The induction system are responsible for inducing expression of our desired proteins upon addition of the corresponding induction agent. The copper system is native to yeast and responds to CU2+ ions. The TetOn system is from bacterial antibiotic resistance and activates upon addition of tetracycline family antibiotics, requiring the presence of the rtTA-NLS-GAL4AD fusion protein, known as TetA.
Sequence constituively expressing estradiol-sensing protein required for estradiol induction
The yeast fatty acid synthase (FAS) system
The yeast fatty acid synthase (FAS) system cannot be targeted for protein engineering efficiently since it is expressed as two large genes that complex to provide function, so we replace it with a bacterial FAS2 system consiting of these 8 genes.
Estradiol signalling comes homo sapiens and is a hormone signalling system, responding to the hormone estradiol.
A Table Shows The yeast fatty acid synthase (FAS) system
As a part of our committment to future iGEM teams, we also characterised three ribosome binding sites (RBS) to provide additonal knowledge and options to future teams.
The characterisation was done on parts previously reported by other teams in the part registry.