Team:CUHKSZ
Background
Alcoholic beverages usually play an important role in our social life. Some spirits, like Chinese Maotai, may also contain some ingredients beneficial to human health. However, the high concentration of EthaNOl in those spirits can also take a serious toll on human health.
About EthaNO
Project Aim
We aim to figure out the oxidation mechanism of bi-functional alcohol/aldehyde dehydrogenase (AdhE) through computational biology E.coli bacteria are transformed with modified gene and will generate enzymes to degrade EthaNOl.
User Friendly
To make it user-friendly, PVA-SA granules are applied to store the bacteria in the device. We will optimize the immobilization method to maximize degradation efficiency.
Significance
With EthaNO, people can enjoy alcoholic beverages like Maotai without worrying about EthaNOl.
Wet Lab
Obtain Engineered E.coli. Obtain Culture System of Acetobacter Pasteurianus. Test the Metabolic Properties of Co-culture System
Dry Lab
After revising the technical misconducts, we docked in the order of “first NAD+ then ligand” to their domain respectively in their monomer.
Material
Almost zero immobilized bacteria in our water gel beads can leak out. Even if small number of bacteria is permeable, no food safety concern is needed.
Watch Video Learn More
Video description: EthaNO is a tiny device that can decompose EthaNOl in alcoholic beverages. The genetically modified bacteria within the device, which is harmless, can actively express the enzymes that degrade the alcohol into carbon dioxide rapidly. With EthaNO, people can enjoy the famous spirits like Maotai without worrying about EthaNOl.