Team:IISER-Pune-India/TeamNotebook/Team Notebook 11e9906b405543a29361e38a50c81024/Meeting with Gayathri 0cf3a30a18ed46649bcadc94c7880650
Meeting with Gayathri
Meeting with Gayathri
Date
Department
General
Description
Pitched the IP idea
HP sub-branch
Links/media
Participants
Property
Property 1
Property 2
Type
Meetings
Explaining stuff:
Random forestwhat are our analytes/requirements for them?how are we identifying/killing off the ones that got it wrong
we need to figure out the parts/biobricks we'll be using
make a proper presentation
the challenge for cloning a shorter gene into bacteria is different from that of a longer gene
give an idea of the timelines
"we can meet after a proper proposal"
she'll reply to mail with literature, reviews for genetic circuits
we can combine multiple of them maybe
about the 1ml/5ml concentrations, she think it's definitely possible, is tunable,
prev igem team lead detection system might have proposed it to be tunable
talk to abhinav masih
different colonies with negative feedbacks
namasi, arya ideaflagellar motilityadaptation circuit which resets it to the prev statee coli chemosensory pathwaywhen a ligand binds, the chemosensory mol gets methylated, which in turn switches on a pathway to phosphorylated, which tells it the flagellar motors to switch the directionquorum sensing also works by cross talk with dna bindingreferences on chemosensory systemsadaptation: it gets reset and hence detects a gradientit can only detect a relativephosphorylated protein as a transcription factor to switch on gene expression"two-component systems"
PhD students
previous year igem team she cant ask people to do it for us
She doesnt mind helping out for discussions
She doesn't want to be primary PI,
We need to talk to Thomas as we need a departmental decision to let us use lab facilities
50% rule, so we need to contact dept
To source strains, use references, for example, addgene for plasmids
biobricks,
you can contact people/authors of the paper, and people are usually open to sharing their strains for academic purposes
have backups ready in case you get hindered at the stage of finding a strain
