Team:IISER-Pune-India/TeamNotebook/Team Notebook 11e9906b405543a29361e38a50c81024/JC 3 7a07bdaef2f343848c57bebc06fa2100

JC 3

Date	@April 4, 2021 5:30 PM
Department	General
Description	Carbon Capture Papers and Wiki
HP sub-branch
Links/media
Participants	Jason JobyARYA NARNAPATTINAMASIVAYAM SANKARLIKHITH CHANDRAGIRIAAshwin UdaySoorya Narayan
Property
Property 1
Property 2
Type	JC

Material

Name	Type	Relevance	PPT	Assigned to	Due Date	Source
Carbon Capture pptx	Pptx	General Abstract of the Project				https://docs.google.com/presentation/d/1v67M4WST1HJHja9ZIa5vNBEI4G96tafX3gGnBLF757Q/edit#slide=id.gc649c6196e_6_10
Zhang (2020) - Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2	Paper	This is the main paper that our idea is based on. Using Cyanobacteria S. elongatus UTEX 2973 and E coli to produce 3-HP, an important metabolite that can be used to make bioplastics.	https://docs.google.com/presentation/d/1CGFu0ECMwsxZWyTugyyPJeSexsIsHbAwffouIavKbYQ/edit?ts=6068c50f#slide=id.p	LIKHITH CHANDRAGIRISoorya Narayan	@April 4, 2021	https://link.springer.com/article/10.1186/s13068-020-01720-0#Sec1
Lin (2020) - Enhanced production of sucrose in the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973	Paper	Latest paper on sucrose production in S. elongatus UTEX 2973, i think this may be the highest yield produced so far.	https://docs.google.com/presentation/d/16_-y_zUAwO5jbWWXuvee4lvCTZIkjzi3EmIvDSsC8oY/edit?usp=sharing	NAMASIVAYAM SANKARARYA NARNAPATTI	@April 4, 2021	https://www.nature.com/articles/s41598-019-57319-5#:~:text=In%20cyanobacterial%20cells%2C%20sucrose%20is,1A).&text=Engineering%20of%20Synechococcus%20elongatus%20UTEX%202973%20for%20production%20of%20sucrose.
Stony Brook - iGEM, 2018	Wiki	sucrose production in S. elongatus PCC 7942, light dependent inducer, iron based repressor, wanted to do directed evolution	https://docs.google.com/presentation/d/1y4fl-1TTdxEeFktUOVcSw0gr7c98R046NqI_JDCWZNg/edit?usp=sharing	Jason JobyAAshwin Uday	@April 5, 2021	https://2018.igem.org/Team:Stony_Brook

Arya, Namasi:

Can model the efficiency under vs without salt stress

Promoter characterisation can be done

What concentration of salt should the cyanobacteria be acclimatised to? why did they use 150 mM?

When is sucrose secreted? at what salt concentrations are other osmolytes sectreted?

Modelling carbon flux between sucrose and glycogen pathways

What are the optimal salt conc. levels, optimal levels of IPTG needed for induction and by extension, the optimal amounts of spp expression required

Is spp autoinduced? why is spp toxic?

Is Ptrc10 regulated by IPTG levels or no?

Why was the experiment done only for 5 days? We could model how frequently new cells need to be added to bioreactor if they need to be continually replenished.

Modelling the population dynamics of cyanobacteria and e coli. Should we use a kill switch in E coli to keep its population under control?

economic modelling

modelling optimal CO2 input needed for photobioreactor

Jason, Ashwin:

What was the problem with DNA synthesis? What sort of contamination took place?

SOOO many questions on Flux Based Analysis modelling

self shading and photoinhibition modelling

Should we write to the team?

Likhith, Soorya:

How was 3-HP secreted out?

availability of gas chromatography at IISER?

why was pH of 8.3 selected?

modelling optimal pH conditions and the functioning of the H+/sucrose symporter

sps is rds, so how to overexpress the native gene in synechococcus, read up on the Tx network for sps

Questions:

Whether we want synechococcus to secret sucrose under salt stress or in fresh water conditions

Do we induce sucrose secretion through light, Fe2+ levels etc.?

Do we acclimatise it to salt from the very beginning? Do we allow it to reach optimal cell density first before rerouting carbon flux away from glycogen to sucrose production?

What modelling should we do? What assays do we need to do?