Wet lab Meeting - 3 with PhD mentors
| Date | |
|---|---|
| Department | Wet Lab |
| Description | Discussed queries regarding proposal with PhD mentors |
| HP sub-branch | |
| Links/media | |
| Participants | |
| Property | |
| Property 1 | |
| Property 2 | |
| Type | Meetings |
Things to do:
1) come up with schematics of construct 2) Plasmid maps 3) genes in order of interest 4) List down co-culture assays 5) best case scenerio - HK27659k gifted from the author, next best case scenario - first knock out poxB... then others later
- focus more on dry lab
- try doing only the essential modifications in e coli because 9 modifications is too much
- or else try contact authors for plasmids they used
- focus more on showing that the coculture works
- mohit is the only one with some experience with CRISPR he's made a fifes guide RNAs but that's all
- they asked us to find out all the plasmids being used for all modifications and have the genotypes of the strains used
- and also all the plasmid maps
- and after that we could ask them specific questions they could help us out with over shorter meetings
Realised that Zhang (2020) 3-HP paper obtained higher sucrose production rates than the encapsulation methods