Team:IISER-Pune-India/TeamNotebook/Team Notebook 11e9906b405543a29361e38a50c81024/Meeting with Prem and Veeral - 5 abd4ec18130542bc83923de2f3f77c84

Meeting with Prem and Veeral - 5

Date	@June 27, 2021 11:00 AM
Department	Wet Lab
Description
HP sub-branch
Links/media
Participants
Property
Property 1
Property 2
Type	Meetings

• how much similarity is there between neutral site of 7942 and 2973, crispr is very precise so check that they're super similar

• salt in media will be an issue for quantifying stuff with HPLC, can use GCML for sucrose measurement instead, or the glucose-sucrose kit based assay.

• check sucrose production with vv strong (J23119), medium (cpc560) and weak 9J23119 Vo8 or 7), will also give an idea on the effect on membrane integrity.

• use any terminator doesn't matter (but I think the marburg2019 assay is good way to test the best terminator)

• gRNA+pam site (23 bp) shouldn't be there in the primers of the repair template (the cassette)

• use constitutive promoters to prevent additional cost or carbon use (or if you wanna use tunable ones, use light/carbon tunable ones)