Meeting with Rushik - 3
| Date | |
|---|---|
| Department | Wet Lab |
| Description | Meeting with Rushik to discuss constructs. |
| HP sub-branch | |
| Links/media | |
| Participants | |
| Property | |
| Property 1 | |
| Property 2 | |
| Type | Meetings |
- Get quotations of pCSCX from addgene
- Clarify bom site sequence and its presence in Psyn1 and Psyn6 vectors with Prem
- Email nishad about E coli W and IPTG inducible plasmids containing specR or KanR
- complete safety form
- ask for pSyn1 map from prem
- protocol for triparental mating by Friday
- construct designs + primers by Sunday
Rushik recommended using pET15b (present in his lab)which has ampR, but marker must be replaced by specR or kanR - for cscABK in E coli