Contributions
| Date | |
|---|---|
| Department | Wet Lab |
| Description | Possible contributions we can make for bronze criteria |
| HP sub-branch | |
| Links/media | |
| Participants | |
| Property | |
| Property 1 | |
| Property 2 | |
| Type | Deliverables |
Phytobrick stds: https://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks
make all parts moclo/phytobrick compatible as a contribution?
Cyanobacteria
CscB
http://parts.igem.org/Part:BBa_K2596005 - Stony Brook '18 - codon optimised cscB for 7942 (after optimisation there was a PSt1 site that they removed by a silent mutation)
Could add data to this part?
sucrose assays for this part: http://parts.igem.org/Part:BBa_K2596015
tRNA gene summary for utex 2973: http://gtrnadb.ucsc.edu/genomes/bacteria/Syne_UTEX_2973/Syne_UTEX_2973-summary-codon.html
Stanford biobrick: http://parts.igem.org/Part:BBa_K656011
characterised in PCC 7942 - Measurement of growth and sucrose export in cscB-transformed PCC7942 along with an assessment of transformation stability by omitment of antibiotic pressure
Possible contributions:
- Add literature (tho that'll be diffi to do since there's already a lot on the registry)
- Characterization: membrane integrity assay
- Information from literature
Pcpc 560
http://parts.igem.org/Part:BBa_K2596006 - - Stony Brook '18 - luciferase assay in PCC 6803
http://parts.igem.org/Part:BBa_K1968001 - Edinburg '16 - Promoter with phytobrick adaptors
http://parts.igem.org/Part:BBa_K2924000 - Dusseldorf '19 - did mVenus characterization BUT in PCC 6803.
http://parts.igem.org/Part:BBa_K2924036 - Dusseldorf -19 - composite mVenus part (mVenus codon optimised for pcc 6803)
Possible contributions:
- Add literature
- Characterize activity in different chassis (utex 2973)
- Can characterize activity under different light and carbon conditions
- Could codon optimize mVenus for utex (using 7942 table and compare, instead of an eyfp assay?)
- There is a native RBS, we could try and use different RBS with it and characterize strength with an eyfp assay - Attempts to modify the native RBS have not resulted in higher protein yields, so the native RBS was left in the part (Dusseldorf team comment on their part)
Double terminator
http://parts.igem.org/Part:BBa_B0015 - Antiquity '03
Possible contributions:
- Do fluorescence ratio assay in utex
PsbA1
http://parts.igem.org/Part:BBa_K754000 - Valencia '12
Possible contributions:
- Add literature
- Characterize activity in utex
- Measure activity in different light/carbon conditions
E.coli
CscB
http://parts.igem.org/Part:BBa_K656011 - stanford '11
Possible contributions:
- Add literature
- Isn't RFC 10 or 1000 complaint which are the registry de facto stds, so we can introduce silent mutations to make it compliant
- Characterize in E.coli (have to think of an assay)
CscA
http://parts.igem.org/Part:BBa_K917000 - edinburg '12 - characterized by viability assay on sucrose media
check if this sequence matches pcscX (98.68% identity), if yes can characterize (need to think of an assay)
Possible contributions:
- Add literature
- This is from Escherichia coli O157:H7 strain Sakai (E.coli W one will be a new part!)
CscK