  • DME-BR purified by our partner


2021


January

  • Expression and solubility test of DME-C (DME-C was fortunately on soluble fraction!)


February

  • Due to an increase in the pandemic cases, access to the laboratory was reduced and we had to interrupt the experiments.


March

  • DME-C expression in higher volume.


April

  • iGEM Registration Fee payment!


May

  • DME-C Histrap chromatography
  • We finally purified the protein! However, we still needed to refine the purification.

  • First meeting with our iGEM mentor!
  • We are participating in the iGEM mentorship program and finally, in May we had our first meeting with Jorge Luis Hernandez Ayal, our mentor!

  • Starting to write the Safety Forms

  • Initial interviews with physicians


June

  • Working on the Promo Video!

  • Issues with the protein concentration determination method

  • We had tried using different methods (Bradford, Lowry) but it took us some time to realize that the imidazole used in the purification method was interfering with our measurements.
  • Talking with the Calgary team!

  • First talk with the Queens team!

  • Building the forms to evaluate the arboviral infection diagnostics problem among other physicians.

  • Talk with a specialist from a Diagnostic Clinic


July

  • Final Promo Video Editing!

  • Started to build our business model

  • First talk with the Rochester team!


August

  • Participation in the Brazilian Symposium on Synthetic Biology!
  • Start working on the wiki!
  • The Queens team helped us to understand our docking results.
  • Diafiltration of DME-C to remove Imidazole.
  • Our team was selected for the iGEM Impact Grant!
  • With the funding we were able to pay the Giant Jamboree fee




September

  • Western blots and ELISA assays anti-Dengue to check our protein’s ability to recognize anti-dengue antibodies

  • Participation in iGEM LATAM fest!


October

  • Western blots and ELISA assays anti-Zika to evaluate cross reaction.

    • Wiki final edition

    • Final discussions about the partnership with the Rochester team






  • Sponsers & Partnerships
    Contact
    osirisrioufrj@gmail.com
    Avenida Carlos Chagas Filho, 373, Bloco D, Subsolo, Sala SS-11
    Cidade Universitária, Rio de Janeiro - RJ, 21941-902