Team:IISER-Pune-India/TeamNotebook/Team Notebook 11e9906b405543a29361e38a50c81024/Dry Lab General Meeting 2 e53c61801433449a956bffbadd41c7fd

Dry Lab General Meeting 2

Dry Lab General Meeting 2

DepartmentDry Lab
DescriptionMeeting to gauge understanding of FBA and to decide which models to focus on for now
HP sub-branch
Property 1
Property 2

We have completed last week's checklist except for viability of co-culture FBA.


  • The wet lab is still working on which co culture method to use. Available options :
    1. Encapsulation
    1. Hydrogel Polymer

    Depending on what they choose to use we will have to change our approach to modelling (fine tune not change the whole approach).

  • The wet lab is yet to choose strains to work with, but the most likely choices are PCC 7942 and UTEX 2973, and since PCC 7942 already as a model in BiGG we decided to create a model for UTEX 2973 using the excel sheet below:

    BiGG Model for PCC 7942 -

  • We will have to find gene correspondence for the UTEX model using the model for PCC 6803, which is what the UTEX model was derived from BRUTE FORCE URGH!
  • Convert above model into SBML
  • The Taguchi method for optimising the culture medium is very wet lab intensive and so we have decided to find a dry lab alternative for the same. This is most probably going to consist of performing statistical analysis on a literature values, but that is just a guess for now.
  • Promoter Modelling is something to look into but not rn cuz that shit HARD BRUH

Division of Work

  • Arya, Misaal, Jason - CoCulture FBA - Wednesday
  • Arsh, Soorya - Taguchi method alternative (dry lab)
  • Soorya, Namasi - Modelling coculture medium