Team:Rio UFRJ Brazil/Contribution


Page's Problem

Lack of information about linkers registered in iGEM

In our project we designed a chimera protein, Ammit, which contains different Dengue epitopes. In chimeric proteins, several pieces of polypeptides, from different origins, are joined together. In order to separate these multiple protein domains, in natural proteins, spacers or linkers are used. These linkers can adopt different structures or functions, so the choice for the best linkers is an essential step involved in the design of the chimeric proteins. To choose the best linkers for Ammit we performed an extensive bibliographic research, and searched the iGEM website to study previous work related to this subject. We found a page on the iGEM website that specifically talks about linkers that could aid the ligation between the different epitopes. The linkers contained basic but important information, such as its constitution and brief description, however some lack important information such as functionality, structural characteristics, and applicability. This information is extremely important to support studies related to the creation of chimeric proteins and other related research.


Additional information about linkers registered in iGEM

Given the importance of a more detailed description of linkers, the Osiris team chose four different linkers and decided to study and add to the website their characteristics in more detail, such as functionality and applicability. The chosen linkers were: BBa_J176131, BBa_K1486004, BBa_K648006 and BBa_K648007. All are represented with their respective iGEM codes, registered on the linkers page (access here).

The team researched their descriptions, applicability, functionality, molecular data, etc. With this, we were able to find research carried out in scientific articles and patented works. We then added, for each one, a description aimed at its use in research projects, as well as its characteristics and interaction. In addition, we added the molecular weight of the proteins and their isoelectric point to help in the development of future scientific work involving the linkers in question.

We hope that with this contribution, we can help future teams with the development of their work and research. Access below the page of each of the linkers with their additional descriptions:

Chimeric Protein Guide

Construction of chimeric proteins

During the creation and development of Ammit, the team identified some important points that must be taken into account when assembling and creating chimeric proteins. These points involve choosing sequences for joining, linker definition, structural modeling, docking simulation, and cloning. Therefore, we decided to build a Chimeric Protein Guide to help and facilitate work developed by future iGEM teams, involving all the points mentioned above. After a conversation with the team Queens, the team suggested addressing some other topics such as 3D protein structures and post-translational modifications. Since their work also involves the construction of chimeric proteins.

Below is the link to download the Chimeric Protein Guide, click and access for important information on the subject:
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