Notebook
DRY LAB NOTEBOK
7/29
-Made some icebreakers to help team members get known to each other and build friendship and team soul
-iGEM opening report meeting
-Introduction to IBD
8/12
-Determination of team name
-Team logo design
-Team caption selection
-Selection of team products
-Online expert interview
8/13
-Online survey production
8/14
-WeChat tweet writing
09/19
-Introduction of the subject
-Completed the project PPT production
-Discussion of analytical questionnaire online
-Design of expert interview questions
-Discussion of sales of team derivatives'
09/20
-Message broadcasting on WeChat
-Pricing of team products
-Team collaboration
-Team member' self introduction writing
10/1
-Team photo shooting
10/2
-Collaboration Meetups
10/3
-Overall review of the experiment
10/4
-Wiki material writing
10/5
- Expert Interview
WET LAB NOTEBOOK
2021.9.11
1. Bacteria expressed pUC57-amilGFP/pSU2718-p15A activated.
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2. Plasmid transformation into Top 10 competent cell
Plasmid A: pUC57-PyeaR_HrpR
Plasmid B: pUC57-PttB344_HrpS_PJ23105_ttrR
Plasmid C: pUC57(Kan)-PhrpL_amilGFP_IL10_PJ23104_ttrS
2021.9.12
1. Extracted plasmid pUC57-amilGFP and pSU2718-p15A
2. Enzyme digestion (Plasmid pSU2718-p15A)
3. DNA agarose gel electrophoresis identification
4. DNA agarose gel reavers and purification
2021.9.13
1. Extracted plasmid pUC57-PyeaR_HrpR, pUC57-PttB344_HrpS_PJ23105_ttrR and pUC57(Kan)-PhrpL_amilGFP_IL10_PJ23104_ttrS
2. PCR to get part 2 (PttB344_HrpS_PJ23105_ttrR)
3. DNA agarose gel electrophoresis identification
4. DNA agarose gel reavers and purification
5. T4 ligase link digested plasmid pSU2718-p15A and part2 PttB344_HrpS_PJ23105_ttrR
6. Recombinant plasmid transformation
2021.9.14
-Use micropipette tips to pick a single colony from the plate (from day 9.13), put it in LB liquid medium.
2021.9.15
1. Plasmid pSU2718-Part2 extraction
2. Enzyme digestion identification
3. Plasmid was sent to sequence
2021.9.17
Correct plasmid pSU2718-Part2 extraction
2021.9.18
1. Enzyme digestion (Plasmid pSU2718-Part2)
2. PCR to get part 1 (PyeaR_HrpR)
3. DNA agarose gel electrophoresis identification
4. DNA agarose gel reavers and purification
5. T4 ligase link digested plasmid pSU2718-Part2 and part1 PyeaR_HrpR
6. Recombinant plasmid transformation
2021.9.19
Bacteria PCR identification
2021.9.20
1. Plasmid pSU2718-Part2-Part1extraction
2. Enzyme digestion identification
3. Positive plasmid was sent to sequence
2021.9.22
Plasmid transformation (pUC57(Kan)-PhrpL_amilGFP_IL10_PJ23104_ttrS) into Top 10 cell.
2021.9.24
1. Prepare Top10/pUC57(Kan)-PhrpL_amilGFP_IL10_PJ23104_ttrS competent cell；
2. Plasmid (pSU2718-Part2-Part1) transformation into Top10/pUC57(Kan)-PhrpL_amilGFP_IL10_PJ23104_ttrS competent cell
2021.9.25
Use micropipette tips to pick a single colony from the plate (from day 9.24), put it in LB liquid medium.
2021.9.26
Plasmid extraction.
2021.10.1-2021.10.7
Function tests