Team:Austin UTexas/Contribution

Contributions



Our team decided to add documentation to the iGEM Registry regarding parts relevant to our project, including PETase, MHETase, and Rsn-2. While pages for these parts had basic information including function and origin, little information was present on potential applications of these parts. In particular, Rsn-2 had very little information on its page. To remedy this, we added information from current literature to each page in order to provide more context for each part’s use and significance. Information was formatted in paragraphs under a contribution header, along with references for sources of information.

PETase: BBa_K2010999 Provides info on thermostability of LCC PETase and included some charts from this on degradation rates over time, comment on how they level off due to breakdown of the enzyme itself. Additionally, we provided information on protein anchoring in yeast and compared the efficiencies of multiple types of PETase.

MHETase: BBa_K1921001 Originally, the Registry page for MHETase had no information on MHETase’s synergy with PETase. Thus, we provided information on how MHETase assists PETase in degrading PET plastic at higher rates. In particular, important information on appropriate combinations of MHETase and PETase for optimized degradation of plastic was provided.

Rsn-2: BBa_K660100 To improve Rsn-2’s part page, we included information on the protein’s ability to decrease surface tension at oil-water or air-water interfaces. A description of the mechanism facilitating this function was included, alongside information on Rsn-2’s ability as a surfactant to destroy biofilms.