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BBa_K108027

Xylene monooxygenase subunit 1 is a protein used to oxidize toluene and xylenes to(methyl)benzyl alcohols, and (methyl)benzyl alcohols to
(methyl)benzaldehydes, and indole to indoxyl.The whole subunit is composed of two subunits: XylA and XylM.

Usage and Biology

The induction rate of change of the Xylene monooxygenase in E.coli is shown in the graph below. There are 3 steps of biotransformation of xylene monooxygenase, adding toluene and pseudocumene can inhibit the second and third steps and there are certain alcohols that can inhibit the third step.

Sequence and Features

MDTLRYYLIPVVTACGLIGFYYGGYWVWLGAATFPALMVLDVILPKDFSARKVSPFFADLTQYLQLPLMIGLYGLLVFGVENG
RIELSEPLQVAGCILSLAWLSGVPTLPVSHELMHRRHWLPRKMAQLLAMFYGDPNRDIAHVNTHHLYLDTPLDSDTPYRGQTI
SFVISATVGSVKDAIKIEAETLRRKGQSPWNLSNKTYQYVALLLALPGLVSYLGGPALGLVTIASMIIAKGIVEGFNYFQHY
GLVRDLDQPILLHHAWNHMGTIVRPLGCEITNHINHHIDGYTRFYELRPEKEAPQMPSLFVCFLLGLIPPLWFALIAKPKLRD
WDQRYATPGERELAMAANKKAGWPLWCESELGRVASI

References

1.Bühler B, Schmid A, Hauer B, Witholt B. Xylene monooxygenase catalyzes the multistep oxygenation of toluene and pseudocumene to corresponding alcohols, aldehydes, and acids in Escherichia coli JM101. J Biol Chem. 2000 Apr 7;275(14):10085-92. doi: 10.1074/jbc.275.14.10085. PMID: 10744688.

2.https://www.uniprot.org/uniprot/P21395