Meeting with Rushik - 4
| Date | |
|---|---|
| Department | Wet Lab |
| Description | Explanation on wet lab techniques |
| HP sub-branch | |
| Links/media | |
| Participants | |
| Property | |
| Property 1 | |
| Property 2 | |
| Type | Meetings |
Rushik explained RF cloning
PFU polymerases
Dpn1 - to digest original bacterial plasmid which is methylated, the amplified, modified DNA after PCR is unmethylated and hence is not destroyed
RO water, double deionized water - milli-Q
pET28 → contains kanR
we could try to obtain specR gene from Sunish
Safety form:
acrylamide for SDS page is a neurotoxin and is carcinogenic
EtBr for staining gel is mutagen
TEMED used to polymerise acrylamide for SDS page is toxic
2021 batch of iPhD/PhD students are coming to campus on 2nd July