Team:IISER-Pune-India/TeamNotebook/Team Notebook 11e9906b405543a29361e38a50c81024/Meeting with Dr Annamma 2 with Reena Pandit 214d46d3860e4d4d8163844d8628b1f7

Meeting with Dr. Annamma 2 with Reena Pandit

Meeting with Dr. Annamma 2 with Reena Pandit

DescriptioniHP on butanol, industry
HP sub-branchiHP
Property 1
Property 2

Questions to ask:

How do we find the optimal frequency of extraction of butanol if we do continuous extraction?

What butanol assay to design to compare butanol production with and without immobilization?

Will the butanol go back into the cell if it stays in the media for a long time and how much?

Butanol toxicity for cyano? Will immobilization help? If butanol is toxic to cyano how do we deal with that?

If our supernatant has equal parts of butanol ethanol, and some pyruvate, do there exist economically viable ways to separate these products

Relevance of feedback inhibition and its effect on butanol production



  • Pick your words properly - a piece of general advice
  • Butanol extraction- minimum inhibitory concentration- Dr Sham's papers should have assays to measure MIC
  • Ann- Glucose kinase is a membrane protein. Are there soluble proteins in the cytoplasm? Is hexokinase available in the cytoplasm?
  • How much CO2 can Synechococcus uptake? There are two components to CO2 uptake - solubility of CO2 in medium and absorption from solvent
  • Algae can tolerate up to 20, 30 or even 50% CO2
  • Gas analyzer would be required for measuring CO2 consumption.
  • Please do not believe high values of CO2 to sucrose conversion. Send Reena Pandit the papers.
  • S. elongatus can grow under sunlight
  • Why did we not choose cyanobacteria to make the final product? - E coli is well documented. Too much stress on the system.
  • Coculture dynamic is very crucial. Please model it.
  • The cyanobacteria will find the butanol toxic. Use continuous extraction. ASK ANAMMA
  • Apart from sucrose, there are other nutrients needed by E coli to grow. The minor media components will get exhausted easily (eg: P). So these will not be available for E coli.
  • We will need to replace a few components of the media.
  • We could adapt E coli in BG-11
  • Caution: O2 build up- Cyano produces O2 at high rate under natural light. This can be toxic to E coli.
  • Micro aerobic conditions will work well at low light intensities.
  • Our species is robust and we should aim at doing this with natural light. This isn't possible right now but project it as it can take natural light - SUSTAINABLE
  • Flue gas is not sustainable. We have to add pipelines, cool it, it isn't very soluble. Impurities aren't a big problem.
  • There are membrane-based processes that concentrate CO2 in situ.
  • Sewage water doesn't have enough nutrients to support bacteria growth.
  • Cyanobacteria is a fertilizer. But the co-coculture cannot go as fertilizer or feed.
  • Don't immobilize cyanobacteria because light might not travel.
  • Look at separation strategies for butanol, ethanol and pyruvate separation.
  • We look at cell number or nitrate consumption to test the effects of butanol, ethanol or pyruvate on growth
  • Have a specific line of thought, and list its pros and cons
  • Culture the two parts separately and after sucrose is produced take that medium and put it into e coli and bubble oxygen and check if E coli can survive the pressure.
  • Campaign for HP
  • Water usage in growing sugar for generating sucrose vs cyanobacteria producing sucrose
  • We could set a threshold level of sucrose, and if we reach that level with cyanobacteria then our project is viable.