Our project had the aim of creating a programmable cell factory for the production of fatty acids. That would allow us to control the fatty acid profiles in a tuneable fashion. Although we were unable to produce and regulate the fatty acid production in our wet lab work, we are confident that our work is substantial enough as a proof of concept.
The first critical point of our project was to integrate and test out the bacterial FAS system, that would allow us to regulate the expression of discrete, mono-functional thioesterases. Unfortunately, we were unable to integrate all of the eight required genes into the yeast strain, thus, not being able to show the results ourselves. However, in the study that inspired our project for integrating the bacterial FAS system in yeast (Fernandez‐Moya et al., 2015), they were able to show that the system works in Saccharomyces cerevisiae. Hence, it is with great confidence we can say that the same system would work in our project had we managed to integrate the genes as intended.
In addition, we successfully benchmarked all three chemical induction systems using fluorescent proteins carried on individual plasmids. Each system was verified individually, and in combination with the other two systems. As noted, we did not manage to fully integrate the fully functional bacterial FAS system into the yeast genome, so the thioesterase plasmids we designed were not tested.