Team:Edinburgh/Part Collection

The SuperGrinder






Part Collection

We developed a great collection of silica binding tags which can be assembled with any protein for immobilisation, purification or high-throughput screening. The tags are compatible with Phytobrick standard and designed for use in JUMP assembly (Golden Gate cloning). We also developed composite parts of tags assembled with sfGFP for characterisation of binding affinities/tag performance. This will allow other researchers to optimise their own protocols for immobilisation.

Name Type Description Designer Length
BBa_K3946003 Tag L2NC+linker (C) Fiona Bunn 432
BBa_K3946000 Tag SB7 silica tag (C) Ritupreet Kaur 28
BBa_K3946001 Tag Car9 silica tag (C) Fiona Bunn 55
BBa_K3946002 Tag L2NC silica tag (C) Fiona Bunn 377
BBa_K3946004 Tag L2 silica tag (C) Fiona Bunn 666
BBa_K3946008 Tag Sb7 silica tag (N) Ritupreet Kaur 28
BBa_K3946009 Tag Car9 silica tag (N) Fiona Bunn 64
BBa_K3946007 Conjugation L2NC+linker+term (CT) Fiona Bunn
BBa_K3946006 Conjugation L2NC silica tag+term (CT) Fiona Bunn 4461
BBa_K3946052 Coding L2NC+linker+sfGFP Fiona Bunn 1158
BBa_K3946050 Coding Car9 silica tag+sfGFP Fiona Bunn 783
BBa_K3946051 Coding L2NC silica tag+sfGFP Fiona Bunn 1105